10.1007/s40204-022-00216-4

Acceleration in healing of infected full-thickness wound with novel antibacterial γ-AlOOH-based nanocomposites

  • Hilda Parastar1,
  • Mohammad Reza Farahpour*2,
  • Rasoul Shokri1,
  • Saeed Jafarirad3,
  • Mohsen Kalantari1,
  1. Department of Microbiology, Biology Research Center, Zanjan Branch, Islamic Azad University, Zanjan, IR
  2. Department of Clinical Sciences, Faculty of Veterinary Medicine, Urmia Branch, Islamic Azad University, Urmia, IR
  3. Department of Organic and Biochemistry, Faculty of Chemistry, University of Tabriz, Tabriz, IR Research Center of Bioscience and Biotechnology, University of Tabriz, Tabriz, IR

Published in Issue 2023-01-04

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

How to Cite

Parastar, H., Farahpour, M. R., Shokri, R., Jafarirad, S., & Kalantari, M. (2023). Acceleration in healing of infected full-thickness wound with novel antibacterial γ-AlOOH-based nanocomposites. Progress in Biomaterials, 12(2 (June 2023). https://doi.org/10.1007/s40204-022-00216-4
Crossref
Scopus
Google Scholar
Europe PMC

Abstract

Abstract This study was conducted to synthesize γ-AlOOH (bohemite)-based nanocomposites (NCs) of Au/γ-AlOOH-NC and its functionalized derivative using chitosan (Au/γ-AlOOH/Ctn-NC) and with the help of one-step Mentha piperita . The physicochemical characteristics of the NCs were investigated. In addition, biomedical properties, such as antibacterial activity under in vitro and in vivo conditions, and cell viability were assessed. Wound healing activity on infected wounds and histological parameters were assessed. The gene expressions of TNF-α, Capase 3, Bcl-2, Cyclin-D1 and FGF-2 were investigated. The TEM and FESEM images showed the sheet-like structure for bohemite in Au/γ-AlOOH-NC with Au nanoparticles in a range of 14–15 nm. The elemental analysis revealed the presence of carbon, oxygen, aluminum, and Au elements in the as-synthesized Au/γ-AlOOH. The results for toxicity showed that the produced nanocomposites did not show any cytotoxicity. Biomedical studies confirmed that Au/γ-AlOOH-NC and Au/γ-AlOOH/Ctn-NC have anti-bacterial properties and could expedite the wound healing process in infected wounds by an increase in collagen biosynthesis. The administration of ointment containing Au/γ-AlOOH-NC and Au/γ-AlOOH/Ctn-NC decreased the expressions of TNF-α, and increased the expressions of Capase 3, Bcl-2, Cyclin-D1 and FGF-2. The novelty of this study was that bohemite and Au nanoparticles can be used as a dressing to accelerate the wound healing process. In green synthesis of Au/γ-AlOOH-NC, phytochemical compounds of the plant extract are appropriate reagents for stabilization and the production of Au/γ-AlOOH-NC. Therefore, the new bohemite-based NCs can be considered as candidate for treatment of infected wounds after future clinical studies.

Keywords

  • Infected wounds,
  • Au/γ-AlOOH nanocomposites,
  • Phytochemical metabolites,
  • Antibacterial properties,
  • Chitosan

