Antibacterial effect of bioceramic root canal sealer (BioRootTM) modified with Thymus Kotschyanus Boiss

Authors

• Khalid Mohammed Sharif Zebari ¹
• Abdullkareem Ramazan AL-Mezory * ²
• Hadar Saaeed Faizy ²

1. Department of Conservative Dentistry, College of Dentistry, University of Duhok, Iraq
2. College of Agricultural Engineering Science, Duhok University, Iraq

Abstract

The present study aimed to evaluate the antibacterial efficacy of bioceramic root canal sealer
modified with herbal extracts, because there are insufficient scientific reports that indicate the
antibacterial effects of herbal extracts added to endodontic root canal sealers. The essential oils of
the aerial parts of Thymus Kotschyanus Boiss were obtained by hydrodistillation (HD) method
and analyzed by Gas Chromatography-Mass Spectrometry (GC/MS) Analysis, and the major
compounds were carvacrol (53.61) and thymol (12.78). The herbal extracts were added in different
percentages to BioRootTM root canal sealer to know the effective concentration against the E.
faecalis bacteria by placing into the agar base, and the zones of inhibitions were measured. The
groups were assessed as AH Plus (control), BioRootTM, and (BioRootTM+Thymus Kotschyanus
Boiss) Exp. Material (1-4)%, Exp. Material (1-2)%, Exp. Material (1-1)% and for evaluating the
antibacterial activity the agar diffusion test (ADT) was used. The AH Plus (control) exhibited the
largest inhibition zone 10.05 (0.99) mm, followed by Exp. Material (1-1)% 7.82(0.77) mm, Exp.
Material (1-2)% 4.96 (0.60) mm, while the least inhibition zone was found in BioRootTM 4.39
(0.55) mm and Exp. Material (1-4)% was 4.36 (0.59) mm. Using ANOVA one-way test, there was
a statistically significant difference of inhibition zone among root canal sealers. Based on results,
statistical analysis and observation of the AH Plus sealer and Exp. Material (1-1)% had higher
antibacterial effects than BioRootTM sealer, so the oil of Thymus Kotschyanus Boiss can be added
as one of the components of root canal sealer, but farther research is needed before recommending
it as sealers.

Keywords

• Agar diffusion test
• E. faecalis
• Antibacterial activity
• Thymus Kotschyanus Boiss
• BioRoot TM
• AH Plus