10.1007/s40204-021-00149-4

Tissue engineering of collagen scaffolds crosslinked with plant based polysaccharides

Show Details XML HTML PDF
  • Rohit Rekulapally1,
  • K. Udayachandrika1,
  • Sirisha Hamlipur1,
  • Anuja Sasidharan Nair1,
  • Biswajit Pal1,
  • Shashi Singh*1,
  1. CSIR Centre for Cellular and Molecular Biology, Hyderabad, 500007, IN
Cover Image

Published in Issue 2021-02-18

How to Cite

Rekulapally, R., Udayachandrika, K., Hamlipur, S., Sasidharan Nair, A., Pal, B., & Singh, S. (2021). Tissue engineering of collagen scaffolds crosslinked with plant based polysaccharides. Progress in Biomaterials, 10(1 (March 2021). https://doi.org/10.1007/s40204-021-00149-4
Crossref
Scopus
Google Scholar
Europe PMC

Abstract

Abstract Ideally, a bioscaffold should mimic the characteristics of an extracellular matrix of a living organ of interest. The present study deals with the formation of composite scaffolds of collagen with gum arabic. Collagen was cross-linked with oxidized gum arabic having aldehyde groups to form a porous block. By changing the oxidation level of gum arabic, incorporation of the polysaccharides into the scaffold could be varied resulting in scaffolds with variable polysaccharide to protein content. A series of scaffolds were made by altering collagen concentration and oxidation level of gum arabic. The scaffolds were tested for their physical properties, stability, biocompatibility and ability to support the cell growth. Results implied that variable polysaccharide incorporation into the scaffolds was possible depending on the oxidation level of gum arabic which could influence the swelling behavior. The scaffolds showed non-toxic behavior towards the mesenchymal stem cells and nucleus pulposa cells using viability assay in culture conditions up to 30 days; the growth of cells was seen at all combinations of gels. Nucleus pulposa cells were able to maintain their phenotype in the GACO gels. The studies show that these scaffolds are potential candidates in applications, such as tissue engineering, and can be designed to match the requirement of different cell/tissues as per their ECM.

Keywords

  • Extracellular matrix,
  • Cross-linked collagen,
  • Polysaccharides,
  • Biocompatibility,
  • Cytotoxicity,
  • Differentiation
HTML PDF

