10.1186/2194-0517-2-2

Evaluation of polyphenylene ether ether sulfone/nanohydroxyapatite nanofiber composite as a biomaterial for hard tissue replacement

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  • Manickam Ashokkumar1,
  • Dharmalingam Sangeetha*1,
  1. Department of Chemistry, Anna University, Chennai, Tamil Nadu, 600025, IN
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Published in Issue 2013-02-06

How to Cite

Ashokkumar, M., & Sangeetha, D. (2013). Evaluation of polyphenylene ether ether sulfone/nanohydroxyapatite nanofiber composite as a biomaterial for hard tissue replacement. Progress in Biomaterials, 2(1 (December 2013). https://doi.org/10.1186/2194-0517-2-2
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Abstract

Abstract The present work is aimed at investigating the mechanical and in vitro biological properties of polyphenylene ether ether sulfone (PPEES)/nanohydroxyapatite (nHA) composite fibers. Electrospinning was used to prepare nanofiber composite mats of PPEES/nHA with different weight percentages of the inorganic filler, nHA. The fabricated composites were characterized using Fourier transform infrared spectroscopy (FTIR)-attenuated total reflectance spectroscopy (ATR) and scanning electron microscopy (SEM)-energy dispersive X-ray spectroscopy (EDX) techniques. The mechanical properties of the composite were studied with a tensile tester. The FTIR-ATR spectrum depicted the functional group as well as the interaction between the PPEES and nHA composite materials; in addition, the elemental groups were identified with EDX analysis. The morphology of the nanofiber composite was studied by SEM. Tensile strength analysis of the PPEES/nHA composite revealed the elastic nature of the nanofiber composite reinforced with nHA and suggested significant mechanical strength of the composite. The biomineralization studies performed using simulated body fluid with increased incubation time showed enhanced mineralization, which showed that the composites possessed high bioactivity property. Cell viability of the nanofiber composite, studied with osteoblast (MG-63) cells, was observed to be higher in the composites containing higher concentrations of nHA.

Keywords

  • Polyphenylene ether ether sulfone,
  • Nanohydroxyapatite,
  • Bioactivity,
  • Biomineralization,
  • Osteoblast cells
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References

