10.1007/s40097-022-00481-6

Synthesis and characterization of cellulose, β-cyclodextrin, silk fibroin-based hydrogel containing copper-doped cobalt ferrite nanospheres and exploration of its biocompatibility

  • Reza Eivazzadeh-Keihan1,
  • Fatemeh Ganjali1,
  • Hooman Aghamirza Moghim Aliabadi2,
  • Ali Maleki1,
  • Saeedeh Pouri3,
  • Mohammad Mahdavi4,
  • Ahmed Esmail Shalan5,
  • Senentxu Lanceros-Méndez*6,
  1. Catalysts and Organic Synthesis Research Laboratory, Department of Chemistry, Iran University of Science and Technology, Tehran, 16846-13114, IR
  2. Protein Chemistry Laboratory, Department of Medical Biotechnology, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, IR Advanced Chemical Studies Lab, Department of Chemistry, K. N. Toosi University of Technology, Tehran, IR
  3. Protein Chemistry Laboratory, Department of Medical Biotechnology, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, IR
  4. Endocrinology and Metabolism Research Center, Endocrinology and Metabolism Clinical Sciences Institute, Tehran University of Medical Sciences, Tehran, IR
  5. BCMaterials, Basque Center for Materials, Applications and Nanostructures, Martina Casiano, Leioa, 48940, ES Central Metallurgical Research and Development Institute (CMRDI), Helwan, Cairo, 11421, EG
  6. BCMaterials, Basque Center for Materials, Applications and Nanostructures, Martina Casiano, Leioa, 48940, ES Basque Foundation for Science, IKERBASQUE, Bilbao, 48009, ES

Published in Issue 01-03-2022

How to Cite

Eivazzadeh-Keihan, R., Ganjali, F., Aliabadi, H. A. M., Maleki, A., Pouri, S., Mahdavi, M., Shalan, A. E., & Lanceros-Méndez, S. (2022). Synthesis and characterization of cellulose, β-cyclodextrin, silk fibroin-based hydrogel containing copper-doped cobalt ferrite nanospheres and exploration of its biocompatibility. Journal of Nanostructure in Chemistry, 13(1 (February 2023). https://doi.org/10.1007/s40097-022-00481-6
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Abstract

Abstract A novel multifunctional biocomposite scaffold based on cellulose (Cel) and β-cyclodextrin (β.CD) biopolymers modified with extracted silk fibroin (SF) as well as magnetic copper-doped cobalt ferrite (CuCoFe 2 O 4 ) nanospheres has been developed. The hybrid scaffold was characterized by FE–SEM, TGA, FT–IR, and EDS techniques to demonstrate the core–shell morphology, thermal stability of the main structure, the chemical bonds as well as the good connections in the composite structure, and the good distribution of the particles in the formed structure, respectively. The Cel-β.CD/SF/CuCoFe 2 O 4 biocomposite shows a cell viability above 84% in addition to 80% after three and seven days, respectively, and low hemolytic effect (below 3%), which confirms a high hemocompatibility of this hydrogel. Further, the antibacterial property of the biocomposite was identified from superficial P. aeruginosa biofilm formation prevention. Thus, developed biocompatible hydrogel shows suitable characteristics for a variety of biomedical applications. Graphical abstract

Keywords

  • β-Cyclodextrin,
  • Cellulose,
  • Copper-doped cobalt ferrite,
  • Silk fibroin,
  • Anti-bacterial,
  • Cell viability

