10.1007/s40204-014-0026-7

Bone tissue engineering scaffolding: computer-aided scaffolding techniques

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  • Boonlom Thavornyutikarn1,
  • Nattapon Chantarapanich2,
  • Kriskrai Sitthiseripratip3,
  • George A. Thouas1,
  • Qizhi Chen*1,
  1. Department of Materials Engineering, Monash University, Clayton, VIC, 3800, AU
  2. Department of Mechanical Engineering, Faculty of Engineering at Si Racha, Kasetsart University, Si Racha, Chonburi, 20230, TH
  3. National Metal and Materials Technology Center (MTEC), Klong Luang, Pathumthani, 12120, TH
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Published in Issue 2014-07-17

How to Cite

Thavornyutikarn, B., Chantarapanich, N., Sitthiseripratip, K., Thouas, G. A., & Chen, Q. (2014). Bone tissue engineering scaffolding: computer-aided scaffolding techniques. Progress in Biomaterials, 3(2-4 (December 2014). https://doi.org/10.1007/s40204-014-0026-7
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Abstract

Abstract Tissue engineering is essentially a technique for imitating nature. Natural tissues consist of three components: cells, signalling systems (e.g. growth factors) and extracellular matrix (ECM). The ECM forms a scaffold for its cells. Hence, the engineered tissue construct is an artificial scaffold populated with living cells and signalling molecules. A huge effort has been invested in bone tissue engineering, in which a highly porous scaffold plays a critical role in guiding bone and vascular tissue growth and regeneration in three dimensions. In the last two decades, numerous scaffolding techniques have been developed to fabricate highly interconnective, porous scaffolds for bone tissue engineering applications. This review provides an update on the progress of foaming technology of biomaterials, with a special attention being focused on computer-aided manufacturing (Andrade et al. 2002 ) techniques. This article starts with a brief introduction of tissue engineering ( Bone tissue engineering and scaffolds ) and scaffolding materials ( Biomaterials used in bone tissue engineering ). After a brief reviews on conventional scaffolding techniques ( Conventional scaffolding techniques ), a number of CAM techniques are reviewed in great detail. For each technique, the structure and mechanical integrity of fabricated scaffolds are discussed in detail. Finally, the advantaged and disadvantage of these techniques are compared ( Comparison of scaffolding techniques ) and summarised ( Summary ).

Keywords

  • Computer-aided scaffolding techniques,
  • Solid free-form fabrication,
  • Bioceramics,
  • Bone tissue engineering,
  • Scaffold
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References

  1. Chen A, Tsang V, Albrecht D, Bhatia S (2007) 3-D fabrication technology for tissue engineering. In: Ferrari M, Desai T, Bhatia S (eds) BioMEMS and biomedical nanotechnology. Springer, Berlin, pp 23–38
  2. Oliveira AL, Costa SA, Sousa RA, Reis RL (2009) Nucleation and growth of biomimetic apatite layers on 3D plotted biodegradable polymeric scaffolds: effect of static and dynamic coating conditions. Acta Biomaterialia 5:1626–1638
  3. Lode A, Meissner K, Luo Y, Sonntag F, Glorius S, Nies B, Vater C, Despang F, Hanke T, Gelinsky M (2012) Fabrication of porous scaffolds by three-dimensional plotting of a pasty calcium phosphate bone cement under mild conditions. J Tissue Eng Regenerat Med
  4. Martins et al. (2009) Hierarchical starch-based fibrous scaffold for bone tissue engineering applications (pp. 37-42)
  5. Duan and Wang (2010) Customized CaP/PHBV nanocomposite scaffolds for bone tissue engineering: design, fabrication, surface modification and sustained release of growth factor (pp. S615-S629)
  6. Duan et al. (2010) Three-dimensional nanocomposite scaffolds fabricated via selective laser sintering for bone tissue engineering (pp. 4495-4505)
  7. Sundback et al. (2010) Biocompatibility analysis of poly(glycerol sebacate) as a nerve guide material (pp. 5454-5464)
  8. Vacanti CA (2000) Foreword. In: Lanza RP, Langer R, Vacanti JP (eds) Principles of tissue engineering, 2 edn. Academic Press, California, p xxix
  9. Vacanti CA, Bonassar LJ, Vacanti JP (2000) Structure tissue engineering. In: Lanza RP, Langer R, Vacanti JP (eds) Principles of tissue engineering, 2 edn. Academic Press, California, pp 671–682
  10. Brown CD, Hoffman AS (2002) Modification of natural polymer: Chitosan. In: Atala A, Lanza RP (eds) Methods of tissue engineering. Academic Press, California, pp 565–574
  11. Mota C, Puppi D, Chiellini F, Chiellini E (2012) Additive manufacturing techniques for the production of tissue engineering constructs. J Tissue Eng Regenerat Med
  12. Lam CXF, Olkowski R, Swieszkowski W, Tan KC, Gibson I, Hutmacher DW (2009) Composite PLDLLA/TCP scaffolds for bone engineering: mechanical and in vitro evaluations. In: The 13th international conference on biomedical engineering, Singapore, pp 1480–1483
  13. Mooney et al. (1996) Novel approach to fabricate porous sponges of poly(d,l-lactic-co-glycolic acid) without the use of organic solvents (pp. 1417-1422)
  14. Stuckey et al. (2010) Magnetic resonance imaging evaluation of remodeling by cardiac elastomeric tissue scaffold biomaterials in a rat model of myocardial infarction (pp. 3395-3402)
  15. Hutmacher DW, Hoque ME, Wong YS (2008) Design, fabrication and physical characterization of scaffolds made from biodegradable synthetic polymers in combination with RP systems based on melt extrusion. In: Bidanda B, Bártolo PJ (eds) Virtual prototyping & bio manufacturing in medical applications. Springer, New York, pp 261–291
  16. Melchels et al. (2010) Effects of the architecture of tissue engineering scaffolds on cell seeding and culturing (pp. 4208-4217)
  17. Kim and Oh (2008) A benchmark study on rapid prototyping processes and machines: quantitative comparisons of mechanical properties, accuracy, roughness, speed, and material cost (pp. 201-215)
