10.1007/s40204-021-00176-1

Multi-antibacterial agent-based electrospun polycaprolactone for active wound dressing

XML
  • Fatemeh Safdari1,
  • Maryam Darya Gholipour1,
  • Azam Ghadami1,
  • Mahdi Saeed*2,
  • Mojgan Zandi3,
  1. Department of Chemical and Polymer Engineering, Central Tehran Branch, Islamic Azad University, Tehran, IR
  2. Soft Tissue Engineering Research Center, Tissue Engineering and Regenerative Medicine Institute, Central Tehran Branch, Islamic Azad University, Tehran, IR Department of Biomedical Engineering, Central Tehran Branch, Islamic Azad University, Tehran, IR
  3. Department of Biomaterials, Iran Polymer and Petrochemical Institute, Tehran, IR

Published in Issue 2022-01-29

How to Cite

Safdari, F., Gholipour, M. D., Ghadami, A., Saeed, M., & Zandi, M. (2022). Multi-antibacterial agent-based electrospun polycaprolactone for active wound dressing. Progress in Biomaterials, 11(1 (March 2022). https://doi.org/10.1007/s40204-021-00176-1
Crossref
Scopus
Google Scholar
Europe PMC

Abstract

Abstract Today, due to the greater knowledge of the side effects of chemical drugs and the favorable pharmacological properties of herbal compounds, the use of these compounds is increasing. Since wounds need fast and efficient healing, wound dressing fabrication methods play an important role in wound healing. In this research, electrospinning process was used to prepare samples. Natural antibacterial compounds, such as curcumin, piperine, eugenol, and rutin were loaded in electrospun nano-fibrous based on polycaprolactone. Three-component novel systems of curcumin–piperine–eugenol (PCPiEu), and curcumin–piperine–rutin (PCPiR) were designed and prepared. Their synergistic effect was investigated and also compared with one- and two-component systems. The results showed that medium diameter nanofibers of PCPiEu and PCPiR samples was 198.38 and 142.60, respectively, and they were obtained in smooth, uniform and bead free morphology using optimization of process parameters. The amount of water absorption and water vapor permeability of the three-component samples were in the appropriate range (8.33–10.42 mg cm 2  h −1 ) for wound dressings. The mechanical properties of samples were reduced compared to the control sample, which required further investigation. Antibacterial tests showed good results for partial toxicity of PCPiEu and PCPiR samples. Antibacterial tests showed minor toxicity in PCPiR samples and good results were obtained for PCPiEu samples. In addition, the results showed that PCPiEu and PCPiR samples exhibited antibacterial activity against Gram-positive bacterium Staphylococcus aureus and Gram-negative Enterococcus faecalis bacterium, so that killing ability of 74% and 75% against Gram-positive bacterium and 99.47% and 96.88% against Gram-negative bacterium were obtained for these three systems, respectively.

Keywords

  • Antibacterial wound dressing,
  • Electrospun polycaprolactone,
  • Rutin,
  • Curcumin