References

  1. Ahmadi et al. (2020) Preparation and in-vitro evaluation of pH-responsive cationic cyclodextrin coated magnetic nanoparticles for delivery of methotrexate to the Saos-2 bone cancer cells https://doi.org/10.1016/j.jddst.2020.101584
  2. Ashour et al. (2018) Antimicrobial activity of metal-substituted cobalt ferrite nanoparticles synthesized by sol–gel technique (pp. 141-151) https://doi.org/10.1016/j.partic.2017.12.001
  3. Bae et al. (2021) Dual-crosslinked silk fibroin hydrogels with elasticity and cytocompatibility for the regeneration of articular cartilage https://doi.org/10.1016/j.polymer.2021.123739
  4. Bilal et al. (2019) Agarose-chitosan hydrogel-immobilized horseradish peroxidase with sustainable bio-catalytic and dye degradation properties (pp. 742-749) https://doi.org/10.1016/j.ijbiomac.2018.11.220
  5. Bucciarelli et al. (2021) A design of experiment rational optimization of the degumming process and its impact on the silk fibroin properties (pp. 1374-1393) https://doi.org/10.1021/acsbiomaterials.0c01657
  6. Chenab et al. (2019) Biomedical applications of nanoflares: targeted intracellular fluorescence probes (pp. 342-358) https://doi.org/10.1016/j.nano.2019.02.006
  7. Chouhan et al. (2018) In situ forming injectable silk fibroin hydrogel promotes skin regeneration in full thickness burn wounds https://doi.org/10.1002/adhm.201801092
  8. Eivazzadeh-Keihan et al. (2021) Investigation of the biological activity, mechanical properties and wound healing application of a novel scaffold based on lignin–agarose hydrogel and silk fibroin embedded zinc chromite nanoparticles (pp. 17914-17923) https://doi.org/10.1039/D1RA01300A
  9. Eivazzadeh-Keihan et al. (2021) Magnetic copper ferrite nanoparticles functionalized by aromatic polyamide chains for hyperthermia applications (pp. 8847-8854) https://doi.org/10.1021/acs.langmuir.1c01251
  10. Eivazzadeh-Keihan et al. (2020) Recent advances in the application of mesoporous silica-based nanomaterials for bone tissue engineering https://doi.org/10.1016/j.msec.2019.110267
  11. Eivazzadeh-Keihan et al. (2020) Alginate hydrogel-polyvinyl alcohol/silk fibroin/magnesium hydroxide nanorods: a novel scaffold with biological and antibacterial activity and improved mechanical properties (pp. 1959-1971) https://doi.org/10.1016/j.ijbiomac.2020.08.090
  12. Eivazzadeh-Keihan et al. (2018) Recent advances on nanomaterial based electrochemical and optical aptasensors for detection of cancer biomarkers (pp. 103-115) https://doi.org/10.1016/j.trac.2017.12.019
  13. Eivazzadeh-Keihan et al. (2021) Chitosan hydrogel/silk fibroin/Mg (OH) 2 nanobiocomposite as a novel scaffold with antimicrobial activity and improved mechanical properties (pp. 1-13) https://doi.org/10.1038/s41598-020-80133-3
  14. Eivazzadeh-Keihan et al. (2020) Graphene oxide/alginate/silk fibroin composite as a novel bionanostructure with improved blood compatibility, less toxicity and enhanced mechanical properties https://doi.org/10.1016/j.carbpol.2020.116802
  15. Eivazzadeh-Keihan et al. (2019) A novel biocompatible core-shell magnetic nanocomposite based on cross-linked chitosan hydrogels for in vitro hyperthermia of cancer therapy (pp. 407-414) https://doi.org/10.1016/j.ijbiomac.2019.08.031
  16. Ha et al. (2005) Structural studies of bombyx m ori silk fibroin during regeneration from solutions and wet fiber spinning (pp. 1722-1731) https://doi.org/10.1021/bm050010y
  17. Haney et al. (2018) Critical assessment of methods to quantify biofilm growth and evaluate antibiofilm activity of host defence peptides https://doi.org/10.3390/biom8020029
  18. He et al. (2019) Heparinized silk fibroin hydrogels loading FGF1 promote the wound healing in rats with full-thickness skin excision (pp. 1-12) https://doi.org/10.1186/s12938-019-0716-4
  19. Hernández-González et al. (2020) Alginate hydrogels for bone tissue engineering, from injectables to bioprinting: A review https://doi.org/10.1016/j.carbpol.2019.115514
  20. Hu et al. (2019) Advances in crosslinking strategies of biomedical hydrogels (pp. 843-855) https://doi.org/10.1039/C8BM01246F
  21. Huang et al. (2019) Multifunctional polypyrrole-silver coated layered double hydroxides embedded into a biodegradable polymer matrix for enhanced antibacterial and gas barrier properties (pp. 231-241)