  18. Lee G, Barlow JW (1993) Selective laser sintering of bioceramic materials for implants. In: Proceedings of solid freeform fabrication symposium, Austin, TX, pp 376–380
  19. Tartarisco et al. (2009) Polyurethane unimorph bender microfabricated with Pressure Assisted Microsyringe (PAM) for biomedical applications (pp. 1835-1841)
  20. Vozzi G, Tirella A, Ahluwalia A (2012) Rapid prototyping composite and complex scaffolds with PAM2. Methods Mol Biol (Clifton, N.J.) 868:57–69
  21. Cornejo IA, McNulty TF, Lee SY, Bianchi E, Danforth SC, Safari A (2000) Development of bioceramic tissue scaffolds via fused deposition of ceramics. In: George L (ed) Bioceramics: materials and applications III. American Ceramic Society, Westerville, pp 183–195
  22. Reichert JC, Hutmacher DW (2011) Bone tissue engineering. In:
  23. Tissue Engineering
  24. , N. Pallua and C. V. Suschek, Eds., ed New York: Springer, 2011, pp. 431-456
  25. Cesarano et al. (1998) Robocasting provides moldless fabrication from slurry deposition (pp. 94-102)
  26. Smay JE, Lewis JA (2012) Solid free-form fabrication of 3-D ceramic structures. In: Bansal NP, Boccaccini AR (eds) Ceramics and composites processing methods, 1st edn. Wiley, New Jersey, pp 459–484
  27. Jansen J, Melchels FPW, Grijpma DW, Feijen J (2008) Fumaric acid monoethyl ester-functionalized poly(
  28. D,L
  29. -lactide)/
  30. N
  31. -vinyl-2-pyrrolidone resins for the preparation of tissue engineering scaffolds by stereolithography. Biomacromolecules 10:214–220
  32. Stuecker et al. (2003) Control of the viscous behavior of highly concentrated mullite suspensions for robocasting (pp. 318-325)
  33. Temenoff JS, Lu L, Mikos AG (2000) Bone tissue engineering using synthetic biodegradable polymer scaffolds. In: Davies JE (ed) Bone engineering. EM Squared, Toronto, pp 455–462
  34. Swift KG, Booker JD (2013) Rapid prototyping processes. In: Manufacturing process selection handbook. Butterworth-Heinemann, Oxford, pp 227–241
  35. Schwartzalder K, Somers AV (1963) Method of making a porous shape of sintered refractory ceramic articles
  36. Hench LL (1999) Bioactive glasses and glasses–ceramics. In: Shackelford JF (ed) Biocaramics-applications of ceramic and glass materials in medicine. Trans Tech Publication, Switzerland, pp 37–64
  37. Ye L, Zeng X, Li H, Ai Y (2010) Fabrication and biocompatibility of nano non-stoichiometric apatite and poly(ε-caprolactone) composite scaffold by using prototyping controlled process. J Mater Sci Mater Med 21:753–760
  38. Murphy MB, Mikos AG (2007) Polymer scaffold fabrication. In: Lanza R, Langer R, Vacanti J (eds) Principles of tissue engineering, 3 edn. Academic Press, California, pp 309–321
  39. O’Donnell MD (2012) Melt-derived bioactive glass. In: Jones JR, Clare AG (eds) Bio-glasses: an introduction. Wiley, West Sussex
  40. Gurr M, Mülhaupt R (2012) Rapid prototyping. In: Krzysztof M, Martin M (eds) Polymer science: a comprehensive reference. Elsevier, Amsterdam, pp 77–99
  41. Potoczek et al. (2009) Manufacturing of highly porous calcium phosphate bioceramics via gel-casting using agarose (pp. 2249-2254)
  42. Hopkinson N, Dickens P (2006) Emerging rapid manufacturing processes. In: Hopkinson N, Hague RJM, Dickens PM (eds) Rapid manufacturing: an industrial revolution for the digital age. Wiley, West Sussex, pp 55–80
  43. Dalton et al. (2009) Snapshot: polymer scaffolds for tissue engineering (pp. 701-702)
  44. Bartolo PJ, Almeida HA, Rezende RA, Laoui T, Bidanda B (2008) Advanced processes to fabricate scaffolds for tissue engineering. In: Bidanda B, Bartolo PJ (eds) Virtual prototyping of biomanufacturing in medical application. Springer, New York, pp 149–170
  45. Wolfe PS, Sell SA, Bowlin GL (2011) Natural and synthetic scaffolds. In: Pallua N, Suschek CV (eds) Tissue engineering. Springer, New York, pp 41–67
  46. Ma PX, Langer R (1995) Degradation, structure and properties of fibrous poly(glycolic acid) scaffolds for tissue engineering. In: Mikos AG (ed) Polymers in medicine and pharmacy, vol 394. Materials Research Society, Pennsylvania, pp 99–110
  47. Chen Q, Liang S, Thouas GA (2013) Elastomeric biomaterials for tissue engineering. Progress in polymer science, vol 38, pp 584–671
  48. Fu et al. (2011) Direct ink writing of highly porous and strong glass scaffolds for load-bearing bone defects repair and regeneration (pp. 3547-3554)
  49. Chen QZ (2007) Bioglass
  50. ®
  51. -derived glass-ceramic scaffolds for bone tissue engineering. PhD, Materials, Imperial College London, London
  52. Chen QZ, Roether JA, Boccaccini AR (2008) Tissue engineering scaffolds from bioactive glass and composite materials. In: Ashammakhi N, Reis R, Chiellini F (eds) Topics in tissue engineering, vol 4. BTE group, pp 1–23
  53. Chen et al. (2008) Characterisation of a soft elastomer poly(glycerol sebacate) designed to match the mechanical properties of myocardial tissue (pp. 47-57)
  54. Chen Q-Z, Harding SE, Ali NN, Lyon AR, Boccaccini AR (2008) Biomaterials in cardiac tissue engineering: ten years of research survey. Mater Sci Eng R Rep 59:1–37
  55. Muzzarelli RAA, Muzzarelli C (2005) Chitosan chemistry: relevance to the biomedical sciences. In: Polysaccharides 1: structure, characterization and use. Springer, Berlin, pp 151–209
  56. Kohn J, Langer R (1996) Bioresorbable and bioerodible materials. In: Ratner BD, Hoffman AS, Schoen FJ, Lemons JE (eds) Biomaterials science: an introduction to materials in medicine. Academic Press, New York, pp 64–72
  57. Danforth S, Safari A, JMA, Langrana N (1998) Solid free form fabrication (SFF) of functional advanced ceramic components. Naval research review, office of naval research three, vol L, pp 27–38
  58. Hollister (2005) Porous scaffold design for tissue engineering (pp. 518-524)
  59. Onagoruwa S, Bose S, Bandyopadhyay A (2001) Fused deposition of ceramics (FDC) and composites. In: Solid freeform fabrication symposium, The University of Texas at Austin, pp 224–231
