10.57647/ijrowa-fznx-zd35

Evaluating the Performance of Tea Leaf Waste as a Substrate Supplement for Cultivating Pleurotus ostreatus L. on Groundnut Shell

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  • Tedros Gebrezgiabhier Gebreyesus*1,
  • Kamal C. Semwal2,
  1. Department of Biology, Mai-Nefhi College of Science, Eritrea Institute of Technology, Asmara, Eritrea
  2. Department of Biology, Mai-Nefhi College of Science, Eritrea Institute of Technology, Asmara, Eritrea
Evaluating the Performance of Tea Leaf Waste as a Substrate Supplement for Cultivating Pleurotus ostreatus L. on Groundnut Shell

Received: 2024-08-26

Revised: 2024-09-25

Accepted: 2025-01-30

Published in Issue 2025-06-01

Copyright (c) -1 Tedros Gebrezgiabhier Gebreyesus, Kamal C. Semwal (Author)

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

How to Cite

Gebreyesus, T. G. ., & Semwal, K. C. . (2025). Evaluating the Performance of Tea Leaf Waste as a Substrate Supplement for Cultivating Pleurotus ostreatus L. on Groundnut Shell. International Journal of Recycling of Organic Waste in Agriculture, 14(3). https://doi.org/10.57647/ijrowa-fznx-zd35
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Abstract

Purpose: There is no recycling plan for a tea leftover waste which is available in very high quantities in Eritrea. In view of the growing importance of environmental sanitation, food security and mushroom recipe in Eritrea this study was conducted to evaluate tea leaves waste as substrate mixed with ground nut shell for Oyster mushroom (Pleurotus ostreatus L.) cultivation.

Method: Different substrate mixtures were prepared and filled in polythene bags and were autoclaved (121oc and 15Lbs) for 2hrs. Substrate bags were inoculated with Oyster spawn and incubated in a cultivation hat with controlled conditions. Growth parameters were recoded for each bag of each treatment and the data thus obtained were analysed statistically using R-software (R-4.3.3) at two significant levels (P < 0.05 and P < 0.01) and Chi-square test.

Results: In all replications 40% tea leaf waste supplied with 58% ground nut shell showed high performance in terms of mycelium colonization in bags, growth time, yield (189.9 ± 5.0g) and biological efficiency (47.5± 1.3%) close to the control group (200±0.39g, BE= 49± 0.1%). At p-value (p < 0.01), the assessed treatments showed a significant difference with P-value 4.74E-200. All of the growth parameters were statistically different, with p-value = 0.00 at p-value (p < 0.01).

Conclusion: Therefore, tea leaf waste with aforesaid percentage of groundnut shell can be used as alternative substrate, which will reduce the cost of cultivation of this prized mushroom and the technology can be transferred to the local farmers.

Research Highlights:

  • Exploiting agro-industrial wastes for mushroom cultivation is eco-friendly.
  • Using agro-wastes as substrates contributes on sanitation and food security.
  • Groundnut shell supplied with tea leaf waste exhibited satisfactory yield.
  • Spent tea waste (40%) can serve as alternative supplement to wheat bran.
  • This study promotes low cost cultivation for small scale mushroom farmers.

Keywords

  • Food security,
  • Growth parameters,
  • Performance,
  • Substrate,
  • Significant
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References

