10.1007/s40204-022-00180-z

A hyaluronic acid/PVA electrospun coating on 3D printed PLA scaffold for orthopedic application

Show Details XML
  • Mina Farsi1,
  • Azadeh Asefnejad*1,
  • Hadi Baharifar2,
  1. Department of Biomedical Engineering, Science and Research Branch, Islamic Azad University, Tehran, IR
  2. Department of Medical Nanotechnology, Applied Biophotonics Research Center, Science and Research Branch, Islamic Azad University, Tehran, 1477893855, IR

Published in Issue 2022-01-22

How to Cite

Farsi, M., Asefnejad, A., & Baharifar, H. (2022). A hyaluronic acid/PVA electrospun coating on 3D printed PLA scaffold for orthopedic application. Progress in Biomaterials, 11(1 (March 2022). https://doi.org/10.1007/s40204-022-00180-z
Crossref
Scopus
Google Scholar
Europe PMC

Abstract

Abstract The need for bone tissue replacement, repair and regeneration for orthopedic application is constantly growing. Therefore, the application of cartilage substitute due to the lack of donors as well as biocompatibility leads to immune system rejection. In order to overcome these drawbacks, researchers have used porous scaffold as an option for bone transplantation. In this study, poly-lactic acid (PLA) scaffolds were prepared for cartilage application by fused deposition modeling (FDM) technique and then coated by electrospinning with polyvinyl alcohol (PVA) and hyaluronic acid (HLA) fibers. Hybrid electrospinning (ELS) method was used to produce porous scaffolds from HLA–PVA polymers. The printed scaffold was coated using FDM technique and the mechanical and biological investigation was performed on the polymeric composite specimen. The functional group and morphological behavior were investigated using Fourier-transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM) techniques. The obtained porous scaffold has hydrophilic properties as the PVA and HLA were coated on the PLA. The porous 3D-printed scaffold containing PLA/PVA/HLA scaffold does not show any toxicity in MTT evaluation after 1, 3 and 7 days. The SEM image confirmed the cell adhesion of the chondrite to the scaffold. Also, the mechanical performances of the sample, such as elastic modulus and compressive strength, were evaluated by compression test. By electro-spun coating, the elastic module of PVA/PLA and PLA/PVA/HLA scaffolds has increased to 18.31 ± 0.29 MPa and 19.25 ± 0.38 MPa. Also, the tensile strength of these two porous scaffolds has reached 6.11 ± 0.42 MPa and 6.56 ± 0.14 MPa, respectively. The failure strain of 3D printed PLA scaffold was reported to be 53 ± 0.21% and this value was reduced to 47 ± 0.62% and 42 ± 0.22% in PVA/PLA and PLA/PVA/HLA scaffolds. The cells’ growth on the porous scaffolds showed a broad, spindle-shaped and regular shape. The obtained results of the chemical, physical and biological analyses showed that porous PLA/PVA/HLA scaffold has potential applications in cartilage construction.

Keywords

  • Poly-lactic acid,
  • Polyvinyl alcohol,
  • Hyaluronic acid,
  • 3D printing,
  • Tissue engineering