  60. Padilla S, Sánchez-Salcedo S, Vallet-Regí M (2006) Bioactive glass as precursor of designed-architecture scaffolds for tissue engineering. J Biomed Mater Res 81A:224–232
  61. Liang S-L, Cook WD, Thouas GA, Chen Q-Z (2010) The mechanical characteristics and in vitro biocompatibility of poly(glycerol sebacate)-Bioglass
  62. ®
  63. elastomeric composites. Biomaterials 31:8516–8529
  64. Pereira TF, Oliveira MF, Maia IA, Silva JVL, Costa MF, Thiré RMSM (2012) 3D printing of poly(3-hydroxybutyrate) porous structures using selective laser sintering. Macromolecular Symposia 319:64–73
  65. Grimm T (2004) User’s guide to rapid prototyping. Society of Manufacturing Engineers (SME), Dearborn, pp 367–396
  66. Chu TM, Halloran JW, Wagner WC (1997) Hydroxyapatite suspension for implant fabrication by stereolithography. In: Ghosh A, Barks RE, Hiremath B (eds) Case studies in ceramic product development. American Ceramic Society, Westerville, pp 119–125
  67. Chu TM, Halloran JW, Wagner WC (1996) Ultraviolet curing of highly loaded hydroxyapatite suspension. In: Rusin RP, Fischman GS (eds) Bioceramics: materials and applications II. American Ceramic Society, Westerville, pp 57–66
  68. Niemelä T, Kellomäki M (2011) Bioactive glass and biodegradable polymer composites. In: Ylänen HO (ed) Bioactive glasses: materials, properties and applications. Woodhead Publishing Limited, Cambridge, pp 227–245
  69. Chu TMG (2006) Solid freeform fabrication of tissue engineering scaffolds. In: Ma PX, Elisseeff J (eds) Scaffolding in tissue engineering. CRC Press, Florida, pp 139–153
  70. Popov VK, Antonov EN, Bagratashvili VN, Konovalov AN, Howdle SM (2004) Selective laser sintering of 3-D biodegradable scaffolds for tissue engineering. In: Materials research society symposium proceeding, pp F.5.4.1–F.5.4.3
  71. Correlo VM, Oliveira JM, Mano JF, Neves NM, Reis RL (2011) Natural origin materials for bone tissue engineering e properties, processing, and performance. In: Atala A, Lanza R, Thomson JA, Nerem RM (eds) Principles of regenerative medicine, 2 edn. Academic Press, Canada, pp 557–586
  72. Sun W, Starly B, Nam J, Darling A (2005) Bio-CAD modeling and its applications in computer-aided tissue engineering. Comput-Aided Des 37:1097–1114
  73. Morsi YS, Wong CS, Patel SS (2008) Conventional manufacturing processes for three-dimensional scaffolds. In: Bidanda B, Bartolo PJ (eds) Virtual prototyping of biomanufacturing in medical application. Springer, New York, pp 129–148
  74. Andrade et al. (2002) Behavior of dense and porous hydroxyapatite implants and tissue response in rat femoral defects (pp. 30-36)
  75. Anithaa et al. (2001) Critical parameters in ̄uencing the quality of prototypes in fused deposition modelling (pp. 385-388)
  76. Antonov et al. (2005) Three-dimensional bioactive and biodegradable scaffolds fabricated by surface-selective laser sintering (pp. 327-330)
  77. Arafat et al. (2011) Biomimetic composite coating on rapid prototyped scaffolds for bone tissue engineering (pp. 809-820)
  78. Arcaute et al. (2010) Stereolithography of spatially controlled multi-material bioactive poly(ethylene glycol) scaffolds (pp. 1047-1054)
  79. Attawin et al. (1995) Osteoblast-like cell adherence and migration through 3-dimensional porous polymer matrices (pp. 639-644)
  80. Bael et al. (2013) In vitro cell-biological performance and structural characterization of selective laser sintered and plasma surface functionalized polycaprolactone scaffolds for bone regeneration (pp. 3404-3412)
  81. Baino and Vitale-Brovarone (2011) Three-dimensional glass-derived scaffolds for bone tissue engineering: current trends and forecasts for the future (pp. 514-535)
  82. Barroca et al. (2010) Tailoring the morphology of high molecular weight PLLA scaffolds through bioglass addition (pp. 3611-3620)
  83. Bergmann et al. (2010) 3D printing of bone substitute implants using calcium phosphate and bioactive glasses (pp. 2563-2567)
  84. Bergsma et al. (1993) Foreign-body reactions to resorbable poly(l-lactide) bone plates and screws used for the fixation of unstable zygomatic fractures (pp. 666-670)
  85. Bernardo JR (2010) Indirect tissue scaffold fabrication via additive manufacturing and biomimetic mineralization. Master of Science, Mechanical Engineering, The Virginia Polytechnic Institute and State University, Blacksburg, Virginia
  86. Bettahalli et al. (2013) Corrugated round fibers to improve cell adhesion and proliferation in tissue engineering scaffolds (pp. 6928-6935)
  87. Bettinger (2011) Biodegradable elastomers for tissue engineering and cell–biomaterial interactions (pp. 467-482)
  88. Billiet et al. (2012) A review of trends and limitations in hydrogel-rapid prototyping for tissue engineering (pp. 6020-6041)
  89. Blaker et al. (2003) In vitro evaluation of novel bioactive composites based on Bioglass®-filled polylactide foams for bone tissue engineering scaffolds (pp. 1401-1411)
  90. Blaker et al. (2005) Mechanical properties of highly porous PDLLA/Bioglass® composite foams as scaffolds for bone tissue engineering (pp. 643-652)
  91. Boccaaccini et al. (2003) Bioresorbable and bioactive composite materials based on polylactide foams filled with and coated by Bioglass® particles for tissue engineering applications (pp. 443-450)
  92. Boccaccini and Blaker (2005) Bioactive composite materials for tissue engineering scaffolds (pp. 303-317)
  93. Bose et al. (1999) Processing of controlled porosity ceramic structures via fused deposition (pp. 1009-1014)
  94. Bostman and Pihlajamaki (2000) Adverse tissue reactions to bioabsorbable fixation devices (pp. 216-227)
  95. Bretcanu and Boccaccini (2012) Poly-DL-lactic acid coated Bioglass® scaffolds: toughening effects and osteosarcoma cell proliferation (pp. 5661-5672)
  96. Bretcanu et al. (2007) Biodegradable polymer coated 45S5 Bioglass-derived glass-ceramic scaffolds for bone tissue engineering (pp. 227-234)
