10.1007/s40204-021-00172-5

Chitosan-coated pore wall polycaprolactone three-dimensional porous scaffolds fabricated by porogen leaching method for bone tissue engineering: a comparative study on blending technique to fabricate scaffolds

  • Deepak Poddar1,
  • Misba Majood2,
  • Ankita Singh1,
  • Sujata Mohanty2,
  • Purnima Jain*1,
  1. Department of Chemistry, Netaji Subhas Institute of Technology, University of Delhi, New Delhi, 110078, IN
  2. Stem Cell Facility, DBT-Centre of Excellence for Stem Cell Research, All India Institute of Medical Sciences, New Delhi, 110029, IN

Published in Issue 2021-11-25

How to Cite

Poddar, D., Majood, M., Singh, A., Mohanty, S., & Jain, P. (2021). Chitosan-coated pore wall polycaprolactone three-dimensional porous scaffolds fabricated by porogen leaching method for bone tissue engineering: a comparative study on blending technique to fabricate scaffolds. Progress in Biomaterials, 10(4 (December 2021). https://doi.org/10.1007/s40204-021-00172-5
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Abstract

Abstract One of the significant challenges in the fabrication of scaffolds for tissue engineering lies in the direct interaction of bioactive agents with cells in the scaffolds matrix, which curbs the effectiveness of bioactive agents resulting in diminished cell recognition and attachment ability of the scaffolds. Here, three-dimensional porous scaffolds were fabricated using polycaprolactone (PCL) and chitosan, by two approaches, i.e., blending and surface coating to compare their overall effectiveness. Blended scaffolds (Chi-PCL) were compared with the scaffolds fabricated using surface coating technique, where chitosan was coated on the pore wall of PCL scaffolds (C-PCL). The C-PCL exhibited a collective improvement in bioactivities of the stem cell on the scaffold, because of the cell compatible environment provided by the presence of chitosan over the scaffolds interface. The C-PCL showed the enhanced cell attachment and proliferation behavior of the scaffolds along with two-fold increase in hemolysis compatibility compared to Chi-PCL. Furthermore, the compression strength in C-PCL increased by 24.52% and 8.62% increase in total percentage porosity compared to Chi-PCL was attained. Along with this, all the bone markers showed significant upregulation in C-PCL scaffolds, which supported the surface coating technique over the conventional methods, even though the pore size of C-PCL was compromised by 19.98% compared with Chi-PCL.

Keywords

  • Bone tissue engineering,
  • Comparison,
  • PCL-chitosan scaffolds,
  • Porogen leaching,
  • Surface coating method,
  • 3-D scaffolds