References

  1. Abbas et al. (2019) Wound healing potential of curcumin cross-linked chitosan/polyvinyl alcohol (pp. 871-876) https://doi.org/10.1016/j.ijbiomac.2019.08.153
  2. Abdallah et al. (2018) Synergistic antiproliferative effects of curcumin and celecoxib in hepatocellular carcinoma HepG2 cells 391(12) (pp. 1399-1410) https://doi.org/10.1007/s00210-018-1557-6
  3. Aggarwal et al. (2007) Springer Science & Business Media https://doi.org/10.1007/978-0-387-46401-5
  4. Anitha et al. (2011) Preparation, characterization, in vitro drug release and biological studies of curcumin loaded dextran sulphate–chitosan nanoparticles 84(3) (pp. 1158-1164) https://doi.org/10.1016/j.carbpol.2011.01.005
  5. Arct et al. (2002) Common cosmetic hydrophilic ingredients as penetration modifiers of flavonoids 24(6) (pp. 357-366) https://doi.org/10.1046/j.1467-2494.2002.00166.x
  6. Baspinar et al. (2018) Curcumin and piperine loaded zein-chitosan nanoparticles: development and in-vitro characterisation 26(3) (pp. 323-334) https://doi.org/10.1016/j.jsps.2018.01.010
  7. Bolat et al. (2020) Curcumin-and piperine-loaded emulsomes as combinational treatment approach enhance the anticancer activity of curcumin on HCT116 colorectal cancer model https://doi.org/10.3389/fbioe.2020.00050
  8. Bui et al. (2014) Fabrication and characterization of electrospun curcumin-loaded polycaprolactone-polyethylene glycol nanofibers for enhanced wound healing 22(12) (pp. 1288-1296) https://doi.org/10.1007/s13233-014-2179-6
  9. Calaf et al. (2018) Curcumin and paclitaxel induce cell death in breast cancer cell lines 40(4) (pp. 2381-2388) https://doi.org/10.3892/or.2018.6603
  10. Corona-Gomez et al. (2016) Effect of nanoparticle incorporation and surface coating on mechanical properties of bone scaffolds: a brief review 7(3) https://doi.org/10.3390/jfb7030018
  11. Danafar and Sharafi (2018) Co-delivery of sulforaphane and curcumin with pegylated iron oxide-gold core shell nanoparticles for delivery to breast cancer cell line 17(2)
  12. Doello et al. (2018) Latest in vitro and in vivo assay, clinical trials and patents in cancer treatment using curcumin: a literature review 70(4) (pp. 569-578) https://doi.org/10.1080/01635581.2018.1464347
  13. Elsner et al. (2010) Novel biodegradable composite wound dressings with controlled release of antibiotics: microstructure, mechanical and physical properties 93(2) (pp. 425-435) https://doi.org/10.1002/jbm.b.31599
  14. Eskandarinia et al. (2020) A novel bilayer wound dressing composed of a dense polyurethane/propolis membrane and a biodegradable polycaprolactone/gelatin nanofibrous scaffold 10(1) (pp. 1-15) https://doi.org/10.1002/jbm.b.31599
  15. Ganeshpurkar and Saluja (2017) The pharmacological potential of rutin 25(2) (pp. 149-164) https://doi.org/10.1016/j.jsps.2016.04.025
  16. Jain et al. (2016) Engineering a piperine eluting nanofibrous patch for cancer treatment 2(8) (pp. 1376-1385) https://doi.org/10.1021/acsbiomaterials.6b00297
  17. Kanai et al. (2011) A phase I/II study of gemcitabine-based chemotherapy plus curcumin for patients with gemcitabine-resistant pancreatic cancer 68(1) (pp. 157-164) https://doi.org/10.1007/s00280-010-1470-2
  18. Kancheva et al. (2015) Enhancing the mechanical properties of electrospun polyester mats by heat treatment 9(1) (pp. 49-65) https://doi.org/10.3144/expresspolymlett.2015.6
  19. Kunnumakkara et al. (2017) Curcumin, the golden nutraceutical: multitargeting for multiple chronic diseases 174(11) (pp. 1325-1348) https://doi.org/10.1111/bph.13621
  20. Lee et al. (2018) Piperine-mediated drug interactions and formulation strategy for piperine: recent advances and future perspectives 14(1) (pp. 43-57) https://doi.org/10.1080/17425255.2018.1418854
  21. Li et al. (2015) Curcumin-Eudragit® E PO solid dispersion: a simple and potent method to solve the problems of curcumin (pp. 322-332) https://doi.org/10.1016/j.ejpb.2015.06.002
  22. Li et al. (2017) Construction and comparison of different nanocarriers for co-delivery of cisplatin and curcumin: a synergistic combination nanotherapy for cervical cancer (pp. 628-636) https://doi.org/10.1016/j.biopha.2016.12.042
  23. Li et al. (2021) Fabrication of eugenol loaded gelatin nanofibers by electrospinning technique as active packaging material https://doi.org/10.1016/j.lwt.2020.110800
  24. Liakos et al. (2015) Fibrous wound dressings encapsulating essential oils as natural antimicrobial agents 3(8) (pp. 1583-1589) https://doi.org/10.1039/C4TB01974A
  25. Liu et al. (2012) Preparation and characterization of cationic curcumin nanoparticles for improvement of cellular uptake 90(1) (pp. 16-22) https://doi.org/10.1016/j.carbpol.2012.04.036
  26. Madusanka et al. (2015) A curcumin activated carboxymethyl cellulose–montmorillonite clay nanocomposite having enhanced curcumin release in aqueous media (pp. 695-699) https://doi.org/10.1016/j.carbpol.2015.08.030