  97. Bretcanu et al. (2009) In vitro biocompatibility of 45S5 Bioglass®-derived glass-ceramic scaffolds coated with poly(3-hydroxybutyrate) (pp. 139-148)
  98. Brovarone et al. (2006) Macroporous bioactive glass-ceramic scaffolds for tissue engineering (pp. 1069-1078)
  99. Brovarone et al. (2008) Biocompatible glass–ceramic materials for bone substitution (pp. 471-478)
  100. Brown et al. (2008) Growth and differentiation of osteoblastic cells on 13–93 bioactive glass fibers and scaffolds (pp. 387-396)
  101. Bruder SP, Caplan AI (2000) Bone regeneration through cellular engineering. In: Lanza RP, Lannger R, Vacanti JP (eds) Principles of tissue engineering, 2 edn. Academic Press, California, pp 683–696
  102. Burg et al. (2000) Biomaterial developments for bone tissue engineering (pp. 2347-2359)
  103. Cao and Kuboyama (2010) A biodegradable porous composite scaffold of PGA/β-TCP for bone tissue engineering (pp. 386-395)
  104. Chai et al. (2012) Current views on calcium phosphate osteogenicity and the translation into effective bone regeneration strategies (pp. 3876-3887)
  105. Chen (2011) Foaming technology of tissue engineering scaffolds—a review (pp. 34-47)
  106. Chen and Boccaccini (2006) Poly(d, l-lactic acid) coated 45S5 Bioglass®-based scaffolds: processing and characterization (pp. 445-457)
  107. Chen and Thouas (2011) Fabrication and characterization of sol–gel derived 45S5 Bioglass®–ceramic scaffolds (pp. 3616-3626)
  108. Chen and Wu (2005) The application of polyhydroxyalkanoates as tissue engineering materials (pp. 6565-6578)
  109. Chen et al. (2006) 45S5 Bioglass®-derived glass-ceramic scaffolds for bone tissue engineering (pp. 2414-2425)
  110. Chen et al. (2008) Bioglass®-derived glass–ceramic scaffolds: study of cell proliferation and scaffold degradation in vitro (pp. 1049-1060)
  111. Chen et al. (2010) A new sol–gel process for producing Na2O-containing bioactive glass ceramics (pp. 4143-4153)
  112. Chen et al. (2012) Progress and challenges in biomaterials for tissue engineering
  113. Chen et al. (2012) Progress and challenges in biomaterials used for bone tissue engineering: bioactive glasses and elastomeric composites (pp. 1-22)
  114. Chen et al. (2012) Spark plasma sintering of sol–gel derived 45S5 Bioglass®-ceramics: mechanical properties and biocompatibility evaluation (pp. 494-502)
  115. Choi et al. (2009) Fabrication of 3D biocompatible/biodegradable micro-scaffolds using dynamic mask projection microstereolithography (pp. 5494-5503)
  116. Chu et al. (2002) Mechanical and in vivo performance of hydroxyapatite implants with controlled architectures (pp. 1283-1293)
  117. Chua CK, Leong KF, Sudarmadji N, Liu MJJ, Chou SM (2011) Selective laser sintering of functionally graded tissue scaffolds, Matrials Research Society, vol 36, pp 1006–1014
  118. Cooke et al. (2002) Use of stereolithography to manufacture critical-sized 3D biodegradable scaffolds for bone ingrowth (pp. 65-69)
  119. Crouch et al. (2009) Correlation of anisotropic cell behaviors with topographic aspect ratio (pp. 1560-1567)
  120. Cruz F, Simoes J, Coole T (2005) Direct manufacture of hydroxyapatite based bone implants by selective laser sintering. In: 2nd international conference on advanced research in virtual rapid protrotyping, Leiria, Portugal, p 119
  121. Daoud et al. (2011) Long-term in vitro human pancreatic islet culture using three-dimensional microfabricated scaffolds (pp. 1536-1542)
  122. Dawson et al. (2008) Development of specific collagen scaffolds to support the osteogenic and chondrogenic differentiation of human bone marrow stromal cells (pp. 3105-3116)
  123. Day et al. (2004) Assessment of polyglycolic acid mesh and bioactive glass for soft-tissue engineering scaffolds (pp. 5857-5866)
  124. Dellinger et al. (2006) Robotic deposition of model hydroxyapatite scaffolds with multi architectures and multiscale porosity for bone tissue engineering (pp. 383-394)
  125. Devin et al. (1996) Three-dimensional degradable porous polymer-ceramic matrices for use in bone repair (pp. 661-669)
  126. Dhandayuthapani et al. (2011) Polymeric scaffolds in tissue engineering application: a review (pp. 1-19)
  127. Doi et al. (1995) Microbial synthesis and characterization of poly(3-hydroxyburyrate-co-3-hydroxyhexanoate) (pp. 4822-4828)
  128. Doyle et al. (1991) In vitro and in vivo evaluation of polyhydroxyburyrate and polyhydroxybutyrate reinforced with hydroxyapatite (pp. 841-847)
  129. Duan and Wang (2010) Encapsulation and release of biomolecules from CaP/PHBV nanocomposite microspheres and three-dimensional scaffolds fabricated by selective laser sintering (pp. 1655-1664)
  130. Elomaa et al. (2011) Preparation of poly(ε-caprolactone)-based tissue engineering scaffolds by stereolithography (pp. 3850-3856)
  131. Elomaa et al. (2013) Porous 3D modeled scaffolds of bioactive glass and photocrosslinkable poly(ε-caprolactone) by stereolithography (pp. 99-106)
  132. Eosoly et al. (2010) Selective laser sintering of hydroxyapatite/poly-ε-caprolactone scaffolds (pp. 2511-2517)
  133. Eosoly et al. (2012) Interaction of cell culture with composite effects on the mechanical properties of polycaprolactone-hydroxypatite scaffolds fabricated via selective laser sintering (SLS) (pp. 2250-2257)
  134. Eshraghi and Das (2010) Mechanical and microstructural properties of polycaprolactone scaffolds with one-dimensional, two-dimensional, and three-dimensional orthogonally oriented porous architectures produced by selective laser sintering (pp. 2467-2476)
  135. Eslaminejad et al. (2007) Bone differentiation of marrow-derived mesenchymal stem cells using β-tricalcium phosphate–alginate–gelatin hybrid scaffolds (pp. 417-424)
  136. Felzmann et al. (2012) Lithography-based additive manufacturing of cullular ceramic structures (pp. 1052-1058)
  137. Ferreira et al. (2012) Collagen for bone tissue regeneration (pp. 3191-3200)