  1. Bhattarai et al. (2005) Electrospun chitosan-based nanofibers and their cellular compatibility (pp. 6176-6184) https://doi.org/10.1016/j.biomaterials.2005.03.027
  2. Bismarck et al. (2001) Surface characterization of natural fibers: surface properties and water up-take behavior of modified sisal and coir fibers (pp. 100-107) https://doi.org/10.1039/b100365h
  3. Broza et al. (2005) Processing and assessment of poly(butylene terephthalate) nanocomposites reinforced with oxidized single wall carbon nanotubes (pp. 5860-5867) https://doi.org/10.1016/j.polymer.2005.05.073
  4. Bunsell et al. (2005) Fiber reinforced composite materials CRC Press https://doi.org/10.1201/9781420056969
  5. Darrell et al. (2006) Polymeric nanofibers: introduction ACS Symposium Series
  6. Dong et al. (2010) Bone regeneration with BMP-2 gene-modified mesenchymal stem cells seeded on nano hydroxyapatite/collagen/poly(l-lactic acid) scaffolds (pp. 547-566) https://doi.org/10.1177/0883911510380436
  7. Dahe et al. (2011) The biocompatibility and separation performance of antioxidative polysulfone/vitamin E TPGS composite hollow fiber membranes (pp. 352-365) https://doi.org/10.1016/j.biomaterials.2010.09.005
  8. Greiner and Wendorff (2007) Electrospinning: a fascinating method for the preparation of ultrathin fibers (pp. 5670-5703) https://doi.org/10.1002/anie.200604646
  9. Huang et al. (2003) A review on polymer nanofibers by electrospinning and their applications in nanocomposites (pp. 2223-2253) https://doi.org/10.1016/S0266-3538(03)00178-7
  10. Kalambettu et al. (2012) The effect of chlorotrimethylsilane on bonding of nano hydroxyapatite with a chitosan–polyacrylamide matrix (pp. 143-150) https://doi.org/10.1016/j.carres.2011.12.027
  11. Kang et al. (2008) Apatite-coated poly(lactic-co-glycolic acid) microspheres as an injectable scaffold for bone tissue engineering (pp. 747-756) https://doi.org/10.1002/jbm.a.31572
  12. Kim et al. (2004) The mechanism of biomineralization of bone-like apatite on synthetic hydroxyapatite: an in vitro assessment (pp. 17-22) https://doi.org/10.1098/rsif.2004.0003
  13. Lee et al. (2007) The effect of surface-modified nano-hydroxyapatite on biocompatibility of poly(ε-caprolactone)/hydroxyapatite nanocomposites (pp. 1602-1608) https://doi.org/10.1016/j.eurpolymj.2007.02.030
  14. Leonor et al. (2007) Functionalization of different polymers with sulfonic groups as a way to coat them with biomimetic apatite layer (pp. 1923-1930) https://doi.org/10.1007/s10856-007-3106-6
  15. Li and Xia (2004) Electrospinning of nanofibers: reinventing the wheel? (pp. 1151-1170) https://doi.org/10.1002/adma.200400719
  16. Liu and Tirrell (2008) Cell response to RGD density in crosslinked artificial extracellular matrix protein films 9(11) (pp. 2984-2988) https://doi.org/10.1021/bm800469j
  17. Lu et al. (2003) Three-dimensional, bioactive, biodegradable, polymer–bioactive glass composite scaffolds with improved mechanical properties support collagen synthesis and mineralization of human osteoblast-like cells in vitro (pp. 465-474) https://doi.org/10.1002/jbm.a.10399
  18. Mossman (1983) Rapid colorimetric assay for cellular growth and survival, application to proliferation and cytotoxicity assays (pp. 55-63) https://doi.org/10.1016/0022-1759(83)90303-4
  19. Peter et al. (2010) Preparation and characterization of chitosan–gelatin/nanohydroxyapatite composite scaffolds for tissue engineering applications (pp. 687-694) https://doi.org/10.1016/j.carbpol.2009.11.050
  20. Pielichowska and Blazewicz (2010) Bioactive polymer/hydroxyapatite (nano)composites for bone tissue regeneration (pp. 97-207) https://doi.org/10.1007/12_2010_50
  21. Ramakrishna et al. (2006) Electrospun nanofibers: solving global issues (pp. 40-50) https://doi.org/10.1016/S1369-7021(06)71389-X
  22. Reneker and Chun (1996) Nanometer diameter fibers of polymer, produced by electrospinning (pp. 216-223) https://doi.org/10.1088/0957-4484/7/3/009
  23. Ryszkowska et al. (2010) Biodegradable polyurethane composite scaffolds containing Bioglass for bone tissue engineering (pp. 1894-1908) https://doi.org/10.1016/j.compscitech.2010.05.011
  24. Salerno et al. (2010) Novel 3D porous multi-phase composite scaffolds based on PCL, thermoplastic zein and ha prepared via supercritical CO2 foaming for bone regeneration (pp. 1838-1846) https://doi.org/10.1016/j.compscitech.2010.06.014
  25. Seal et al. (2001) Polymeric biomaterials for tissue and organ regeneration (pp. 147-230) https://doi.org/10.1016/S0927-796X(01)00035-3
  26. Sgouras and Duncan (1990) Methods for the evaluation of biocompatibility of soluble synthetic polymers which have potential for biomedical use: 1—use of the tetrazolium-based colorimetric assay (MTT) as a preliminary screen for evaluation of in vitro cytotoxicity 1(2) (pp. 61-68) https://doi.org/10.1007/BF00839070
  27. Shanmuga Sundar and Sangeetha (2012) Investigation on sulphonated PEEK beads for drug delivery, bioactivity and tissue engineering applications (pp. 2736-2742) https://doi.org/10.1007/s10853-011-6100-9
  28. Sila-Asna et al. (2007) Osteoblast differentiation and bone formation gene expression in strontium-inducing bone marrow mesenchymal stem cell 53(1) (pp. 25-35)
  29. Sista et al. (2011) The influence of surface energy of titanium-zirconium alloy on osteoblast cell functions in vitro
  30. Srinivasan et al. (2012) Biocompatible alginate/nano bioactive glass ceramic composite scaffolds for periodontal tissue regeneration 87(1) (pp. 274-283) https://doi.org/10.1016/j.carbpol.2011.07.058
  31. Tan et al. (2003) Scaffold development using selective laser sintering of polyetheretherketone-hydroxyapatite biocomposite blends (pp. 3115-3123) https://doi.org/10.1016/S0142-9612(03)00131-5
  32. Teoh and Teoh (2004) Introduction to biomaterials engineering and processing—an overview World Scientific https://doi.org/10.1142/9789812562227
  33. Thangakumaran et al. (2009) Osteoblast response (initial adhesion and alkaline phosphatase activity) following exposure to a barrier membrane/enamel matrix derivative combination 20(1) (pp. 7-12) https://doi.org/10.4103/0970-9290.49048
  34. Wang (2003) Developing bioactive composite materials for tissue replacement (pp. 2133-2151) https://doi.org/10.1016/S0142-9612(03)00037-1
  35. Zebarjad et al. (2011) A study on mechanical properties of PMMA/hydroxyapatite nanocomposite (pp. 795-801) https://doi.org/10.4236/eng.2011.38096