  1. Aditya N, Jarial R, Jarial K, Bhatia J (2024) Comprehensive review on oyster mushroom species (Agaricomycetes): Morphology, nutrition, cultivation and future aspects. Heliyon 10(5):e26539. https://doi.org/10.1016/j.heliyon.2024.e26539
  2. Ahmed R, Niloy M, Islam M, Reza M, Yesmin S, Rasul S, Khandakar J (2023) Optimizing tea waste as a sustainable substrate for oyster mushroom (Pleurotus ostreatus) cultivation: a comprehensive study on biological efficiency and nutritional aspect. Front Sustain Food Syst 7. https://doi.org/10.3389/fsufs.2023.130 8053
  3. Akcay C, Ceylan F, Arslan R (2023) Production of oyster mushroom (Pleurotus ostreatus) from some waste lignocellulosic materials and FTIR characterization of structural changes. Sci Rep 13(1). https://doi.org/ 10.1038/s41598-023-40200-x
  4. Alemu F (2013) Cultivation of Pleurotus ostreatus mushrooms on Coffea arabica and Ficus sycomorus leaves in Dilla University, Ethiopia. J Yeast Fungal Res 4(7): 103–108. https://doi.org/10.5897/JYFR2013.0123
  5. Ali S, Ali S, Laique A, Shafique M, Khan S, Atta S (2012) Mushroom growth on different media. Mycopath 10(1): 37–39.
  6. Anjana S, Savita J (2017) Oyster mushroom: Answer to human ailments. Asian J Pharm Clin Res (AJPCR) 10(4): 24–27. https://doi.org/10.22159/ajpcr.2017.v10i4.16867
  7. Atila F (2017) Evaluation of suitability of various agro-wastes for productivity of Pleurotus djamor, Pleurotus citrinopileatus and Pleurotus eryngii Mushrooms. J Exp Agric Int 17(5):1–11. https://doi.org/10.9734/jeai/ 2017/36346
  8. Barh A, Sharma V, Annepu S, Kamal S, Sharma S, Bhatt P (2019) Genetic improvement in Pleurotus (oyster mushroom): a review. Biotech 9(9). Springer Verlag. https://doi.org/10.1007/s13205-019-1854-x
  9. Baysal E, Peker H, Yalinkiliç M, Temiz A (2003) Cultivation of oyster mushroom on waste paper with some added supplementary materials. Bioresour Technol 89(1): 95–97. https://doi.org/10.1016/s0960-8524(03)00028-2
  10. Bellettini M, Fiorda F, Maieves H, Teixeira G, Ávila S, Hornung P, Júnior A, Ribani R (2019) Factors affecting mushroom Pleurotus spp. Saudi J Biol Sci 26 (4): 633–646. https://doi.org/10.1016/j.sjbs.2016.12.005
  11. Bhattacharjya D, Paul R, Miah M, Ahmed K (2014) Effect of different saw dust substrates on the growth and yield of oyster mushroom (Pleurotus ostreatus). IOSR-JAVS 7(2):38–46. https://doi.org/10.9790/2380-07233846
  12. Bilal S, Mushtaq A, Moinuddin K (2014) Effect of different grains and alternate substrates on oyster mushroom (Pleurotus ostreatus) production. Afr J Microbiol Res 8(14):1474–1479. https://doi.org/10.5897/ajmr2014. 6697
  13. Chen L, Qian L, Zhang X, Li J, Zhang Z, Chen X (2022) Research progress on indoor environment of mushroom factory. Int J Agric Biol Eng 15(1):25–32. https://doi.10.25165/j.ijabe.20221501.6872
  14. Chukowry D, Devi R, Lalljee B (2009) Evaluation of tea wastes as an alternative substrate for oyster mushroom cultivation. Univ Maurit Res J (15):458-473.
  15. Das N, Mukherjee M (2007) Cultivation of Pleurotus ostreatus on weed plants. Bioresour Technol 98(14):2723–2726. https://doi.org/10.1016/j.biortech.2006.09.061
  16. Dissasa G (2022) Cultivation of different oyster mushroom (Pleurotus spp.) on coffee waste and determination of their relative biological efficiency and pectinase enzyme production, Ethiopia. Int J Microbiol Res 2022: 5219939. https://doi.org/10.1155/2022/5219939
  17. Ejigu N, Sitotaw B, Girmay S, Assaye H (2022) Evaluation of oyster mushroom (Pleurotus ostreatus) production using Water hyacinth (Eichhornia crassipes) biomass supplemented with agricultural wastes. Int J Food Sci 2022. https://doi.org/10.1155/2022/9289043
  18. Elsakhawy T, Ramdan A, ElGabry K, Al-sharnouby S (2022) Production of oyster mushroom (Pleurotus ostreatus) and tracking the lignin degrading enzymes on different agro-industrial residues. Env Biodivers Soil Secur. 6(2022):319–326. https://doi:10.21608/jenvbs.2022.167666.1195
