10.1007/s40204-014-0032-9

Magnesium corrosion particles do not interfere with the immune function of primary human and murine macrophages

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  • Isabelle Roth1,
  • Stephan Schumacher*2,
  • Tina Basler3,
  • Kathrin Baumert4,
  • Jan-Marten Seitz5,
  • Florian Evertz6,
  • Peter Paul Müller7,
  • Wolfgang Bäumer8,
  • Manfred Kietzmann2,
  1. Institute of Pharmacology, Toxicology and Pharmacy, University of Veterinary Medicine Hannover, Hannover, 30559, DE Elanco Animal Health, Lilly Deutschland GmbH, Bad Homburg, 61352, DE
  2. Institute of Pharmacology, Toxicology and Pharmacy, University of Veterinary Medicine Hannover, Hannover, 30559, DE
  3. Institute of Microbiology, University of Veterinary Medicine Hannover, Hannover, 30559, DE
  4. Division of Immunodermatology and Allergy Research, Clinic for Dermatology, Allergy and Venereology, Hannover Medical School, Hannover, 30625, DE
  5. Institute of Materials Science, Leibniz Universität Hannover, Hannover, 30823, DE
  6. Institute of Multiphase Processes, Leibniz Universität Hannover, Hannover, 30167, DE
  7. Helmholtz Centre for Infection Research, Brunswick, 38124, DE
  8. MBS Department, NCSU College of Veterinary Medicine, Raleigh, NC, 27607, US
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Published in Issue 2014-12-06

How to Cite

Roth, I., Schumacher, S., Basler, T., Baumert, K., Seitz, J.-M., Evertz, F., Müller, P. P., Bäumer, W., & Kietzmann, M. (2014). Magnesium corrosion particles do not interfere with the immune function of primary human and murine macrophages. Progress in Biomaterials, 4(1 (March 2015). https://doi.org/10.1007/s40204-014-0032-9
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Abstract

Abstract Magnesium is currently under investigation as a prospective biodegradable implant material. Biodegradation of magnesium causes a release of magnesium, hydroxide ions and hydrogen gas but it can also lead to the formation of particulate debris. Implant-derived particles may have immunotoxic effects. To investigate the influence of magnesium-derived particles on the immune functions of primary macrophages, up to 500 μg/ml magnesium or magnesium corrosion particles were added to the cell culture medium. No major effects were observed on cell viability and on the release of the proinflammatory cytokine tumor necrosis factor (TNF)α. In addition, the ability of macrophages to stimulate proliferation of allogenic lymphocytes in a mixed leukocyte reaction remained unaffected. When macrophages were incubated with magnesium particles and then infected with the apathogenic Mycobacterium smegmatis , infection-induced TNFα secretion from murine macrophages was inhibited but not from human macrophages. However, the bactericidal activity of either cell type was not influenced. In conclusion, magnesium-related particles did not restrict the immune function of macrophages, suggesting that magnesium implants and corrosion particles derived thereof are highly biocompatible and have a low inflammatory potential.

Keywords

  • Degradation,
  • Immunotoxic,
  • Infection,
  • Inflammation,
  • In vitro,
  • Phagocytes
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