References

  1. Abd El-Hack et al. (2020) Antimicrobial and antioxidant properties of chitosan and its derivatives and their applications: a review (pp. 2726-2744) https://doi.org/10.1016/j.ijbiomac.2020.08.153
  2. Aleksandr et al. (2018) Synthesis of antimicrobial AlOOH–Ag composite nanostructures by water oxidation of bimetallic Al–Ag nanoparticles (pp. 36239-36244) https://doi.org/10.1039/C8RA04173C
  3. Al-Musawi et al. (2020) Antibacterial activity of honey/chitosan nanofibers loaded with capsaicin and gold nanoparticles for wound dressing https://doi.org/10.3390/molecules25204770
  4. Andretto et al. (2021) Nanocomposite systems for precise oral delivery of drugs and biologics 11(2) (pp. 445-470) https://doi.org/10.1007/s13346-021-00905-w
  5. Bak et al. (2020) Boehmite enhances hair follicle growth via stimulation of dermal papilla cells by upregulating β-catenin signalling (pp. 341-348) https://doi.org/10.1111/exd.14051
  6. Behzad et al. (2020) A systematic investigation on spectroscopic, conformational, and interactional properties of polypeptide/nanomaterial complex: effects of bio-based synthesized maghemite nanocomposites on human serum albumin (pp. 471-486) https://doi.org/10.1080/1539445X.2020.1716800
  7. Buldain et al. (2021) Modeling the growth and death of Staphylococcus aureus against Melaleuca armillaris essential oil at different pH conditions https://doi.org/10.3390/antibiotics10020222
  8. Daghian et al. (2021) Biological fabrication and electrostatic attractions of new layered silver/talc nanocomposite using Lawsonia inermis L. and its chitosan-capped inorganic/organic hybrid: investigation on acceleration of Staphylococcus aureus and Pseudomonas aeruginosa infected wound healing https://doi.org/10.1016/j.msec.2021.112294
  9. Das and Horton (2016) Antibiotics: achieving the balance between access and excess (pp. 102-104) https://doi.org/10.1016/S0140-6736(15)00729-1
  10. de Araújo et al. (2019) Fibroblast growth factors: a controlling mechanism of skin aging (pp. 275-282) https://doi.org/10.1159/000501145
  11. Desjardins-Park et al. (2018) Fibroblasts and wound healing: an update (pp. 491-495) https://doi.org/10.2217/rme-2018-0073
  12. Farahpour et al. (2017) Hydroethanolic Allium sativum extract accelerates excision wound healing: evidence for roles of mast-cell infiltration and intracytoplasmic carbohydrate ratio (pp. 53-62) https://doi.org/10.1590/s2175-97902017000115079
  13. Forest et al. (2014) Toxicity of boehmite nanoparticles: impact of the ultrafine fraction and of the agglomerates size on cytotoxicity and pro-inflammatory response 26(9) (pp. 545-553) https://doi.org/10.3109/08958378.2014.925993
  14. Gharehpapagh et al. (2021) The biological synthesis of gold/perlite nanocomposite using Urtica dioica extract and its chitosan-capped derivative for healing wounds infected with methicillin-resistant Staphylococcus aureus (pp. 447-456) https://doi.org/10.1016/j.ijbiomac.2021.04.150
  15. Hernández Martínez SP, Rivera González TI, Franco Molina MA, Bollain y Goytia JJ, Martínez Sanmiguel JJ, Zárate Triviño DG, Rodríguez Padilla C (2019) A novel gold calreticulin nanocomposite based on chitosan for wound healing in a diabetic mice model. Nanomaterials 8:75.
  16. https://doi.org/10.3390/nano9010075
  17. Islam et al. (2021) Gelatin-based instant gel-forming volatile spray for wound-dressing application 10(3) (pp. 235-243) https://doi.org/10.1007/s40204-021-00166-3
  18. Jafarirad et al. (2020) Biosynthesis, characterization and structural properties of a novel kind of Ag/ZnO nanocomposites in order to increase its biocompatibility across human A549 cell line (pp. 42-53) https://doi.org/10.1007/s12668-019-00685-1
  19. Jamshed and Jabeen (2022) Pharmacological evaluation of Mentha piperita against urolithiasis: an in vitro and in vivo study 20(1) (pp. 1-15) https://doi.org/10.1177/15593258211073087
  20. Joshi et al. (2020) Interactions of gold and silver nanoparticles with bacterial biofilms: molecular interactions behind inhibition and resistance https://doi.org/10.3390/ijms21207658
  21. Kaiser et al. (2021) Therapy of infected wounds: overcoming clinical challenges by advanced drug delivery systems (pp. 1545-1567) https://doi.org/10.1007/s13346-021-00932-7
  22. Lee et al. (2019) Bioaccumulation of polystyrene nanoplastics and their effect on the toxicity of Au ions in zebrafish embryos 11(7) (pp. 3173-3185) https://doi.org/10.1039/C8NR09321K
  23. Li et al. (2017) Silver inlaid with gold nanoparticle/chitosan wound dressing enhances antibacterial activity and porosity, and promotes wound healing (pp. 3766-3775) https://doi.org/10.1021/acs.biomac.7b01180
  24. Makabenta et al. (2021) Nanomaterial-based therapeutics for antibiotic-resistant bacterial infections (pp. 23-36) https://doi.org/10.1038/s41579-020-0420-1
  25. Mir et al. (2018) Synthetic polymeric biomaterials for wound healing: a review 7(1) (pp. 1-21) https://doi.org/10.1007/s40204-018-0083-4