References

  1. Abasalta et al. (2021) Fabrication of carboxymethyl chitosan/poly (ε-caprolactone)/doxorubicin/nickel ferrite core-shell fibers for controlled release of doxorubicin against breast cancer
  2. Abid et al. (2021) Atherogenic indices in clinical practice and biomedical research: a short review: atherogenic indices and cardiovascular diseases 2(02) (pp. 59-69)
  3. Aghdam et al. (2020) Effect of calcium silicate nanoparticle on surface feature of calcium phosphates hybrid bio-nanocomposite using for bone substitute application (pp. 917-929)
  4. Alwan et al. (2021) Safety assessment and biochemical evaluation of biogenic silver nanoparticles (using bark extract of C.zeylanicum) in Rattusnorvegicus rats: safety of biofabricated AgNPs (using Cinnamomum zeylanicum extract) 2(03) (pp. 138-150)
  5. Ansari (2015) Investigations of polyethylene glycol mediated ternary molecular inclusion complexes of silymarin with beta cyclodextrins (pp. 26-31)
  6. Ansari and Alshahrani (2019) Nano-encapsulation and characterization of baricitinib using poly-lactic-glycolic acid co-polymer 27(4) (pp. 491-501)
  7. Awad et al. (2004) Chondrogenic differentiation of adipose-derived adult stem cells in agarose, alginate, and gelatin scaffolds 25(16) (pp. 3211-3222)
  8. Bagher et al. (2020) Wound healing with alginate/chitosan hydrogel containing hesperidin in rat model
  9. Bagherifard et al. (2020) Improvement in osseointegration of tricalcium phosphate-zircon for orthopedic applications: an in vitro and in vivo evaluation (pp. 1681-1693)
  10. Bomhard et al. (2013) Prefabrication of 3D cartilage contructs: towards a tissue engineered auricle–a model tested in rabbits 8(8)
  11. Cheng et al. (2021) A multilayer biomimetic chitosan-gelatin-fluorohydroxyapatite cartilage scaffold using for regenerative medicine application (pp. 1761-1777)
  12. Chung and Burdick (2008) Engineering cartilage tissue 60(2) (pp. 243-262)
  13. Danaboina and Neerati (2020) Evidence-based P-glycoprotein inhibition by green tea extract enhanced the oral bioavailability of atorvastatin: from animal and human experimental studies 11(2)
  14. Dong et al. (2021) Investigation of the mechanical properties of a shapeless electroconductive scaffold: addition of akermanite nanoparticles and using a freeze-drying technique
  15. Du et al. (2021) A femoral shape porous scaffold bio-nanocomposite fabricated using 3D printing and freeze-drying technique for orthopedic application
  16. Esmaeili et al. (2019) An artificial blood vessel fabricated by 3D printing for pharmaceutical application 6(3) (pp. 183-194)
  17. Foroutan et al. (2021) A porous sodium alginate-CaSiO3 polymer reinforced with graphene nanosheet: fabrication and optimality analysis 22(2) (pp. 540-549)
  18. Garcia-Fuentes et al. (2009) Silk fibroin/hyaluronan scaffolds for human mesenchymal stem cell culture in tissue engineering 30(28) (pp. 5068-5076)
  19. Ghadirinejad et al. (2021) A fuzzy logic model and a neuro-fuzzy system development on supercritical CO2 regeneration of Ni/Al2O3 catalysts
  20. Ghahramani and Javanmardi (2021) Graphene oxide quantum dots and their applications via stem cells: a mini-review 2(3) (pp. 54-56)
  21. Ghahramani et al. (2021) Graphene Quantum Dots And Their Applications: a mini-review (pp. 53-59)
  22. Halim and Ismail (2018) Potential therapeutic effects of olea Europaea (olive) fruit oil as neuroprotective agent against neurotoxicity induced opioid 3(4) (pp. 159-159)
  23. Halim et al. (2020) Novel potential Centellaasiatica extract in ameliorating neurotoxicity induced oxidative stress in chronic morphine dependant rat model 23(3and4) (pp. 79-83)
  24. Hein et al. (2020) Associations AMONG ACEs, health behavior, and veteran health by service era 44(6) (pp. 876-892)
  25. Iranmanesh et al. (2021) Application of 3D bioprinters for dental pulp regeneration and tissue engineering (porous architecture) https://doi.org/10.1007/s11242-021-01618-x
  26. Jamnezhad et al. (2020) Development and investigation of novel alginate-hyaluronic acid bone fillers using freeze drying technique for orthopedic field 5(4) (pp. 306-315)
  27. Jiang et al. (2020) Clinical application status of articular cartilage regeneration techniques: tissue-engineered cartilage brings new hope https://doi.org/10.1155/2020/5690252