  19. Fernández-Fueyo E, Ruiz-Dueñas F, Jesús Martínez M, Romero A, Hammel K, Medrano F, Martínez A (2014) Ligninolytic peroxidase genes in the oyster mushroom genome: heterologous expression, molecular structure, catalytic and stability properties, and lignin-degrading ability. Biotechnol Biofuels 7(2). https://doi.org/10. 1186/1754-6834-7-2
  20. Fletcher I, Freer A, Ahmed A, Fitzgerald P (2019) Effect of temperature and growth media on mycelium growth of Pleurotus ostreatus and Ganoderma lucidum strains. J Microbiol Infect Dis (2). https://doi.org/10.31031/CJMI. 2019.02.000549
  21. Girmay Z, Gorems W, Birhanu G, Zewdie S (2016) Growth and yield performance of Pleurotus ostreatus (Jacq. Fr.) Kumm (oyster mushroom) on different substrates. AMB Express 6(1). https://doi.org/10.1186/s13568-016-0265-1
  22. Hasan G, Abdulhadi S (2022) Molecular characterization of wild Pleurotus ostreatus (MW457626) and evaluation of b-glucans polysaccharide activities. Karbala Int J Mod Sci 8(1):52–62. https://doi.org/10.33640/2405-609X.3204
  23. Hoa H, Wang C, Wang C (2015) The effects of different substrates on the growth, yield, and nutritional composition of two oyster mushrooms (Pleurotus ostreatus and Pleurotus cystidiosus). Mycobiology 43(4):423–434. https://doi.org/10.5941/MYCO.2015.43.4.423
  24. Kamthan R, Tiwari I (2017) Agricultural wastes- potential substrates for mushroom cultivation. European J Exp Biol 7(5). https://doi.org/10.21767/2248-9215.100031
  25. Kinge T (2016) Effect of local substrates on the growth and yield of Pleurotus ostreatus K. in the North West Region, Cameroon. Curr Res Environ Appl Mycol 6(1):11–19. https://doi.org/10.5943/cream/6/1/2
  26. Kumar P, Bharty S, Singh R, Kumar K, Rani N (2018) Impact of oyster mushroom (Pleurotus ostreatus) training on socio-economic and knowledge of tribal woman of Hazaribag, Jharkhand, India. Int J Curr Microbiol App Sci (7):1106-1111.
  27. Kurtzman R (2010) Pasteurization of mushroom substrate and other solids. Afr J Environ Sci Technol 4(13): 936–941.
  28. Mahadevan K, Shanmugasundaram K (2018) Comparative effect of different culture media on mycelial growth performance of Pleurotus sapidus. J Pharmacogn Phytochem 7(4): 874–878.
  29. Mamiro D, Royse D (2008) The influence of spawn type and strain on yield, size and mushroom solids content of Agaricus bisporus produced on non-composted and spent mushroom compost. Bioresour Technol 99(8):3205–3212. https://doi.org/10.1016/j.biortech.2007.05.073
  30. Markson A, Madunagu B, Akpan U, Eshiet E (2012) Growth influence of some additives on the mycelial growth and fruit body development of Pleurotus ostreatus (Jacq. Et.). JBAH 2(3).
  31. Mridu, Atri N (2017) Nutritional and nutraceutical characterization of three wild edible mushrooms from Haryana, India. Mycosphere 8(8):1035–1043. https://doi.org/10.5943/mycosphere/8/8/4
  32. Naraian R, Singh M, Ram S (2016) Supplementation of basal substrate to boost up substrate strength and oyster mushroom yield: An overview of substrates and supplements. Int J Curr Microbiol Appl Sci 5(5):543–553. https://doi.org/10.20546/ijcmas.2016.505.056
  33. Ogundele G, Salawu S, Abdulraheem I, Bamidele O (2017) Nutritional composition of oyster mushroom (Pleurotus ostreatus) grown on softwood (Daniella oliveri) sawdust and hardwood (Anogeissus leiocarpus) sawdust. Br J Appl Sci Technol 20(1):1–7. https://doi.org/10.9734/bjast/2017/28160
  34. Osunde M, Olayinka A, Fashina C, Torimiro N (2019) Effect of carbon-nitrogen ratios of lignocellulosic substrates on the yield of mushroom (Pleurotus pulmonarius). Open Access Lib J 06(10):1–8. https://doi.org/10.4236/oalib.1105777
  35. Pardo-Gimenez A, Pardo-González J, Cunha D (2017) Supplementation of high nitrogen Agaricus compost: Yield and mushroom quality. J Agric Sci Technol 19 (7): 1589 -1601.