  138. Fielding et al. (2012) Effects of silica and zinc oxide doping on mechanical and biological properties of 3D printed tricalcium phosphate tissue engineering scaffolds (pp. 113-122)
  139. Fierz et al. (2008) The morphology of anisotropic 3D-printed hydroxyapatite scaffolds (pp. 3799-3806)
  140. Fisher et al. (2001) Photoinitiated polymerization of biomaterials (pp. 171-181)
  141. Fu et al. (2008) Mechanical and in vitro performance of 13–93 bioactive glass scaffolds prepared by a polymer foam replication technique (pp. 1854-1864)
  142. Fu et al. (2011) Bioactive glass scaffolds for bone tissue engineering: state of the art and future perspectives (pp. 1245-1256)
  143. Fukasawa et al. (2001) Pore structure of porous ceramics synthesized from water-based slurry by freeze-dry process (pp. 2523-2527)
  144. Ge et al. (2009) Proliferation and differentiation of human osteoblasts within 3D printed poly-lactic-co-glycolic acid scaffolds (pp. 533-547)
  145. Gerhardt and Boccaccini (2010) Bioactive glass and glass-ceramic scaffolds for bone tissue engineering (pp. 3867-3910)
  146. Gollwitzer et al. (2003) Antibacterial poly(d, l-lactic acid) coating of medical implants using a biodegradable drug delivery technology (pp. 585-591)
  147. Gollwitzer et al. (2005) Biomechanical and allergological characteristics of a biodegradable poly(d, l-lactic acid) coating for orthopaedic implants (pp. 802-809)
  148. Goodridge RD (2004) Indirect selective laser sintering of an apatite-mullite glass–ceramic. PhD School of Mechanical Engineering, University of Leeds
  149. Goodridge et al. (2007) Biological evaluation of an apatite–mullite glass-ceramic produced via selective laser sintering (pp. 221-231)
  150. Guan and Davies (2004) Preparation and characterization of a highly macroporous biodegradable composite tissue engineering scaffold (pp. 480-487)
  151. Harris et al. (1998) Open pore biodegradable matrices formed with gas foaming (pp. 396-402)
  152. Hattiangadi and Bandyopadhyay (2000) Modeling of multiple pore ceramic materials fabricated via fused deposition process (pp. 581-588)
  153. Haugen et al. (2004) Ceramic TiO2-foams: characterisation of a potential scaffold (pp. 661-668)
  154. Hayati et al. (2011) Preparation of poly(3-hydroxybutyrate)/nano-hydroxyapatite composite scaffolds for bone tissue engineering (pp. 736-739)
  155. Heller et al. (2009) Vinyl esters: low cytotoxicity monomers for the fabrication of biocompatible 3D scaffolds by lithography based additive manufacturing (pp. 6941-6954)
  156. Hench (1998) Bioceramics (pp. 1705-1728)
  157. Hench (2006) The story of Bioglass® (pp. 967-978)
  158. Hench and Wilson (1999) Word Scientific
  159. Hench et al. (1971) Bonding mechanisms at the interface of ceramic prosthetic materials (pp. 117-141)
  160. Heo et al. (2009) Fabrication and characterization of novel nano-and micro-HA/PCL composite scaffolds using a modified rapid prototyping process (pp. 108-116)
  161. Hoelzle et al. (2008) Micro-robotic deposition guidelines by a design of experiments approach to maximize fabrication reliability for the bone scaffold application (pp. 897-912)
  162. Hoque et al. (2011) Extrusion based rapid prototyping technique: an advanced platform for tissue engineering scaffold fabrication (pp. 83-93)
  163. Hsu et al. (2007) Evaluation of the growth of chondrocytes and osteoblasts seeded into precision scaffolds fabricated by fused deposition manufacturing (pp. 519-527)
  164. Hutmacher (2000) Scaffolds in tissue engineering bone and cartilage (pp. 2529-2543)
  165. Hutmacher and Cool (2007) Concepts of scaffold-based tissue engineering-the rationale to use solid free-form fabrication techniques (pp. 654-669)
  166. Hutmacher et al. (2001) Mechanical properties and cell cultural response of polycaprolactone scaffolds designed and fabricated via fused deposition modeling (pp. 203-216)
  167. Hutmacher et al. (2004) Scaffold-based tissue engineering: rationale for computer-aided design and solid free-form fabrication systems (pp. 354-362)
  168. Ishizaki et al. (1998) Kluwer Academic Publisher
  169. Iyer et al. (2008) Microstructural characterization and mechanical properties of Si3N4 fomed by fused deposition of ceramics (pp. 127-137)
  170. Jones (2013) Review of bioactive glass: from Hench to hybrids (pp. 4457-4486)
  171. Kalita et al. (2003) Development of controlled porosity polymer–ceramic composite scaffolds via fused deposition modeling (pp. 611-620)
  172. Kanczler et al. (2009) Biocompatibility and osteogenic potential of human fetal femur-derived cells on surface selective laser sintered scaffolds (pp. 2063-2071)
  173. Kemppainen and Hollister (2010) Tailoring the mechanical properties of 3D-designed poly(glycerol sebacate) scaffolds for cartilage applications (pp. 9-18)
  174. Khoda AKMB, Ozbolat IT, Koc B (2010) Engineered tissue scaffolds with variational porous architecture. J Biomech Eng 133:011001
  175. Kim and Cho (2009) The optimization of hybrid scaffold fabrication process in precision deposition system using design of experiments (pp. 843-851)
  176. Kim and Cho (2009) Blended PCL/PLGA scaffold fabrication using multi-head deposition system (pp. 1447-1450)
  177. Kim et al. (1998) Survival and function of hepatocytes on a novel three-dimensional synthetic biodegradable polymer scaffold with an intrinsic network of channels (pp. 8-13)
  178. Kim et al. (2003) Porous ZrO2 bone scaffold coated with hydroxyapatite with fluorapatite intermediate layer (pp. 3277-3284)
  179. Kim et al. (2004) Effect of PEG–PLLA diblock copolymer on macroporous PLLA scaffoldsbythermallyinducedphaseseparation (pp. 2319-2329)
  180. Kim et al. (2010) Evaluation of solid free-form fabrication-based scaffolds seeded with osteoblasts and human umbilical vein endothelial cells for use in vivo osteogenesis (pp. 2229-2236)
  181. Kolan et al. (2012) Effect of material, process parameters, and simulated body fluids on mechanical properties of 13-93 bioactive glass porous constructs made by selective laser sintering (pp. 14-24)