References

  1. Abdel-Mohsen et al. (2020) Recommendations for measuring HIV reservoir size in cure-directed clinical trials 26(9) (pp. 1339-1350) https://doi.org/10.1038/s41591-020-1022-1
  2. Abdelrazek et al. (2016) Spectroscopic studies and thermal properties of PCL/PMMA biopolymer blend 3(1) (pp. 10-15) https://doi.org/10.1016/j.ejbas.2015.06.001
  3. Balan and Verestiuc (2014) Strategies to improve chitosan hemocompatibility: a review (pp. 171-188) https://doi.org/10.1016/j.eurpolymj.2014.01.033
  4. Baptista and Guedes (2021) Morphological and mechanical characterization of 3D printed PLA scaffolds with controlled porosity for trabecular bone tissue replacement https://doi.org/10.1016/j.msec.2020.111528
  5. Boido et al. (2019) Chitosan-based hydrogel to support the paracrine activity of mesenchymal stem cells in spinal cord injury treatment 9(1) (pp. 1-16) https://doi.org/10.1038/s41598-019-42848-w
  6. Chen et al. (2000) A biodegradable hybrid sponge nested with collagen microsponges—Chen—2000 51(2) (pp. 273-279) https://doi.org/10.1002/(sici)1097-4636(200008)51:2<273::aid-jbm16>3.0.co;2-o
  7. Cipitria et al. (2011) Design, fabrication and characterization of PCL electrospun scaffolds—a review 21(26) (pp. 9419-9453) https://doi.org/10.1039/c0jm04502k
  8. Depan et al. (2011) Organic/inorganic hybrid network structure nanocomposite scaffolds based on grafted chitosan for tissue engineering 7(5) (pp. 2163-2175) https://doi.org/10.1016/j.actbio.2011.01.029
  9. Dhandayuthapani et al. (2011) Polymeric scaffolds in tissue engineering application: a review https://doi.org/10.1155/2011/290602
  10. Distler et al. (2020) Polymer-bioactive glass composite filaments for 3D scaffold manufacturing by fused deposition modeling: fabrication and characterization https://doi.org/10.3389/fbioe.2020.00552
  11. Duceppe and Tabrizian (2010) Advances in using chitosan-based nanoparticles for in vitro and in vivo drug and gene delivery 7(10) (pp. 1191-1207) https://doi.org/10.1517/17425247.2010.514604
  12. Dutta et al. (2021) 3D-printed bioactive and biodegradable hydrogel scaffolds of alginate/gelatin/cellulose nanocrystals for tissue engineering (pp. 644-658) https://doi.org/10.1016/j.ijbiomac.2020.12.011
  13. Esbah Tabaei et al. (2021) Combinatorial effects of coral addition and plasma treatment on the properties of chitosan/polyethylene oxide nanofibers intended for bone tissue engineering https://doi.org/10.1016/j.carbpol.2020.117211
  14. Farzinfar and Paydayesh (2019) Investigation of polyvinyl alcohol nanocomposite hydrogels containing chitosan nanoparticles as wound dressing 68(11) (pp. 628-638) https://doi.org/10.1080/00914037.2018.1482463
  15. Gao et al. (2016) Polydopamine-templated hydroxyapatite reinforced polycaprolactone composite nanofibers with enhanced cytocompatibility and osteogenesis for bone tissue engineering 8(5) (pp. 3499-3515) https://doi.org/10.1021/acsami.5b12413
  16. Gautam et al. (2014) Fabrication and characterization of PCL/gelatin/chitosan ternary nanofibrous composite scaffold for tissue engineering applications 49(3) (pp. 1076-1089) https://doi.org/10.1007/s10853-013-7785-8
  17. Ghorbani et al. (2016) Effect of hydroxyapatite nano-particles on morphology, rheology and thermal behavior of poly(caprolactone)/chitosan blends (pp. 980-989) https://doi.org/10.1016/j.msec.2015.10.076
  18. Ghosal et al. (2017) Structural and surface compatibility study of modified electrospun poly(ε-caprolactone) (PCL) composites for skin tissue engineering 18(1) (pp. 72-81) https://doi.org/10.1208/s12249-016-0500-8
  19. Grigoriadou et al. (2014) Evaluation of silica-nanotubes and strontium hydroxyapatite nanorods as appropriate nanoadditives for poly(butylene succinate) biodegradable polyester for biomedical applications (pp. 49-59) https://doi.org/10.1016/j.compositesb.2013.12.015
  20. Gu et al. (2013) Molecular simulation to predict miscibility and phase separation behavior of chitosan/poly(ε-caprolactone) binary blends: a comparison with experiments 22(7) (pp. 377-384) https://doi.org/10.1002/mats.201300109
