10.1007/s40204-021-00178-z

Fe3O4 nanoparticles coated with carboxymethyl chitosan containing curcumin in combination with hyperthermia induced apoptosis in breast cancer cells

  • Negin Pazouki1,
  • Shiva Irani*1,
  • Nafiseh Olov2,
  • Seyed Mohammad Atyabi3,
  • Shadab Bagheri-Khoulenjani2,
  1. Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, IR
  2. Department of Polymer and Color Engineering, Amirkabir University of Technology, Tehran, IR
  3. Department of Nanobiotechnology, Pasteur Institute of Iran, Tehran, IR

Published in Issue 2022-01-13

How to Cite

Pazouki, N., Irani, S., Olov, N., Atyabi, S. M., & Bagheri-Khoulenjani, S. (2022). Fe3O4 nanoparticles coated with carboxymethyl chitosan containing curcumin in combination with hyperthermia induced apoptosis in breast cancer cells. Progress in Biomaterials, 11(1 (March 2022). https://doi.org/10.1007/s40204-021-00178-z
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Abstract

Abstract Many studies have demonstrated that curcumin has potential anticancer properties. This research aims to study the effect of iron (II, III) oxide (Fe 3 O 4 ) nanoparticles coated with carboxymethyl chitosan containing curcumin combination with hyperthermia on breast cancer cells. Magnetic nanoparticles coated with carboxymethyl chitosan containing curcumin (MNP-CMC-CUR) were prepared and specified. MCF-7, MDA-MB-231, and human fibroblast cells were treated with free curcumin and MNP-CMC-CUR at concentrations of 0–60 µM and at different time points. A combined therapy of MNP-CMC-CUR and hyperthermia was performed on MCF-7 cells. The cytotoxicity of curcumin and MNP-CMC-CUR combined with hyperthermia was assessed by MTT. The changes in TP53 and CASPASE3 gene expression were evaluated using real-time PCR. Both cell apoptosis and cell cycle were studied by Annexin/PI staining. The results of MTT showed that the IC 50 amount of MNP-CMC-CUR has significantly decreased compared to free curcumin ( p  < 0.05) and MNP-CMC-CUR in combination with the hyperthermia, and significantly reducing the metabolic activity of the cells ( p  < 0.05). Real-time PCR results revealed the up-regulation of TP53 and CASPASE3 ( p  < 0.05). The combinational therapy-induced cell apoptosis (64.51%) and sub-G1 cell cycle were arrested in MCF-7 cells. Based on these observations, a combination of MNP-CMC-CUR with hyperthermia could inhibit the proliferation of MCF-7 cells.

Keywords

  • Curcumin,
  • Breast cancer,
  • Carboxymethyl chitosan,
  • Magnetic nanoparticle,
  • Hyperthermia

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