10.1007/s40204-021-00174-3

Design expert assisted formulation, characterization and optimization of microemulsion based solid lipid nanoparticles of repaglinide

  • Balaji Maddiboyina*1,
  • Vikas Jhawat2,
  • Ramya Krishna Nakkala1,
  • Prasanna Kumar Desu3,
  • Sivaraman Gandhi4,
  1. Department of Pharmacy, NRK & KSR Gupta College of Pharmacy, Tenali, Andhra Pradesh, 522001, IN
  2. Department of Pharmacy, School of Medical and Allied Sciences, GD Goenka University, Gurgaon, IN
  3. College of Pharmacy, Koneru Lakshmaiah Education Foundation, Vaddeswaram, Andhra Pradesh, IN
  4. Department of Chemistry, Gandhigram Rural Institute Deemed University, Dindigul, Tamil Nadu, IN

Published in Issue 2021-11-23

How to Cite

Maddiboyina, B., Jhawat, V., Nakkala, R. K., Desu, P. K., & Gandhi, S. (2021). Design expert assisted formulation, characterization and optimization of microemulsion based solid lipid nanoparticles of repaglinide. Progress in Biomaterials, 10(4 (December 2021). https://doi.org/10.1007/s40204-021-00174-3
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Abstract

Abstract Repaglinide, a member of the meglitinide class of drugs, is a new anti-diabetic agent that is utilized as an oral hypoglycemic agent. Using glyceryl monostearate, cetyl palmitate, and tristearin as lipids and poloxamer 188 as a surfactant, repaglinide-loaded solid lipid nanoparticles were created. Solid lipid nanoparticles were prepared utilizing an o/w microemulsion technique, which included the lipids glyceryl monostearate and tristearin, as well as waxes such as cetyl palmitate and the surfactant poloxamer 188. The mean particle size of the solid lipid nanoparticles formed was around 339 nm, with an entrapment efficiency of 82.20%. In-vitro release studies continued to be conducted using the dialysis bag diffusion technique. Within 12 h, the cumulative drug release was 88.4%. The results indicate that repaglinide was released more slowly from solid lipid nanoparticles made from tristearin and glyceryl monostearate in an equal ratio. Tristearin found the controlled release and extreme entrapment from other lipid carriers like glyceryl monostearate and cetyl palmitate. Differential scanning calorimetry demonstrates that repaglinide is entangled in amorphous or molecular state within solid lipid nanoparticles. SEM microscopy revealed that the produced repaglinide solid lipid nanoparticles had a spherical shape. After one month of storage at 2–8 °C, short-term stability testing revealed no significant alteration.

Keywords

  • Solid lipid nanoparticles,
  • Diabetes,
  • Repaglinide,
  • Microemulsion technique

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