10.57647/pibm.2023.122320

Construction and Characterization Of a 3D Scaffold Containing 30B Modified Clay Nanoparticles for Bone Tissue Engineering.

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  • Nikoo Yazdani Elahabadi1,
  • Shahrokh Shojaei*1,
  • Vahabodin Goodarzi2,
  1. Department of Biomedical Engineering, CT.C., Islamic Azad University, Tehran, Iran
  2. Applied Biotechnology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran

Received: 2023-08-07

Revised: 2023-11-05

Accepted: 2023-11-08

Published in Issue 2023-12-30

Copyright (c) 2025 Nikoo Yazdani Elahabadi, Shahrokh Shojaei, Vahabodin Goodarzi (Author)

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

How to Cite

Yazdani Elahabadi, N., Shojaei, S., & Goodarzi, V. (2023). Construction and Characterization Of a 3D Scaffold Containing 30B Modified Clay Nanoparticles for Bone Tissue Engineering. Progress in Biomaterials, 12(4). https://doi.org/10.57647/pibm.2023.122320
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Abstract

This study designs and evaluates 3D scaffolding using biodegradable polymers of polylactic acid, ‎polycaprolactone, hydroxyapatite, and 30B clay to address bone problems.

X-ray diffraction is employed to examine the crystal structure properties of the samples and the ‎phase of the diffraction plates. The morphological study of the surfaces of samples is conducted ‎with a field-emitting electron microscope (FESEM). The elemental analysis map test is utilized to ‎examine the elements in the compound structures. Cell viability and toxicity are evaluated using ‎the MTT method on the growth and reproduction of human-class MG-63 bone cells, as well as skin ‎fibroblast cells.

An analysis of the Fourier transform infrared spectroscopy shows that‏ ‏the interactions between ‎ingredients can be identified roughly based on their functional groups. Results demonstrate that the ‎scaffolding containing all four materials has an average porosity size of 8.40 µm and a standard ‎deviation of the diameter of the porosities of 6.31 µm. The volume percentage of porosities is ‎‎24.93%.

It can be concluded that the composition of all four materials forms a completely porous structure ‎that can benefit the final properties of the production scaffolding. The scaffold has sufficient mechanical properties to maintain the structure of damaged bone and facilitates the formation of small pores using the salt leaching process. DAPI coloring is used to check ‎the decellularization and core residue, showing favorable behavior.

Keywords

  • 3D scaffolding,
  • 30B clay nanoparticles,
  • Hydroxyapatite,
  • Polycaprolactone,
  • Polylactic acid
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