10.1186/2194-0517-2-4

Structure and biocompatibility of poly(vinyl alcohol)-based and agarose-based monolithic composites with embedded divinylbenzene-styrene polymeric particles

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  • Lydia G Berezhna*1,
  • Alexander E Ivanov1,
  • André Leistner2,
  • Anke Lehmann2,
  • Maria Viloria-Cols1,
  • Hans Jungvid1,
  1. Protista Biotechnology AB, Bjuv, SE-26722, SE
  2. Polymerics GmbH, Berlin, D-12681, DE
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Published in Issue 2013-02-21

How to Cite

Berezhna, L. G., Ivanov, A. E., Leistner, A., Lehmann, A., Viloria-Cols, M., & Jungvid, H. (2013). Structure and biocompatibility of poly(vinyl alcohol)-based and agarose-based monolithic composites with embedded divinylbenzene-styrene polymeric particles. Progress in Biomaterials, 2(1 (December 2013). https://doi.org/10.1186/2194-0517-2-4
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Abstract

Abstract Macroporous monolithic composites with embedded divinylbenzene-styrene (DVB-ST) polymeric particles were prepared by cryogelation techniques using poly(vinyl alcohol) or agarose solutions. Scanning electron microscopy images showed multiple interconnected pores with an average diameter in the range of 4 to 180 μm and quite homogeneous distribution of DVB-ST particles in the composites. Biocompatibility of the composites was assessed by estimation of the C5a fragment of complement in the blood serum and concentration of fibrinogen in the blood plasma which contacted the composites. A time-dependent generation of C5a fragment indicated weak activation of the complement system. At the same time, the difference in fibrinogen concentration, one of the most important proteins in the coagulation system of the blood, between the pristine blood plasma and the plasma, circulated through the monolithic columns, was insignificant.

Keywords

  • Cryogels,
  • Poly(vinyl alcohol),
  • Agarose,
  • Divinylbenzene-styrene particles,
  • C5a fragment of complement,
  • Fibrinogen
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References