  21. Habiba et al. (2018) Adsorption study of methyl orange by chitosan/polyvinyl alcohol/zeolite electrospun composite nanofibrous membrane (pp. 79-85) https://doi.org/10.1016/j.carbpol.2018.02.081
  22. Jain et al. (2015) Culture & differentiation of mesenchymal stem cell into osteoblast on degradable biomedical composite scaffold: in vitro study 142(6) (pp. 747-758) https://doi.org/10.4103/0971-5916.174568
  23. Jiao et al. (2007) Fabrication and characterization of PLLA-chitosan hybrid scaffolds with improved cell compatibility 80(4) (pp. 820-825) https://doi.org/10.1002/jbm.a.31061
  24. Kane and Roeder (2012) Effects of hydroxyapatite reinforcement on the architecture and mechanical properties of freeze-dried collagen scaffolds (pp. 41-49) https://doi.org/10.1016/j.jmbbm.2011.09.010
  25. Khorramnezhad et al. (2021) Effect of surface modification on physical and cellular properties of PCL thin film https://doi.org/10.1016/j.colsurfb.2021.111582
  26. Kim and Kim (2014) Three-dimensional electrospun polycaprolactone (PCL)/alginate hybrid composite scaffolds (pp. 213-221) https://doi.org/10.1016/j.carbpol.2014.08.008
  27. Li et al. (2010) A one-step method to fabricate PLLA scaffolds with deposition of bioactive hydroxyapatite and collagen using ice-based microporogens 6(6) (pp. 2013-2019) https://doi.org/10.1016/j.actbio.2009.12.008
  28. Li et al. (2012) Fabrication of poly(lactide-co-glycolide) scaffold embedded spatially with hydroxyapatite particles on pore walls for bone tissue engineering 23(11) (pp. 1446-1453) https://doi.org/10.1002/pat.2066
  29. Madihally and Matthew (1999) Porous chitosan scaffolds for tissue engineering 20(12) (pp. 1133-1142) https://doi.org/10.1016/S0142-9612(99)00011-3
  30. Maharjan et al. (2021) Regenerated cellulose nanofiber reinforced chitosan hydrogel scaffolds for bone tissue engineering https://doi.org/10.1016/j.carbpol.2020.117023
  31. Martino et al. (2011) Novative biomaterials based on chitosan and poly(ε-caprolactone): elaboration of porous structures 19(4) (pp. 819-826) https://doi.org/10.1007/s10924-011-0354-9
  32. Mathews et al. (2008) Vascular cell viability on polyvinyl alcohol hydrogels modified with water-soluble and -insoluble chitosan 84(2) (pp. 531-540) https://doi.org/10.1002/jbm.b.30901
  33. Midha et al. (2021) Tissue-specific mesenchymal stem cell-dependent osteogenesis in highly porous chitosan-based bone analogs 10(2) (pp. 303-319) https://doi.org/10.1002/sctm.19-0385
  34. Miszuk et al. (2021) Elastic mineralized 3D electrospun PCL nanofibrous scaffold for drug release and bone tissue engineering 4(4) (pp. 3639-3648) https://doi.org/10.1021/acsabm.1c00134
  35. Murphy and O’Brien (2010) Understanding the effect of mean pore size on cell activity in collagen-glycosaminoglycan scaffolds 4(3) (pp. 377-381) https://doi.org/10.4161/cam.4.3.11747
  36. Naahidi et al. (2017) Biocompatibility of hydrogel-based scaffolds for tissue engineering applications 35(5) (pp. 530-544) https://doi.org/10.1016/j.biotechadv.2017.05.006
  37. Nahanmoghadam et al. (2021) Design and fabrication of bone tissue scaffolds based on PCL/PHBV containing hydroxyapatite nanoparticles: dual-leaching technique 109(6) (pp. 981-993) https://doi.org/10.1002/jbm.a.37087
  38. Nair and Laurencin (2007) Biodegradable polymers as biomaterials 32(8–9) (pp. 762-798) https://doi.org/10.1016/j.progpolymsci.2007.05.017
  39. Nandy et al. (2014) Fibroblast growth factor-2 alone as an efficient inducer for differentiation of human bone marrow mesenchymal stem cells into dopaminergic neurons 21(1) (pp. 1-10) https://doi.org/10.1186/s12929-014-0083-1
  40. Panas-Perez et al. (2013) Development of a silk and collagen fiber scaffold for anterior cruciate ligament reconstruction 24(1) (pp. 257-265) https://doi.org/10.1007/s10856-012-4781-5
  41. Perumal et al. (2020) Synthesis of magnesium phosphate nanoflakes and its PCL composite electrospun nanofiber scaffolds for bone tissue regeneration https://doi.org/10.1016/j.msec.2019.110527
  42. Pires et al. (2018) Crystallization kinetics of PCL and PCL–glass composites for additive manufacturing 134(3) (pp. 2115-2125) https://doi.org/10.1007/s10973-018-7307-7
