Preparing and Synthesizing Modified Electrospun Nanofibers via Online UV Method from Poly Acrylonitrile and Poly Vinyl Alcohol for Enzyme (glucose oxidase) Iimmobilization

Authors

• Sepideh Asadi ¹
• Mehdi Jahanfar * ¹
• Saeed Javadi Anaghizi ²
• Dariush Minai-Tehrani ¹

1. Faculty of Life Sciences and Biotechnology, Shahid Beheshti University, Tehran, Iran
2. Central Laboratory of Shahid Beheshti University, Tehran, Iran

Abstract

The study explores the use of nanofibers for enzyme immobilization, leveraging their high contact area and controlled cavities. By employing ultraviolet (UV) irradiation on a polymeric blend of polyvinyl alcohol (PVA) and poly acrylonitrile (PAN) during electrospinning, nanofibers were fabricated for glucose oxidase (GOD) immobilization. UV treatment enhanced carboxyl group presence on nanofiber surfaces, as confirmed by Fourier transform infrared (FTIR) analysis. Enzyme activity assessments demonstrated a 33% increase in immobilized enzyme activity when PVA content in the nanofibers was raised from 1% to 3%. Scanning electron microscopy (SEM) images revealed that UV treatment helped maintain nano filament structures post-immobilization, aiding enzyme retention and controlled release. Atomic force microscopy (AFM) images displayed increased roughness in UV-treated nanofibers. The study showcased GOD reusability up to 12 cycles in 3% PVA-containing samples.

Keywords

• UV
• PVA
• Enzyme immobilization
• Nanofibers
• Glucose oxidase
• PAN