10.1186/2194-0517-2-5

Enzyme-modified indium tin oxide microelectrode array-based electrochemical uric acid biosensor

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  • Nidhi Puri1,
  • Vikash Sharma1,
  • Vinod K Tanwar1,
  • Nahar Singh1,
  • Ashok M Biradar1,
  • Rajesh*1,
  1. Polymer and Soft Material Section, CSIR-National Physical Laboratory, New Delhi, 110012, IN
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Published in Issue 2013-02-22

How to Cite

Puri, N., Sharma, V., Tanwar, V. K., Singh, N., Biradar, A. M., & Rajesh, . (2013). Enzyme-modified indium tin oxide microelectrode array-based electrochemical uric acid biosensor. Progress in Biomaterials, 2(1 (December 2013). https://doi.org/10.1186/2194-0517-2-5
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Abstract

Abstract We fabricated a miniaturized electrochemical uric acid biosensor with a 3-aminopropyltriethoxysilane (APTES)-modified indium tin oxide (ITO) microelectrode array (μEA). The ITO-μEA on a glass plate was immobilized with the enzyme uricase, through a cross-linker, bis[sulfosuccinimidyl]suberate (BS 3 ). The enzyme-immobilized electrode (uricase/BS 3 /APTES/ITO-μEA/glass) was characterized by atomic force microscopy and electrochemical techniques. The cyclic voltammetry and impedance studies show an effective binding of uricase at the μEA surface. The amperometric response of the modified electrode was measured towards uric acid concentration in aqueous solution (pH 7.4), under microfluidic channel made of polydimethylsiloxane. The μEA biosensor shows a linear response over a concentration range of 0.058 to 0.71 mM with a sensitivity of 46.26 μA mM −1 cm −2 . A response time of 40 s reaching a 95% steady-state current value was obtained. The biosensor retains about 85% of enzyme activity for about 6 weeks. The biosensor using μEA instead of a large single band of electrode allows the entire core of the channel to be probed though keeping an improved sensitivity with a small volume of sample and reagents.

Keywords

  • Uric acid,
  • Self-assembled monolayer,
  • Microfluidic,
  • PDMS,
  • Amperometric sensor
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