Quantitative Analysis of β-Blockers in Water Samples by HPLC Using Directly Suspended Droplet Microextraction
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Abstract
The aim of this study is using Directly Suspended Droplet Microextraction (DSDME) as the sensitive drug assays in environmental waters. This work explains the success of this liquid-phase microextraction technique used as sample pre-concentration technique. This attractive method, which is selective and enable substantial pre-concentration, has been used for determination of three β-blockers; Atenolol, Metoprolol and Propranolol as the model compounds. The effective parameters such as organic solvent, pH of donor and acceptor phases, phase volumes, extraction and back-extraction time, stirring rate and addition of salt are discussed. The extracted β-blockers were analyzed at room temperature by High Performance Liquid Chromatography (HPLC), equipped an Agilent Eclipse XDB-C18 column with particle size of 5μm (250mm×4.6 mm i.d.) and programmed wavelength fluorescence detector. The mobile phase was 0.01molL-1 NaH2PO4 (adjusted to pH 3.0 with phosphoric acid)-methanol-acetonitril (45:45:10, v:v:v). The flow rate was 1.0 mLmin-1. The separated β-blockers were detected using fluorometric detection. The results showed that practical pre-concentration factors varied from 34.8 to 171.6.
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Volume 14 (2023) IJIC
Issue 2, October 2023
Page Numbers: 1-9
Article views: 38
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