10.1007/s40204-014-0028-5

Antibacterial effects of silver–zirconia composite coatings using pulsed laser deposition onto 316L SS for bio implants

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  • G. Pradhaban1,
  • Gobi Saravanan Kaliaraj*1,
  • Vinita Vishwakarma1,
  1. Centre for Nanoscience and Nanotechnology, Sathyabama University, Chennai, 600119, IN
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Published in Issue 2014-11-14

How to Cite

Pradhaban, G., Kaliaraj, G. S., & Vishwakarma, V. (2014). Antibacterial effects of silver–zirconia composite coatings using pulsed laser deposition onto 316L SS for bio implants. Progress in Biomaterials, 3(2-4 (December 2014). https://doi.org/10.1007/s40204-014-0028-5
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Abstract

Abstract Bacterial invasion on biomedical implants is a challenging task for long-term and permanent implant fixations. Prevention of initial bacterial adherence on metallic implants is an important concern to avoid extracellular matrix (biofilm) secretion from bacteria that is resistant to antibacterial agents. In order to overcome this defect, recently, surface coatings such as zirconia (ZrO 2 ) with higher smoothness have been shown to improve implants durability. In the present study, pulsed laser deposition (PLD) was used to deposit ZrO 2 and silver (Ag)-ZrO 2 composite coatings onto 316L stainless steel (316L SS). Phase purity, surface roughness and surface morphology, thickness of the coatings and elemental compositions of the coatings were analyzed using X-ray diffraction (XRD), atomic force microscopy (AFM) and scanning electron microscopy (SEM) with energy dispersive X-ray spectroscopy (EDS). Total viable count (TVC) and epifluorescence microscopy analysis were studied to evaluate antimicrobial efficiency of ZrO 2 and Ag–ZrO 2 composite coatings using gram negative (gram −ve) Escherichia coli ( E.coli ) and gram positive (gram +ve) Staphylococcus aureus ( S.aureus ). On the basis of the present study, it could be speculated that ZrO 2 coatings exhibited antibacterial activity against only E.coli , whereas Ag–ZrO 2 composite coatings showed superior activity against E.coli and S.aureus strains.

Keywords

  • Zirconia,
  • Silver,
  • Biomedical implants,
  • Thin films,
  • PLD,
  • Antibacterial effect
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References

  1. Arisara et al. (2012) Apatite deposition on ZrO2 thin films by DC unbalanced magnetron sputtering (pp. 45-48) https://doi.org/10.4236/ampc.2012.24B013
  2. Atiyeh et al. (2007) Effect of silver on burn wound infection control and healing: review of the literature (pp. 139-148) https://doi.org/10.1016/j.burns.2006.06.010
  3. Bragg and Rainnie (1974) The effect of silver ions on the respiratory chains of Escherichia coli (pp. 83-889) https://doi.org/10.1139/m74-135
  4. Chen et al. (2007) Antibacterial and osteogenic properties of silver-containing hydroxyapatite coatings produced using a sol gel process (pp. 899-906) https://doi.org/10.1002/jbm.a.31197
  5. Chevalier (2006) What future for zirconia as a biomaterial? (pp. 535-543) https://doi.org/10.1016/j.biomaterials.2005.07.034
  6. Darouiche (1999) Anti-infective efficacy of silver-coated medical prostheses (pp. 1371-1377) https://doi.org/10.1086/313561
  7. Ewald et al. (2006) Antimicrobial titanium/silver PVD coatings on titanium (pp. 1-10) https://doi.org/10.1186/1475-925X-5-22
  8. Gu et al. (2007) Targeted nanoparticles for cancer therapy (pp. 14-21) https://doi.org/10.1016/S1748-0132(07)70083-X
  9. Holleck (1991) Designing advanced coatings for wear protection 7(2) (pp. 137-144) https://doi.org/10.1179/sur.1991.7.2.137
  10. Jangra et al. (2012) Antimicrobial activity of zirconia (ZrO2) nanoparticles and zirconium complexes 12(7105–7)
  11. Jelinek et al. (2013) Antibacterial, cytotoxicity and physical properties of laser—Silver doped hydroxyapatite layers (pp. 1242-1246) https://doi.org/10.1016/j.msec.2012.12.018
  12. Kokubo (2008) Wood-head https://doi.org/10.1533/9781845694227
  13. Lansdown (2006) Silver in health care: antimicrobial effects and safety in use (pp. 17-34) https://doi.org/10.1159/000093928
  14. Lansdown (2007) Critical observations on the neurotoxicity of silver (pp. 237-250) https://doi.org/10.1080/10408440601177665
  15. McDonnell and Russell (1999) Antiseptics and disinfectants: activity, action, and resistance (pp. 147-179)
  16. Nathan et al. (2012) Antibacterial effects of silver-doped hydroxyapatite thin films sputter deposited on titanium (pp. 2135-2144) https://doi.org/10.1016/j.msec.2012.05.012
  17. Sondi (2004) Silver nanoparticles as antimicrobial agent: a case study on E. coli as a model for Gram-negative bacteria (pp. 177-182) https://doi.org/10.1016/j.jcis.2004.02.012
  18. Subramanian and Jayachandran (2007) Characterization of reactive magnetron sputtered nanocrystalline titanium nitride (TiN) thin films with brush plated Ni interlayer (pp. 1069-1075) https://doi.org/10.1007/s10800-007-9357-6
  19. Sun et al. (2008) Preparation and antibacterial activity of Ag–Tio2 composite film by liquid phase deposition (LPD) method (pp. 61-66) https://doi.org/10.1007/s12034-008-0011-7
  20. Suryanarayan and Norton (1998) Plenum Press https://doi.org/10.1007/978-1-4899-0148-4
  21. Thomas et al. (2010) The bacterial cell envelope (pp. 1-16)
  22. Uchida et al. (2002) Structural dependence of apatite formation on zirconia gels in a simulated body fluid (pp. 710-715) https://doi.org/10.2109/jcersj.110.710
  23. Udo (2009) Pathogenic organisms in hip joint infections (pp. 234-240)
  24. Vishwakarma et al. (2009) Antibacterial copper-nickel bilayers and multilayer coatings by pulsed laser deposition on titanium (pp. 705-710) https://doi.org/10.1080/08927010903132183
  25. Willmott and Huber (2000) Pulsed laser vaporization and deposition (pp. 315-328) https://doi.org/10.1103/RevModPhys.72.315
  26. Zheng et al. (2009) Antibacterial property and biocompatibility of plasma sprayed hydroxyapatite/silver composite coatings https://doi.org/10.1007/s11666-009-9376-4