10.1007/s40204-017-0069-7

Investigation of the effects of starch on the physical and biological properties of polyacrylamide (PAAm)/starch nanofibers

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  • Hadi Taghavian1,
  • Seyed-Omid Ranaei-Siadat2,
  • Mohammad Reza Kalaee*1,
  • Saeedeh Mazinani3,
  1. Department of Polymer Engineering, Islamic Azad University, Tehran, 1777613651, IR
  2. Nanobiotechnology Engineering Laboratory, Department of Biotechnology, Faculty of Energy Engineering and New Technologies, Shahid Beheshti University, GC, Tehran, IR
  3. Amirkabir Nanotechnology Research Institute (ANTRI), Amirkabir University of Technology, Tehran, IR
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Published in Issue 2017-07-26

How to Cite

Taghavian, H., Ranaei-Siadat, S.-O., Kalaee, M. R., & Mazinani, S. (2017). Investigation of the effects of starch on the physical and biological properties of polyacrylamide (PAAm)/starch nanofibers. Progress in Biomaterials, 6(3 (September 2017). https://doi.org/10.1007/s40204-017-0069-7
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Abstract

Abstract Here, we report the development of a new polyacrylamide (PAAm)/starch nanofibers’ blend system and highlight its potential as substrate for efficient enzyme immobilization. PAAm was synthesized and blended with starch. The final blend was then electrospun into nanofibers. The response surface methodology was used to analyze the parameters that control nanofiber’s diameter. Electrospun mat was then modified either by cross-linking or phytase immobilization using silane coupling agent and glutaraldehyde chemistry. Physico-chemical properties of blends were investigated using spectroscopic and thermal studies. The evaluation of immobilized enzyme kinetics on both pure and the starch blended PAAm nanofibers was performed using Michaelis–Menten kinetic curves. Fourier transform infrared spectroscopy results along with differential scanning and X-ray diffraction confirmed that blending was successfully accomplished. TGA analysis also demonstrated that the presence of starch enhances the thermal degradability of PAAm nanofibers. Finally, it was shown that addition of starch to PAAm increases the efficacies of enzyme loading and, therefore, significantly enhances the activity as well as kinetics of the immobilized enzyme on electrospun blend mats.

Keywords

  • Starch,
  • Nanofibers,
  • Polyacrylamide,
  • Enzyme immobilization,
  • Biological property
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