References

  1. Ali et al. (2009) Biological effects of gum arabic: a review of some recent research (pp. 1-8) https://doi.org/10.1016/j.fct.2008.07.001
  2. Ali et al. (2018) A new study of iodine complexes of oxidized gum arabic: an interaction between iodine monochloride and aldehyde groups (pp. 337-347) https://doi.org/10.1016/j.carbpol.2017.10.005
  3. Bačáková et al. (2014) Polysaccharides as cell carriers for tissue engineering: the use of cellulose in vascular wall reconstruction (pp. s29-s47) https://doi.org/10.33549/physiolres.932644
  4. Camponeschi et al. (2015) New formulations of polysaccharide-based hydrogels for drug release and tissue engineering (pp. 3-23) https://doi.org/10.3390/gels1010003
  5. Davidenko et al. (2015) Control of crosslinking for tailoring collagen-based scaffolds stability and mechanics https://doi.org/10.1016/j.actbio.2015.07.034
  6. Edgar et al. (2016) Heterogeneity of scaffold biomaterials in tissue engineering https://doi.org/10.3390/ma9050332
  7. Ehrenfreund-Kleinman et al. (2002) Synthesis and biodegradation of arabinogalactan sponges prepared by reductive amination https://doi.org/10.1016/S0142-9612(02)00209-0
  8. Frantz et al. (2010) The extracellular matrix at a glance https://doi.org/10.1242/jcs.023820
  9. Glowacki and Mizuno (2007) Collagen scaffolds for tissue engineering 89(338–344)
  10. Ibekwe et al. (2017) Synthesis and characterization of chitosan/gum arabic nanoparticles for bone regeneration (pp. 28-36) https://doi.org/10.12691/ajmse-5-1-4
  11. Idris et al. (1998) Characterization of gum from Acacia senegal trees of different age and location using multidetection gel permeation chromatography (pp. 379-388) https://doi.org/10.1016/S0268-005X(98)00058-7
  12. Lapidot et al. (2012) Clues for biomimetics from natural composite materials (pp. 1409-1423) https://doi.org/10.2217/nnm.12.107
  13. Li et al. (2008) Fabrication of bioceramic scaffolds with pre-designed internal architecture by gel casting and indirect stereolithography techniques https://doi.org/10.1007/s10934-007-9148-9
  14. Mano et al. (2007) Natural origin biodegradable systems in tissue engineering and regenerative medicine: present status and some moving trends (pp. 999-1030) https://doi.org/10.1098/rsif.2007.0220
  15. Mwale et al. (2004) Distinction between the extracellular matrix of the nucleus pulposus and hyaline cartilage: a requisite for tissue engineering of intervertebral disc (pp. 58-63) https://doi.org/10.22203/eCM.v008a06
  16. Nishi and Jayakrishnan (2005) Preparation and in vitro evaluation of primaquine-conjugated gum arabic microspheres (pp. 191-197)
  17. O'Brien (2011) Biomaterials and scaffolds for tissue engineering 14(3) (pp. 88-95) https://doi.org/10.1016/S1369-7021(11)70058-X
  18. Osborne et al. (1998) Grant Investigation into the tensile properties of collagen/chondroitin-6-sulphate gels: the effect of crosslinking agents and diamines https://doi.org/10.1007/BF02522870
  19. Palma et al. (2015) Covalent coupling of gum arabic onto superparamagnetic iron oxide nanoparticles for MRI cell labeling: physicochemical and in vitro characterization (pp. 320-328) https://doi.org/10.1002/cmmi.1635
  20. Rodrigues et al. (2012) Synergistic effect of scaffold composition and dynamic culturing environment in multilayered systems for bone tissue engineering (pp. 24-30) https://doi.org/10.1002/term.499
  21. Sarika and James (2015) Preparation and characterisation of gelatin–gum arabic aldehyde nanogels via inverse miniemulsion technique https://doi.org/10.1016/j.ijbiomac.2015.02.038
  22. Sarika et al. (2014) (2014) Modified gum arabic cross-linked gelatin scaffold for biomedical applications (pp. 272-279) https://doi.org/10.1016/j.msec.2014.06.042
  23. Sarika et al. (2016) Microgravity as a means to incorporate HepG2 aggregates in polysaccharide–protein hybrid scaffold https://doi.org/10.1007/s10856-015-5638-5
  24. Shao et al. (2000) Complexation of borate with cross-linked polysaccharide anion exchanger: B NMR and adsorption properties studies https://doi.org/10.1021/ma991257o
  25. Silva et al. (2015) Polysaccharide-based strategies for heart tissue engineering (pp. 267-277) https://doi.org/10.1016/j.carbpol.2014.06.010
  26. Thejaswi et al. (2012) Immune modulatory responses of Mesenchymal stem cells from different sources in cultures and in vivo (pp. 1-13)
  27. Tiwari et al. (2019) Polysaccharide based scaffolds for soft tissue engineering applications https://doi.org/10.3390/polym11010001
  28. Tummalapalli and Gupta (2015) UV–Vis spectrophotometric method for the estimation of aldehyde groups in periodate-oxidized polysaccharides using 2,4-dinitrophenyl hydrazine 34(6) (pp. 338-348) https://doi.org/10.1080/07328303.2015.1068793
  29. Ungaro F, Biondi M, Indolfi L, De Rosa G, La Rotonda MI, Quaglia F, P. Netti P (2005) Bioactivated polymer scaffolds for tissue engineering
  30. .
  31. In: Ashammakhi N, Reis RL (eds) Topics in tissue engineering. 2:1–38
  32. Varkhade and Pawar (2013) Spectrophotometric estimation of total polysaccharides in Plantago ovata husk mucilage
  33. Yanagishita (1993) Function of proteoglycans in the extracellular matrix