  1. Arima et al. (2009) Complement activation by polymers carrying hydroxyl groups (pp. 2400-2407) https://doi.org/10.1021/am9005463
  2. Beugeling (1979) The interaction of polymer surfaces with blood (pp. 419-428)
  3. Black and Sefton (2000) Complement activation by PVA as measured by ELIFA (enzyme-linked immunoflow assay) for SC5b-9 (pp. 2287-2294) https://doi.org/10.1016/S0142-9612(00)00155-1
  4. Bolgen et al. (2007) Cryogelation for preparation of novel biodegradable tissue-engineering scaffolds (pp. 1165-1179) https://doi.org/10.1163/156856207781554064
  5. Brash et al. (1988) Mechanism of transient adsorption from plasma to solid surfaces: role of the contact and fibrinolytic systems (pp. 932-939)
  6. Craddock et al. (1977) Pulmonary vascular leukostasis resulting from complement activation by dialyzer cellophane membranes (pp. 879-888) https://doi.org/10.1172/JCI108710
  7. Dainiak et al. (2004) Integrated isolation of antibody fragments from microbial cell culture fluids using supermacroporous cryogels 1045(A) (pp. 93-98) https://doi.org/10.1016/j.chroma.2004.06.029
  8. Dainiak et al. (2010) Gelatin–fibrinogen cryogel dermal matrices for wound repair: preparation, optimisation and in vitro study (pp. 67-76) https://doi.org/10.1016/j.biomaterials.2009.09.029
  9. Unknown (2009) National Standards Authority of Ireland
  10. Fehra and Jacob (1977) In vitro granulocyte adherence and in vivo margination: two associated complement-dependent functions (pp. 641-652) https://doi.org/10.1084/jem.146.3.641
  11. Hanora et al. (2005) Screening of peptide affinity tags using immobilised metal affinity chromatography in 96-well plate format 1087(A) (pp. 38-44) https://doi.org/10.1016/j.chroma.2005.04.029
  12. Ivanov et al. (2012) Activated carbons and carbon-containing poly(vinyl alcohol) cryogels: characterization, protein adsorption and possibility of myoglobin clearance (pp. 16267-16278) https://doi.org/10.1039/c2cp42869e
  13. Jones and Czaja (1998) Intracellular signaling in response to toxic liver injury 275(G) (pp. 874-878)
  14. Kirkpatrick et al. (1998) Current trends in biocompatibility testing (pp. 75-84) https://doi.org/10.1243/0954411981533845
  15. Kirsebom et al. (2009) Mechanism of cryopolymerization: diffusion-controlled polymerization in a nonfrozen microphase (pp. 5208-5214) https://doi.org/10.1021/ma900566d
  16. Koc et al. (2011) Selective removal of 17β-estradiol with molecularly imprinted particle-embedded cryogel systems (pp. 1819-1826) https://doi.org/10.1016/j.jhazmat.2011.07.017
  17. Le Noir et al. (2007) Macroporous molecularly imprinted polymer/cryogel composite systems for the removal of endocrine disrupting trace contaminants 1154(A) (pp. 158-164) https://doi.org/10.1016/j.chroma.2007.03.064
  18. Lyle et al. (2010) Screening biomaterials for functional complement activation in serum 92(A) (pp. 205-213) https://doi.org/10.1002/jbm.a.32281
  19. Özgür et al. (2011) PHEMA cryogel for in-vitro removal of anti-dsDNA antibodies from SLE plasma (pp. 915-920) https://doi.org/10.1016/j.msec.2011.02.012
  20. Paradossi et al. (2003) Poly(vinyl alcohol) as versatile biomaterial for potential biomedical application (pp. 687-691) https://doi.org/10.1023/A:1024907615244
  21. Plieva et al. (2006) Pore structure of macroporous monolithic cryogels prepared from poly(vinyl alcohol) (pp. 1057-1066) https://doi.org/10.1002/app.23200
  22. Plieva et al. (2007) Macroporous gels prepared at subzero temperatures as novel materials for chromatography of particulate-containing fluids and cell culture applications (pp. 1657-1671) https://doi.org/10.1002/jssc.200700127
  23. Pulanic and Rudan (2005) The past decade: fibrinogen (pp. 341-349)
  24. Saich et al. (2007) Characterization of pro-apoptotic effect of liver failure plasma on primary human hepatocytes and its modulation by molecular adsorbent recirculation system therapy (pp. 732-742) https://doi.org/10.1111/j.1525-1594.2007.00447.x
  25. Sarma and Ward (2010) The complement system (pp. 227-235) https://doi.org/10.1007/s00441-010-1034-0
  26. Unknown (2007) Brochure of Toray Industries
  27. Tsai et al. (1999) Human plasma fibrinogen adsorption and platelet adhesion to polystyrene (pp. 130-139) https://doi.org/10.1002/(SICI)1097-4636(199902)44:2<130::AID-JBM2>3.0.CO;2-9
  28. Varoni et al. (2012) Agarose gel as biomaterial or scaffold for implantation surgery: characterization, histological and histomorphometric study on soft tissue response 53(6) (pp. 548-554) https://doi.org/10.3109/03008207.2012.712583
  29. Vogler and Siedlecki (2009) Contact activation of blood plasma coagulation (pp. 1857-1869) https://doi.org/10.1016/j.biomaterials.2008.12.041
  30. Vroman et al. (1980) Interaction of high molecular weight kininogen, factor XII and fibrinogen in plasma at interfaces (pp. 156-159)
  31. Wasilewska et al. (2009) Structure of fibrinogen in electrolyte solutions derived from dynamic light scattering (DLS) and viscosity measurements (pp. 3698-3704) https://doi.org/10.1021/la803662a
  32. Weber et al. (2008) Neutral styrene divinylbenzene copolymers for adsorption of toxins in liver failure (pp. 1322-1328) https://doi.org/10.1021/bm701396n
  33. Yuanyuan et al. (2001) Complement activation in factor D-deficient mice 98(25) (pp. 14577-14582) https://doi.org/10.1073/pnas.261428398