10.1007/s40204-021-00171-6

Biological and bioactivity assessment of dextran nanocomposite hydrogel for bone regeneration

  • Parisa Nikpour1,
  • Hamed Salimi-Kenari*1,
  • Sayed Mahmood Rabiee2,
  1. Department of Chemical Engineering, Faculty of Engineering and Technology, University of Mazandaran, Babolsar, IR
  2. Department of Materials Engineering, Faculty of Mechanical Engineering, Babol Noshirvani University of Technology, Babol, IR

Published in Issue 2021-11-01

How to Cite

Nikpour, P., Salimi-Kenari, H., & Rabiee, S. M. (2021). Biological and bioactivity assessment of dextran nanocomposite hydrogel for bone regeneration. Progress in Biomaterials, 10(4 (December 2021). https://doi.org/10.1007/s40204-021-00171-6
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Abstract

Abstract Insufficient biological and bioactive properties of dextran hydrogels limit their applications as promising scaffolds for tissue engineering. We developed nanocomposite dextran hydrogels comprised of bioactive glass (nBGC: 64% SiO2, 31% CaO, 5% P 2 O 5 ) nanoparticles with an average particle size of 77 nm using a chemical crosslinking of dextran chains to form 3D hydrogel networks. In the current study; bioactivity of the obtained nanocomposite hydrogels was evaluated through the formation of apatite crystal structures after the incubation in simulated body fluid (SBF) at various submersion periods and nBGC content. The scanning electron microscopy (SEM) micrographs represented an enhanced hydroxyapatite formation on the cross section of nanocomposite comprising of nBGC content from 2 to 8 (% by wt). Biomineralization results of Dex-8 (% by wt) composite during 7, 14 and 28 days immersion indicated the apatite layer formation and the growth of apatite crystal size on the surface and cross section of the nanocomposite. Moreover, MTT assessments indicated that human osteosarcoma cells (SaOS-2) were able to adhere and spread within the dextran hydrogels reinforced with the bioactive glass nanoparticles. With regard to enhanced bioactivity and biocompatibility, the developed dextran-nBGC hydrogel could be considered as a suitable candidate for bone tissue engineering application.

Keywords

  • Bioactive glass ceramic,
  • Dextran nanocomposite hydrogel,
  • Bioactivity assessment

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