  36. Raymond P, Mshandete A, Kivaisi A (2013) Cultivation of oyster mushroom (Pleurotus HK-37) on solid sisal waste fractions supplemented with cow dung manure. J Life Sci 4(1). https://doi.org/10.5296/jbls.v4i1.2975
  37. Sassine Y, Naim L, El Sebaaly Z, Abou Fayssal S, Alsanad M, Yordanova M (2021) Nano urea effects on Pleurotus ostreatus nutritional value depending on the dose and timing of application. Sci Rep 11(1). https://doi.org/10 .1038/s41598-021-85191-9
  38. Sharma V, Singh M, Kumar R, Kumar S, Kamal S, Sharma M (2014) Effect of spawn to spawn multiplication on productivity of Agaricus bisporus. Mushroom Res 23(1):27-30.
  39. Thong-un N, Wongsaroj W (2022) Productivity enhancement using low-cost smart wireless programmable logic controllers: A case study of an oyster mushroom farm. Comput Electron Agric 195:106798. https://doi.org/10.1016/J.COMPAG.2022.106798
  40. Tupamahu C, Budiarso T (2017) The effect of oyster mushroom (Pleurotus ostreatus) powder as prebiotic agent on yoghurt quality. AIP Conf Proc 1844 (030006). https://doi.org/10.1063/1.4983433
  41. Upadhyaya J, Raut, J, Koirala N (2017) Analysis of Nutritional and Nutraceutical Properties of Wild-Grown Mushrooms of Nepal. EC Microbiology 12(3):136-145.
  42. Valenzuela-Cobos J, Guevara-Viejó F, Grijalva-Endara A, Vicente-Galindo P, Galindo-Villardón P (2023) Production and evaluation of Pleurotus spp. hybrids cultivated on Ecuadorian agro-industrial wastes: Using multivariate statistical methods. Sustainability 15(21): 15546. https://doi.org/10.3390/su152115546
  43. Victor Familoni T, Olusola Ogidi C, Juliet Akinyele B, Kayode O (2018) Evaluation of yield, biological efficiency and proximate composition of Pleurotus spp. cultivated on different wood dusts. CZECH Mycology, 70(1): 33–45.
  44. Wang L, Gao W, Wu X, Zhao M, Qu J, Huang C, Zhang J (2018) Genome-wide characterization and expression analyses of Pleurotus ostreatus MYB transcription factors during developmental stages and under heat stress based on de novo sequenced genome. Int J Mol Sci 19(7) 2052. https://doi.org/10.3390/ijms19072052
  45. Yamauchi M, Sakamoto M, Yamada M, Hara H, Mat T, Rezania S, Mohd F, Mohd H (2019) Cultivation of oyster mushroom (Pleurotus ostrreatus) on fermented moso bamboo sawdust. J King Saud Uni Sci 31(4):490–494. https://doi.org/10.1016/j. jksus. 2018.04.021
  46. Yang D, Liang J, Wang Y, Sun F, Tao H, Xu Q, Zhang L, Zhang Z, Ho C, Wan X (2016) Tea waste: an effective and economic substrate for oyster mushroom cultivation. J Sci Food Agric 96(2):680–684. https://doi.org/10. 1002/jsfa.7140
  47. Yang Y, Pia Y, Li J, Xu L, Lu Z, Dai Y, Li Q (2023) Integrative analysis of genome and transcriptome reveal the genetic basis of high temperature tolerance in Pleurotus giganteus (Berk. Karun & Hyde). BMC Genomics 24(552). https://doi.org/10.1186/s12864-023-09669-8
  48. Yoneyama S, Maeda K, Sadamori A, Saitoh S, Tsuda M, Azuma T, Nagano A, Tomiyama T, Matsumoto T (2021) Construction of a genetic linkage map and detection of quantitative trait locus for the ergothioneine content in tamogitake mushroom (Pleurotus cornucopiae var. citrinopileatus). Mycoscience 62(1):71–80. https://doi.org/ 10.47371/mycosci.2020.11.003
  49. Zhang J, Zhuo X, Wang Q, Ji H, Chen H, Hao H (2023) Effects of different nitrogen levels on lignocellulolytic enzyme production and gene expression under straw-state cultivation in Stropharia rugosoannulata. Int J Mol Sci 24(12):10089. https://doi.org/10.3390/ijms241210089
  50. Zhang W, Liu S, Kuang Y, Zheng, S (2019) Development of a novel spawn (block spawn) of an edible mushroom, Pleurotus ostreatus, in liquid culture and its cultivation evaluation. Mycobiology 47(1):97–104. https://doi.org/ 10.1080/12298093.2018.1552648
  51. Zou Y, Zhang M, Qu J, Zhang J (2018) ITRAQ-based quantitative proteomic analysis reveals proteomic changes in mycelium of Pleurotus ostreatus in response to heat stress and subsequent recovery. Front Microbiol 9:2368. https://doi.org/10.3389/fmicb.2018.02368