  182. Korpela et al. (2013) Biodegradable and bioactive porous scaffold structures prepared using fused deposition modeling (pp. 610-619)
  183. Kruth et al. (2003) Lasers and materials in selective laser sintering (pp. 357-371)
  184. Lam et al. (2009) Evaluation of polycaprolactone scaffold degradation for 6 months in vitro and in vivo (pp. 906-919)
  185. Landers et al. (2002) Fabrication of soft tissue engineering scaffolds by means of rapid prototyping techniques (pp. 3107-3116)
  186. Landers et al. (2002) Rapid prototyping of scaffolds derived from thermoreversible hydrogels and tailored for applications in tissue engineering (pp. 4437-4447)
  187. Langer and Vacanti (1993) Tissue engineering (pp. 920-927)
  188. Langer et al. (1995) Tissue engineering biomedical applications (pp. 151-161)
  189. Laurencin et al. (1996) Tissue engineered bone-regeneration using degradable polymers: the formation of mineralized matrices (pp. S93-S99)
  190. Lee et al. (2007) Poly(propylene fumalate) bone tissue engineering scaffold fabrication using stereolithography: effects of resin formulations and laser parameters (pp. 1077-1084)
  191. Lee et al. (2008) Fabrication and characteristic analysis of a poly(propylene fumate) scaffold using micro-stereolithography technology (pp. 1-9)
  192. Lee et al. (2008) Advances in 3D nano/microfabrication using two-photon initiated polymerization (pp. 631-681)
  193. Lee et al. (2012) Effect of pore architecture and stacking direction on mechanical properties of solid freeform fabrication-based scaffold for bone tissue engineering (pp. 1846-1853)
  194. Leong et al. (2003) Solid freeform fabrication of three-dimensional scaffolds for engineering replacement tissues and organs (pp. 2363-2378)
  195. Leong et al. (2007) Absolute quantification of gene expression in biomaterials research using real-time PCR (pp. 203-210)
  196. Li and Chang (2004) Preparation and characterization of bioactive and biodegradable Wollastonite/poly(d, l-lactic acid) composite scaffolds (pp. 1089-1095)
  197. Li et al. (2005) Fabrication, characterization, and in vitro degradation of composite scaffolds based on PHBV and bioactive glass (pp. 137-155)
  198. Li et al. (2011) Fabrication of individual scaffolds based on a patient-specific alveolar bone defect model (pp. 87-93)
  199. Li et al. (2013) Synthesis, characterization and properties of biocompatible poly(glycerol sebacate) pre-polymer and gel (pp. 534-547)
  200. Li et al. (2013) Stiff macro-porous bioactive glasse ceramic scaffold: fabrication by rapid prototyping template, characterization and in vitro bioactivity (pp. 76-80)
  201. Liu et al. (2009) Bioactive borosilicate glass scaffolds: improvement on the strength of glass-based scaffolds for tissue engineering (pp. 365-372)
  202. Liu et al. (2009) Multinozzle low-temperature deposition system for construction of gradient tissue engineering scaffolds (pp. 254-263)
  203. Liu et al. (2013) Review: development of clinically relevant scaffolds for vascularised bone tissue engineering (pp. 688-705)
  204. Lohfeld et al. (2012) Fabrication, mechanical and in vivo performance of polycaprolactone/tricalcium phosphate composite scaffolds (pp. 3446-3456)
  205. Lorrison et al. (2005) Processing of an apatite-mullite glass-ceramic and an hydroxyapatite/phosphate glass composite by selective laser sintering (pp. 775-781)
  206. Lu et al. (2003) Three-dimensional, bioactive, biodegradable, polymer-bioactive glass composite scaffolds with improved mechanical properties support collagen synthesis and mineralization of human osteoblast-like cells in vitro (pp. 465-474)
  207. Mano et al. (2004) Bioinert, biodegradable and injectable polymeric matrix composites for hard tissue replacement: state of the art and recent developments (pp. 789-817)
  208. Maquet et al. (2003) Preparation, characterization, and in vitro degradation of bioresorbable and bioactive composites based on Bioglass®-filled polylactide foams (pp. 335-346)
  209. Maquet et al. (2004) Porous poly(alpha-hydroxyacid)/Bioglass® composite scaffolds for bone tissue engineering. I: preparation and in vitro characterisation (pp. 4185-4194)
  210. Marcacci et al. (2007) Stem cells associated with macroporous bioceramics for long bone repair: 6- to 7-year outcome of a pilot clinical study (pp. 947-955)
  211. Martin et al. (1996) Acidity near eroding polylactide–polyglycolide in vitro and in vivo rabbit tibial bone chambers (pp. 2373-2380)
  212. Martínez-Vázquez et al. (2010) Improving the compressive strength of bioceramic robocast scaffolds by polymer infiltration (pp. 4361-4368)
  213. Melchels et al. (2009) A poly(d, l-lactide) resin for the preparation of tissue engineering scaffolds by stereolithography (pp. 3801-3809)
  214. Melchels et al. (2010) A review on stereolithography and its applications in biomedical engineering (pp. 6121-6130)
  215. Melchels et al. (2012) Additive manufacturing of tissues and organs (pp. 1079-1104)
  216. Meszaros et al. (2011) Three-dimensional printing of a bioactive glass (pp. 111-116)
  217. Metze et al. (2013) Gelatin coated 45S5 Bioglass®-derived scaffolds for bone tissue engineering (pp. 31-39)
  218. Middleton and Tipton (2000) Synthetic biodegradable polymers as orthopedic devices (pp. 2335-2346)
  219. Mikos and Temenoff (2000) Formation of highly porous biodegradable scaffolds for tissue engineering (pp. 1-6)
  220. Miranda et al. (2006) Sintering and robocasting of β-tricalcium phosphate scaffolds for orthopaedic applications (pp. 457-466)
  221. Miranda et al. (2008) Finite element modeling as a tool for predicting the fracture behavior of robocast scaffolds (pp. 1715-1724)
  222. Misra et al. (2006) Polyhydroxyalkanoate (PHA)/inorganic phase composites for tissue engineering applications (pp. 2249-2258)