  43. Poddar et al. (2021) Influence of varying concentrations of chitosan coating on the pore wall of polycaprolactone based porous scaffolds for tissue engineering application https://doi.org/10.1016/j.carbpol.2020.117501
  44. Raftery et al. (2015) Development of a gene-activated scaffold platform for tissue engineering applications using chitosan-pDNA nanoparticles on collagen-based scaffolds (pp. 84-94) https://doi.org/10.1016/j.jconrel.2015.05.005
  45. Reyna-Urrutia et al. (2019) Effect of two crosslinking methods on the physicochemical and biological properties of the collagen-chitosan scaffolds (pp. 424-433) https://doi.org/10.1016/j.eurpolymj.2019.05.010
  46. Rodrigues et al. (2020) Mechanical, thermal and antimicrobial properties of chitosan-based-nanocomposite with potential applications for food packaging 28(4) (pp. 1216-1236) https://doi.org/10.1007/s10924-020-01678-y
  47. Rodrigues et al. (2021) A novel technique to produce tubular scaffolds based on collagen and elastin 45(5) (pp. E113-E122) https://doi.org/10.1111/aor.13857
  48. Saito et al. (2015) Biomineral coating increases bone formation by ex vivo BMP-7 gene therapy in rapid prototyped poly(l-lactic acid) (PLLA) and poly(ε-caprolactone) (PCL) porous scaffolds 4(4) (pp. 621-632) https://doi.org/10.1002/adhm.201400424
  49. Sarasam et al. (2006) Blending chitosan with polycaprolactone: effects on physicochemical and antibacterial properties 7(4) (pp. 1131-1138) https://doi.org/10.1021/bm050935d
  50. Sari et al. (2021) Porous structure of bioceramics carbonated hydroxyapatite-based honeycomb scaffold for bone tissue engineering https://doi.org/10.1016/j.mtcomm.2021.102135
  51. Saroia et al. (2018) A review on biocompatibility nature of hydrogels with 3D printing techniques, tissue engineering application and its future prospective 1(4) (pp. 265-279) https://doi.org/10.1007/s42242-018-0029-7
  52. Shelma and Sharma (2011) Development of lauroyl sulfated chitosan for enhancing hemocompatibility of chitosan 84(2) (pp. 561-570) https://doi.org/10.1016/j.colsurfb.2011.02.018
  53. Shkarina et al. (2018) 3D biodegradable scaffolds of polycaprolactone with silicate-containing hydroxyapatite microparticles for bone tissue engineering: High-resolution tomography and in vitro study 8(1) (pp. 1-13) https://doi.org/10.1038/s41598-018-27097-7
  54. Siddiqui et al. (2021) Electropsun polycaprolactone fibres in bone tissue engineering: a review 63(5) (pp. 363-388) https://doi.org/10.1007/s12033-021-00311-0
  55. Simão et al. (2017) Thermal properties and crystallinity of PCL/PBSA/cellulose nanocrystals grafted with PCL chains https://doi.org/10.1002/app.44493
  56. Vaidhyanathan et al. (2021) Fabrication and investigation of the suitability of chitosan-silver composite scaffolds for bone tissue engineering applications (pp. 178-187) https://doi.org/10.1016/j.procbio.2020.10.008
  57. Wei et al. (2006) Nano-fibrous scaffold for controlled delivery of recombinant human PDGF-BB 112(1) (pp. 103-110) https://doi.org/10.1016/j.jconrel.2006.01.011
  58. Wu et al. (2017) Reconstruction of large-scale defects with a novel hybrid scaffold made from poly(l-lactic acid)/nanohydroxyapatite/alendronate-loaded chitosan microsphere: in vitro and in vivo studies 7(1) (pp. 1-14) https://doi.org/10.1038/s41598-017-00506-z
  59. Xia et al. (2017) A review of gradient stiffness hydrogels used in tissue engineering and regenerative medicine 105(6) (pp. 1799-1812) https://doi.org/10.1002/jbm.a.36034
  60. Xing et al. (2019) Covalently polysaccharide-based alginate/chitosan hydrogel embedded alginate microspheres for BSA encapsulation and soft tissue engineering (pp. 340-348) https://doi.org/10.1016/j.ijbiomac.2019.01.065
  61. Zarrintaj et al. (2018) Agarose-based biomaterials for tissue engineering (pp. 66-84) https://doi.org/10.1016/j.carbpol.2018.01.060
  62. Zhao et al. (2018) Enhanced interfacial adhesion by reactive carbon nanotubes: new route to high-performance immiscible polymer blend nanocomposites with simultaneously enhanced toughness, tensile strength, and electrical conductivity 10(10) (pp. 8411-8416) https://doi.org/10.1021/acsami.8b01704