  27. Malikmammadov et al. (2018) PCL and PCL-based materials in biomedical applications 29(7–9) (pp. 863-893) https://doi.org/10.1080/09205063.2017.1394711
  28. Mele (2016) Electrospinning of natural polymers for advanced wound care: towards responsive and adaptive dressings 4(28) (pp. 4801-4812) https://doi.org/10.1039/C6TB00804F
  29. Merrell et al. (2009) Curcumin loaded poly (ε-caprolactone) nanofibers: diabetic wound dressing with antioxidant and anti-inflammatory properties 36(12) https://doi.org/10.1111/j.1440-1681.2009.05216.x
  30. Mochane et al. (2019) Morphology and properties of electrospun PCL and its composites for medical applications: a mini review 9(11) https://doi.org/10.3390/app9112205
  31. Mouro et al. (2019) Emulsion electrospun fiber mats of PCL/PVA/chitosan and eugenol for wound dressing applications https://doi.org/10.1155/2019/9859506
  32. Mutlu et al. (2018) Curcumin-loaded electrospun PHBV nanofibers as potential wound-dressing material (pp. 185-193) https://doi.org/10.1016/j.jddst.2017.09.017
  33. Naseri-Nosar and Ziora (2018) Wound dressings from naturally-occurring polymers: a review on homopolysaccharide-based composites (pp. 379-398) https://doi.org/10.1016/j.carbpol.2018.02.003
  34. Nelson et al. (2017) The essential medicinal chemistry of curcumin: miniperspective 60(5) (pp. 1620-1637) https://doi.org/10.1021/acs.jmedchem.6b00975
  35. Qiu et al. (2013) Doxorubicin-loaded electrospun poly (l-lactic acid)/mesoporous silica nanoparticles composite nanofibers for potential postsurgical cancer treatment B1(36) (pp. 4601-4611) https://doi.org/10.1039/C3TB20636J
  36. Ranjbar-Mohammadi and Bahrami (2016) Electrospun curcumin loaded poly (ε-caprolactone)/gum tragacanth nanofibers for biomedical application (pp. 448-456) https://doi.org/10.1016/j.ijbiomac.2015.12.024
  37. Sadeghianmaryan et al. (2020) Curcumin-loaded electrospun polycaprolactone/montmorillonite nanocomposite: wound dressing application with anti-bacterial and low cell toxicity properties 31(2) (pp. 169-187) https://doi.org/10.1080/09205063.2019.1680928
  38. Saeed et al. (2017) Designing and fabrication of curcumin loaded PCL/PVA multi-layer nanofibrous eletrospun structures as active wound dressing 6(1) (pp. 39-48) https://doi.org/10.1007/s40204-017-0062-1
  39. Sethiya et al. (2020) Current trends in drug delivery system of curcumin and its therapeutic applications 20(13) (pp. 1190-1232) https://doi.org/10.2174/1389557520666200429103647
  40. Sethuram et al. (2021) Eugenol micro-emulsion reinforced with silver nanocomposite electrospun mats for wound dressing strategies 2(9) (pp. 2971-2988) https://doi.org/10.1039/D1MA00103E
  41. Shababdoust et al. (2018) Fabrication of curcumin-loaded electrospun nanofiberous polyurethanes with anti-bacterial activity 7(1) (pp. 23-33) https://doi.org/10.1007/s40204-017-0079-5
  42. Suarato et al. (2018) Borrowing from nature: biopolymers and biocomposites as smart wound care materials https://doi.org/10.3389/fbioe.2018.00137
  43. Tang et al. (2017) Solid lipid nanoparticles with TPGS and Brij 78: a co-delivery vehicle of curcumin and piperine for reversing P-glycoprotein-mediated multidrug resistance in vitro 13(1) (pp. 389-395) https://doi.org/10.3892/ol.2016.5421
  44. Vig et al. (2017) Advances in skin regeneration using tissue engineering 18(4) https://doi.org/10.3390/ijms18040789
  45. Yan et al. (2016) Targeted nanomedicine for prostate cancer therapy: docetaxel and curcumin co-encapsulated lipid–polymer hybrid nanoparticles for the enhanced anti-tumor activity in vitro and in vivo 23(5) (pp. 1757-1762) https://doi.org/10.3109/10717544.2015.1069423
  46. Yang et al. (2017) pH multistage responsive micellar system with charge-switch and PEG layer detachment for co-delivery of paclitaxel and curcumin to synergistically eliminate breast cancer stem cells (pp. 53-67) https://doi.org/10.1016/j.biomaterials.2017.09.013
  47. Yao et al. (2017) Novel bilayer wound dressing based on electrospun gelatin/keratin nanofibrous mats for skin wound repair (pp. 533-540) https://doi.org/10.1016/j.msec.2017.05.076
  48. Zaidi Z, Lanigan SW (2010) Skin: structure and function. In: Dermatology in clinical practice. Springer, pp 1–15
  49. Zhang et al. (2017) Electrospun nanofibres containing antimicrobial plant extracts 7(2) https://doi.org/10.3390/nano7020042
  50. Zhang et al. (2019) Fabrication and characterization of silk fibroin/curcumin sustained-release film 12(20) https://doi.org/10.3390/ma12203340
  51. Zhou et al. (2021) Electrospun chitosan oligosaccharide/polycaprolactone nanofibers loaded with wound-healing compounds of Rutin and Quercetin as antibacterial dressings (pp. 1145-1154) https://doi.org/10.1016/j.ijbiomac.2021.05.031