  223. Molladavoodi et al. (2013) Investigation of microstructure, mechanical properties and cellular viability of poly(L-lactic acid) tissue engineering scaffolds prepared by different thermally induced phase separation protocols (pp. 186-197)
  224. Montanaro et al. (1998) Ceramic foams by powder processing (pp. 1339-1350)
  225. Muzzarelli et al. (1993) Osteoconductive properties of methlpyrrolidinone chitosan in an animal-model (pp. 925-929)
  226. Nam and Park (1999) Biodegradable polymeric microcellular foams by modified thermally induced phase separation method (pp. 1783-1790)
  227. Navarro et al. (2004) Development and cell response of a new biodegradable composite scaffold for guided bone regeneration (pp. 419-422)
  228. Oliveira et al. (2010) Peripheral mineralization of a 3D biodegradable tubular construct as a way to enhance guidance stabilization in spinal cord injury regeneration (pp. 2821-2830)
  229. Pham et al. (2006) Electrospinning of polymeric nanofibers for tissue engineering applications: a review (pp. 1197-1211)
  230. Pham et al. (2008) Deterioration of polyamide powder properties in the laser sintering process. Proceedings of The Institution of Mechanical Engineers, Part C (pp. 2163-2176)
  231. Pitt et al. (1981) Aliphatic polyesters. 2. The degradation of poly(DL-lactide), poly(ε-caprolactone) and their copolymers in vivo (pp. 215-220)
  232. Potijanyakul et al. (2010) Effects of enamel matrix derivative on bioactive glass in rat calvarium defects (pp. 195-204)
  233. Puppi et al. (2010) Polymeric materials for bone and cartilage repair (pp. 403-440)
  234. Ramanath et al. (2008) Melt flow behaviour of poly-ε-caprolactone in fused deposition modeling (pp. 2541-2550)
  235. Ramay and Zhang (2003) Preparation of porous hydroxyapatite scaffolds by combination of the gel-casting and polymer sponge methods (pp. 3293-3302)
  236. Rattanakit et al. (2012) Extrusion printed polymer structures: a facile and versatile approach to tailored drug delivery platforms (pp. 254-263)
  237. Raucci et al. (2010) Hybrid composite scaffolds prepared by sol–gel method for bone regeneration (pp. 1861-1868)
  238. Reed (1988) Wiley
  239. Rezwan et al. (2006) Biodegradable and bioactive porous polymer/inorganic composite scaffolds for bone tissue engineering (pp. 3413-3431)
  240. Rich et al. (2002) In vitro evaluation of poly(ε-caprolactone-co-d,l-lactide)/bioactive glass composites (pp. 2143-2150)
  241. Roether et al. (2002) Novel bioresorbable and bioactive composites based on bioactive glass and polylactide foams for bone tissue engineering (pp. 1207-1214)
  242. Roy et al. (2003) Performance of hydroxyapatite bone repair scaffolds created via three-dimensional fabrication techniques (pp. 1228-1237)
  243. Russell and Block (1999) Clinical utility of demineralized bone matrix for osseous defects, arthrodesis, and reconstruction: impact of processing techniques and study methodology (pp. 524-531)
  244. Russias et al. (2007) Fabrication and in vitro characterization of three-dimensional organic/inorganic scaffolds by robocasting (pp. 434-445)
  245. Santos et al. (2012) Design and production of sintered β-tricalcium phosphate 3D scaffolds for bone tissue regeneration (pp. 1293-1298)
  246. Schmidmaier et al. (2001) Local application of growth factors (insulin-like growth factor-1 and transforming growth factor-beta 1) from a biodegradable poly(d,l-lactide) coating of osteosynthetic implants accelerates fracture healing in rats (pp. 341-350)
  247. Schmidmaier et al. (2001) Biodegradable poly(d, l-lactide) coating of implants for continuous release of growth factors (pp. 449-455)
  248. Seck et al. (2010) Designed biodegradable hydrogel structures prepared by stereolithography using poly(ethylene glycol)/poly(d, l-lactide)-based resins (pp. 34-41)
  249. Seitz et al. (2005) Three-dimensional printing of porous ceramic scaffolds for bone tissue engineering (pp. 782-788)
  250. Seol et al. (2013) A new method of fabricating robust freeform 3D ceramic scaffolds for bone tissue regeneration (pp. 1444-1455)
  251. Seppala et al. (2011) Photocrosslinkable polyesters and poly(ester anhydride)s for biomedical applications (pp. 1647-1652)
  252. Sepulveda et al. (2002) Bioactive sol–gel foams for tissue repair (pp. 340-348)
  253. Serra et al. (2013) High-resolution PLA-based composite scaffolds via 3-D printing technology (pp. 5521-5530)
  254. Shanjani et al. (2011) Mechanical characteristics of solid-freeform-fabricated porous calcium polyphosphate structures with oriented stacked layers (pp. 1788-1796)
  255. Sharaf et al. (2012) Three-dimensionally printed polycaprolactone and β-tricalcium phosphate scaffolds for bone tissue engineering: an in vitro study (pp. 647-656)
  256. Sharifi et al. (2011) Preparation, mechanical properties, and in vitro biocompatibility of novel nanocomposites based on polyhexamethylene carbonate fumarate and nanohydroxyapatite (pp. 605-611)
  257. Sherwood et al. (2002) A three-dimensional osteochondral composite scaffold for articular cartilage repair (pp. 4739-4751)
  258. Shokrollahi et al. (2010) Biological and mechanical properties of novel composites based on supramolecular polycaprolactone and functionalised hydroxyapatite (pp. 209-221)
  259. Shor et al. (2007) Fabrication of three-dimensional polycaprolactone/hydroxyapatite tissue scaffolds and osteoblast-scaffold interactions in vitro (pp. 5291-5297)
  260. Shor et al. (2009) Precision extruding deposition (PED) fabrication of polycaprolactone (PCL) scaffolds for bone tissue engineering
  261. Shuai et al. (2013) In vitro bioactivity and degradability of β-tricalcium phosphate porous scaffold fabricated via selective laser sintering (pp. 1-8)
  262. Simon et al. (2003) Engineered cellular response to scaffold architecture in a rabbit trephine defect (pp. 275-282)
  263. Smay et al. (2002) Colloidal inks for directed assembly of 3-D periodic structures (pp. 5429-5437)