  26. Modarresi et al. (2019) Topical application of Mentha piperita essential oil accelerates wound healing in infected mice model (pp. 531-537) https://doi.org/10.1007/s10787-018-0510-0
  27. Moetamedipoor et al. (2021) Essential oil chemical diversity of Iranian mints https://doi.org/10.1016/j.indcrop.2021.114039
  28. Mohan et al. (2020) Recent insights into the extraction, characterization, and bioactivities of chitin and chitosan from insects (pp. 17-42) https://doi.org/10.1016/j.tifs.2020.08.016
  29. Mossahebi-Mohammadi et al. (2020) FGF signaling pathway: a key regulator of stem cell pluripotency https://doi.org/10.3389/fcell.2020.00079
  30. Nagai et al. (2012) Fabrication of boehmite and Al2O3 nonwovens from boehmite nanofibres and their potential as the sorbent (pp. 21225-21231) https://doi.org/10.1039/C2JM33914E
  31. Naraginti et al. (2016) Amelioration of excision wounds by topical application of green synthesized, formulated silver and gold nanoparticles in albino Wistar rats (pp. 293-300) https://doi.org/10.1016/j.msec.2016.01.069
  32. Negut et al. (2018) Treatment strategies for infected wounds 23(9) https://doi.org/10.3390/molecules23092392
  33. Paglia et al. (2006) Fine-scale nanostructure in γ-Al2O3 (pp. 3242-3248) https://doi.org/10.1021/cm060277j
  34. Pajerski et al. (2019) Attachment efficiency of gold nanoparticles by Gram-positive and Gram-negative bacterial strains governed by surface charges (pp. 1-2) https://doi.org/10.1007/s11051-019-4617-z
  35. Parvekar et al. (2020) The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of silver nanoparticles against Staphylococcus aureus (pp. 105-109) https://doi.org/10.1080/26415275.2020.1796674
  36. Popgeorgiev et al. (2018) Subcellular localization and dynamics of the Bcl-2 family of proteins https://doi.org/10.3389/fcell.2018.00013
  37. Pourkarim et al. (2022) Comparison effects of platelet-rich plasma on healing of infected and non-infected excision wounds by the modulation of the expression of inflammatory mediators: experimental research (pp. 1-9) https://doi.org/10.1007/s00068-022-01907-0
  38. Rashed (2017) Antibacterial activity of boehmite nanoparticles synthesized by arc discharge in deionized water technique (pp. 58-65) https://doi.org/10.22401/JNUS.20.1.08
  39. Raziyeva et al. (2021) Immunology of acute and chronic wound healing https://doi.org/10.3390/biom11050700
  40. Rezaei et al. (2019) Modulation of secondary metabolite profiles by biologically synthesized MgO/perlite nanocomposites in Melissa officinalis plant organ cultures https://doi.org/10.1016/j.jhazmat.2019.120878
  41. Ritsu et al. (2017) Critical role of tumor necrosis factor-α in the early process of wound healing in skin (pp. 14-19) https://doi.org/10.1016/j.jdds.2016.09.001
  42. Sathiyaraj et al. (2021) Biosynthesis, characterization, and antibacterial activity of gold nanoparticles (pp. 1842-1847) https://doi.org/10.1016/j.jiph.2021.10.007
  43. Shen et al. (2017) Polyethylenimine-based micro/nanoparticles as vaccine adjuvants (pp. 5443-5460) https://doi.org/10.2147/IJN.S137980
  44. Shkir et al. (2018) A facile synthesis of Au-nanoparticles decorated PbI2 single crystalline nanosheets for optoelectronic device applications (pp. 1-10) https://doi.org/10.1038/s41598-018-32038-5
  45. Silva (2020) A descriptive overview of the medical uses given to Mentha aromatic herbs throughout history https://doi.org/10.3390/biology9120484
  46. Slavin et al. (2017) Metal nanoparticles: understanding the mechanisms behind antibacterial activity (pp. 1-20) https://doi.org/10.1186/s12951-017-0308-z
  47. Solovev et al. (2019) Sol-gel derived boehmite nanostructures is a versatile nanoplatform for biomedical applications (pp. 1-4) https://doi.org/10.1038/s41598-018-37589-1
  48. Thakur et al. (2019) Nano-engineered lipid-polymer hybrid nanoparticles of fusidic acid: an investigative study on dermatokinetics profile and MRSA-infected burn wound model (pp. 748-763) https://doi.org/10.1007/s13346-019-00616-3
  49. Wang et al. (2020) Preparation, characterization and evaluation of a new film based on chitosan, arginine and gold nanoparticle derivatives for wound-healing efficacy (pp. 20886-20899) https://doi.org/10.1039/D0RA03704D
  50. Wilkinson and Hardman (2020) Wound healing: cellular mechanisms and pathological outcomes (pp. 1748-1763) https://doi.org/10.1098/rsob.200223
  51. Yazdanian et al. (2022) The potential application of green-synthesized metal nanoparticles in dentistry: a comprehensive review https://doi.org/10.1155/2022/2311910
  52. Yosefzon et al. (2018) Caspase-3 regulates YAP-dependent cell proliferation and organ size (pp. 573-587) https://doi.org/10.1016/j.molcel.2018.04.019
  53. Zhou et al. (2016) Quercetin reduces cyclin D1 activity and induces G1 phase arrest in HepG2 cells (pp. 516-522) https://doi.org/10.3892/ol.2016.4639