OICC Press Progress in Biomaterials 2194-0517 12 4 2023 12 30 Construction and Characterization Of a 3D Scaffold Containing 30B Modified Clay Nanoparticles for Bone Tissue Engineering. 10.57647/pibm.2023.122320 EN Nikoo Yazdani Elahabadi Department of Biomedical Engineering, CT.C., Islamic Azad University, Tehran, Iran Shahrokh Shojaei Department of Biomedical Engineering, CT.C., Islamic Azad University, Tehran, Iran Vahabodin Goodarzi Applied Biotechnology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran Journal Article 2023 12 30 This study designs and evaluates 3D scaffolding using biodegradable polymers of polylactic acid, ‎polycaprolactone, hydroxyapatite, and 30B clay to address bone problems. X-ray diffraction is employed to examine the crystal structure properties of the samples and the ‎phase of the diffraction plates. The morphological study of the surfaces of samples is conducted ‎with a field-emitting electron microscope (FESEM). The elemental analysis map test is utilized to ‎examine the elements in the compound structures. Cell viability and toxicity are evaluated using ‎the MTT method on the growth and reproduction of human-class MG-63 bone cells, as well as skin ‎fibroblast cells. An analysis of the Fourier transform infrared spectroscopy shows that‏ ‏the interactions between ‎ingredients can be identified roughly based on their functional groups. Results demonstrate that the ‎scaffolding containing all four materials has an average porosity size of 8.40 µm and a standard ‎deviation of the diameter of the porosities of 6.31 µm. The volume percentage of porosities is ‎‎24.93%. It can be concluded that the composition of all four materials forms a completely porous structure ‎that can benefit the final properties of the production scaffolding. The scaffold has sufficient mechanical properties to maintain the structure of damaged bone and facilitates the formation of small pores using the salt leaching process. DAPI coloring is used to check ‎the decellularization and core residue, showing favorable behavior.