  264. Sobral et al. (2011) Three-dimentional plotted scaffolds with controlled pore size gradients: effect of scaffold geometry on mechnical performance and cell seeding efficiency (pp. 1009-1018)
  265. Stamboulis et al. (2002) Novel biodegradable polymer/bioactive glass composites for tissue engineering applications (pp. 105-109)
  266. Sudarmadji et al. (2011) Investigation of the mechanical properties and porosity relationships in selective laser-sintered polyhedral for functionally graded scaffolds (pp. 530-537)
  267. Sun et al. (2007) The effect of the ionic products of Bioglass® dissolution on human osteoblasts growth cycle in vitro (pp. 281-286)
  268. Suuronen et al. (1998) A 5-year in vitro and in vivo study of the biodegradation of polylactide plates (pp. 604-614)
  269. Tam et al. (1996) Poly(L-lactide) bone plates and screws for internal fixation of mandibular swing osteotomies (pp. 20-24)
  270. Tatakis and Trombelli (1999) Adverse effects associated with a bioabsorbable guided tissue regeneration device in the treatment of human gingival recession defects: a clinicopathologic case report (pp. 542-547)
  271. Tellis et al. (2008) Trabecular scaffolds created using micro CT guided fused deposition modeling (pp. 171-178)
  272. Tesavibul et al. (2012) Processing of 45S5 Bioglass® by lithography-based additive manufacturing (pp. 81-84)
  273. Thein-Han and Misra (2009) Biomimetic chitosan–nanohydroxyapatite composite scaffolds for bone tissue engineering (pp. 1182-1197)
  274. Tian et al. (2012) Biodegradable synthetic polymers: preparation, functionalization and biomedical application (pp. 237-280)
  275. Tirella et al. (2011) PAM2 (Piston Assisted Microsyringe): a new rapid prototyping technique for biofabrication of cell incorporated scaffolds (pp. 229-237)
  276. Tulliani et al. (2013) Development and mechanical characterization of novel ceramic foams fabricated by gel-casting (pp. 1567-1576)
  277. Verma et al. (2002) A possible role of poly-3-hydroxybutyric acid in antibiotic production in streptomyces (pp. 66-69)
  278. Verrier et al. (2004) PDLLA/Bioglass® composites for soft-tissue and hard-tissue engineering: an in vitro cell biology assessment (pp. 3013-3021)
  279. Vozzi and Ahluwalia (2007) Microfabrication for tissue engineering: rethinking the cells-on-a scaffold approach (pp. 1248-1254)
  280. Vozzi et al. (2002) Microsyringe-based deposition of two-dimensional and three-dimensional polymer scaffolds with a well-defined geometry for application to tissue engineering (pp. 1089-1098)
  281. Vozzi et al. (2003) Fabrication of PLGA scaffolds using soft lithography and microsyringe deposition (pp. 2533-2540)
  282. Vozzi et al. (2013) Pressure-activated microsyringe composite scaffold of poly(l-lactic acid) and carbon nanotubes for bone tissue engineering (pp. 528-536)
  283. Wang et al. (2002) A tough biodegradable elastomer (pp. 602-606)
  284. Wang et al. (2004) Precision extruding deposition and characterization of cellular poly-ε-caprolactone tissue scaffolds (pp. 42-49)
  285. Warnke et al. (2010) Ceramic scaffolds produced by computer-assisted 3D printing and sintering: characterization and biocompatibility investigations (pp. 212-217)
  286. Weiss et al. (2009) Two-photon polymerization for microfabrication of three-dimensional scaffolds for tissue engineering application (pp. 384-390)
  287. Weiss et al. (2011) Two-photon polymerization of biocompatible photopolymers for microstructured 3D biointerfaces (pp. B264-B273)
  288. Williams et al. (2005) Bone tissue engineering using polycaprolactone scaffolds fabricated via selective laser sintering (pp. 4817-4827)
  289. Winkel et al. (2012) Sintering of 3D-printed glass/HAp composites (pp. 3387-3393)
  290. Wiria et al. (2007) Poly-ε-caprolactone/hydroxyapatite for tissue engineering scaffold fabrication via selective laser sintering (pp. 1-12)
  291. Wojtowicz et al. (2010) Coating of biomaterial scaffolds with the collagen-mimetic peptide GFOGER for bone defect repair (pp. 2574-2582)
  292. Woodruff and Hutmacher (2010) The return of a forgotten polymer-polycaprolactone in the 21st century (pp. 1217-1256)
  293. Wu et al. (2011) Melt-derived bioactive glass scaffolds produced by a gel-cast foaming technique (pp. 1807-1816)
  294. Xiong et al. (2002) Fabrication of porous scaffolds for bone tissue engineering via low-temperature deposition (pp. 771-776)
  295. Xynos et al. (2001) Gene expression profiling of human osteoblasts following treatment with the ionic products of Bioglass® 45S5 dissolution (pp. 151-157)
  296. Yan et al. (2003) Layered manufacturing of tissue engineering scaffolds via multi-nozzle deposition (pp. 2623-2628)
  297. Yen et al. (2009) Evaluation of chondrocyte growth in the highly porous scaffolds made by fused deposition manufacturing (FDM) filled with type II collagen (pp. 615-624)
  298. Yeong et al. (2004) Rapid prototyping in tissue engineering: challenges and potential (pp. 643-652)
  299. Yeong et al. (2010) Porous polycaprolactone scaffold for cardiac tissue engineering fabricated by selective laser sintering (pp. 2028-2034)
  300. Yin et al. (2003) Preparation and characterization of macroporous chitosan–gelatin/β-tricalcium phosphate composite scaffolds for bone tissue engineering (pp. 844-855)
  301. Zein et al. (2002) Fused deposition modeling of novel scaffold architectures for tissue engineering applications (pp. 1169-1185)
  302. Zhang et al. (2004) Processing and properties of porous poly(L-lactide)/bioactive glass composites (pp. 2489-2500)
  303. Zhang et al. (2008) In vitro biocompatibility of hydroxyapatite-reinforced polymeric composites manufactured by selective laser sintering (pp. 1018-1027)
  304. Zhou et al. (2007) In vitro bone engineering based on polycaprolactone and polycaprolactone–tricalcium phosphate composites (pp. 333-342)
  305. Zhou et al. (2007) Combined marrow stromal cell-sheet techniques and high-strength biodegradable composite scaffolds for engineered functional bone grafts (pp. 814-824)