  28. Kaur et al. (2018) Synthesis of cinnamic acid amide derivatives and the biological evaluation of A-glucosidase inhibitory activity 21(3) (pp. 216-220)
  29. Khandan et al. (2020) Fabrication and characterization of porous bioceramic-magnetite biocomposite for maxillofacial fractures application 11(3)
  30. Kianfar (2019) Nanozeolites: synthesized, properties, applications 91(2) (pp. 415-429)
  31. Kim et al. (2008) Hybrid process for fabricating 3D hierarchical scaffolds combining rapid prototyping and electrospinning 29(19) (pp. 1577-1581)
  32. Li et al. (2021) Experimental measurement and simulation of mechanical strength and biological behavior of porous bony scaffold coated with alginate-hydroxyapatite for femoral applications
  33. Margiana et al. (2019) Review on cyclic adenosine monophosphate signaling pathway (cAMP), DLK signaling pathway, RAS/RAF signaling, retinoic acid signaling, phosphatidylinositol 3-kinase (PI3K) as the signaling pathways involved in peripheral neuronal generation 11(1) (pp. 181-198)
  34. Murphy et al. (2020) Articular cartilage regeneration by activated skeletal stem cells 26(10) (pp. 1583-1592)
  35. Nabishah et al. (1990) Effects of steroid hormones pretreatment on isoprenaline-induced cyclic adenosine 3', 5'-monophosphate in rat lung 21(6) (pp. 935-938)
  36. Okamoto and John (2013) Synthetic biopolymer nanocomposites for tissue engineering scaffolds 38(10–11) (pp. 1487-1503)
  37. Othman et al. (2019) The anti-angiogenic properties of Morindacitrifolia L. (Mengkudu) leaves using chicken chorioallantoic membrane (CAM) assay 11(1) (pp. 12-15)
  38. Pandey et al. (2020) Kirsten rat sarcoma virus protein overexpression in adenocarcinoma lung: Association with clinicopathological and histomorphological features
  39. Park et al. (2004) Characteristics of tissue-engineered cartilage from human auricular chondrocytes 25(12) (pp. 2363-2369)
  40. Park et al. (2019) An injectable, click-crosslinked, cytomodulin-modified hyaluronic acid hydrogel for cartilage tissue engineering 11(1) (pp. 1-16)
  41. Raisi et al. (2020) Preparation, characterization, and antibacterial studies of N, O-carboxymethyl chitosan as a wound dressing for bedsore application 9(4) (pp. 181-188)
  42. Raisi et al. (2022) A soft tissue fabricated using a freeze-drying technique with carboxymethyl chitosan and nanoparticles for promoting effects on wound healing 7(4) (pp. 262-274)
  43. Razeghian et al. (2021) Mesenchymal stem/stromal cells as a vehicle for cytokine delivery: an emerging approach for tumor immunotherapy https://doi.org/10.3389/fmed.2021.721174
  44. Sahmani et al. (2019) Nonlinear resonance investigation of nanoclay based bio-nanocomposite scaffolds with enhanced properties for bone substitute applications (pp. 636-653)
  45. Sakaguchi et al. (2005) Comparison of human stem cells derived from various mesenchymal tissues: superiority of synovium as a cell source 52(8) (pp. 2521-2529)
  46. Salmani et al. (2020) Therapeutic effect of magnetic nanoparticles on calcium silicate bioceramic in alternating field for biomedical application 46(17) (pp. 27299-27307)
  47. Salmani et al. (2020) Synergic effects of magnetic nanoparticles on hyperthermia-based therapy and controlled drug delivery for bone substitute application (pp. 2809-2820)
  48. Shirani et al. (2020) A narrative review of COVID-19: the new pandemic disease 45(4)
  49. Sowmya et al. (2020) Development of clinico-histopathological predictive model for the assessment of metastatic risk of oral squamous cell carcinoma
  50. Sun et al. (2021) Self-healing polymers using electrosprayed microcapsules containing oil: molecular dynamics simulation and experimental studies
  51. Tolou et al. (2013) The effect of adding Tio2 nanoparticles on dental amalgam properties 10(2)
  52. Wardani et al. (2019) Development of nonalcoholic fatty liver disease model by high-fat diet in rats https://doi.org/10.1515/jbcpp-2019-0258
  53. Yang et al. (2018) Neuro-protection of Abelmoschusesculentus L. against diabetic neuropathy 11(3)
  54. You SH, Yoon MY, Moon JS (2021) Antioxidant and anti-inflammatory activity study of fulvic acid. J Nat Sci Biol Med 12(3)
  55. Zeylabi et al. (2010) Endodontic management of a fused mandibular third molar and distomolar: a case report 36(1) (pp. 29-31)