  22. Huang et al. (2018) On-demand dissolvable selfhealing hydrogel based on carboxymethyl chitosan and cellulose nanocrystal for deep partial thickness burn wound healing (pp. 41076-41088) https://doi.org/10.1021/acsami.8b14526
  23. Ijaz et al. (2020) A review on antibacterial properties of biologically synthesized zinc oxide nanostructures (pp. 2815-2826) https://doi.org/10.1007/s10904-020-01603-9
  24. Johari et al. (2020) Tuning the conformation and mechanical properties of silk fibroin hydrogels https://doi.org/10.1016/j.eurpolymj.2020.109842
  25. Kamel et al. (2020) Nanofibrillated cellulose/cyclodextrin based 3D scaffolds loaded with raloxifene hydrochloride for bone regeneration (pp. 704-716) https://doi.org/10.1016/j.ijbiomac.2020.04.019
  26. Kaniewska et al. (2020) Nanocomposite hydrogel films and coatings–Features and applications https://doi.org/10.1016/j.apmt.2020.100776
  27. Koopmans and Ritter (2007) Color change of N-isopropylacrylamide copolymer bearing reichardts dye as optical sensor for lower critical solution temperature and for host− guest interaction with β- cyclodextrin (pp. 3502-3503) https://doi.org/10.1021/ja068959v
  28. Kundu, A., Hasnain, M.S., Nayak, A.K.: Chitosan-based hydrogels in drug delivery. In: Chitosan in Drug Delivery. pp. 361–387. Elsevier (2022).
  29. Liu et al. (2021) Film-like bacterial cellulose/cyclodextrin oligomer composites with controllable structure for the removal of various persistent organic pollutants from water https://doi.org/10.1016/j.jhazmat.2020.124122
  30. Liu et al. (2016) Characterization and behavior of composite hydrogel prepared from bamboo shoot cellulose and β-cyclodextrin (pp. 527-534) https://doi.org/10.1016/j.ijbiomac.2016.05.023
  31. Mahinroosta et al. (2018) Hydrogels as intelligent materials: a brief review of synthesis, properties and applications (pp. 42-55) https://doi.org/10.1016/j.mtchem.2018.02.004
  32. Palantöken et al. (2020) Cellulose hydrogels physically crosslinked by glycine: Synthesis, characterization, thermal and mechanical properties https://doi.org/10.1002/app.48380
  33. Polat et al. (2020) Agar/κ-carrageenan/montmorillonite nanocomposite hydrogels for wound dressing applications (pp. 4591-4602) https://doi.org/10.1016/j.ijbiomac.2020.09.048
  34. Qin et al. (2019) A hydrogel directly assembled from a copper metal–organic polyhedron for antimicrobial application (pp. 2206-2209)
  35. Rahmani Del Bakhshayesh et al. (2018) Recent advances on biomedical applications of scaffolds in wound healing and dermal tissue engineering (pp. 691-705) https://doi.org/10.1080/21691401.2017.1349778
  36. Rasoulzadeh and Namazi (2017) Carboxymethyl cellulose/graphene oxide bio-nanocomposite hydrogel beads as anticancer drug carrier agent (pp. 320-326) https://doi.org/10.1016/j.carbpol.2017.03.014
  37. Rastogi and Kandasubramanian (2020) Processing trends of silk fibers: Silk degumming, regeneration and physical functionalization (pp. 1794-1810) https://doi.org/10.1080/00405000.2020.1727269
  38. Ren et al. (2020) Leveraging metal oxide nanoparticles for bacteria tracing and eradicating https://doi.org/10.1002/VIW.20200052
  39. Salleh et al. (2019) Superabsorbent hydrogel from oil palm empty fruit bunch cellulose and sodium carboxymethylcellulose (pp. 50-59) https://doi.org/10.1016/j.ijbiomac.2019.03.028
  40. Sharma and Tiwari (2020) A review on biomacromolecular hydrogel classification and its applications (pp. 737-747) https://doi.org/10.1016/j.ijbiomac.2020.06.110
  41. Patwa et al. (2018) Silk nano-discs: a natural material for cancer therapy https://doi.org/10.1002/bip.23231
  42. Shen et al. (2021) Fabrication of a water-retaining, slow-release fertilizer based on nanocomposite double-network hydrogels via ion-crosslinking and free radical polymerization (pp. 375-382) https://doi.org/10.1016/j.jiec.2020.10.014
  43. Shi et al. (2019) Cobaltmediated multi-functional dressings promote bacteria-infected wound healing (pp. 465-479) https://doi.org/10.1016/j.actbio.2018.12.048
  44. Shitrit and Bianco-Peled (2021) Insights into the formation mechanisms and properties of pectin hydrogel physically cross-linked with chitosan nanogels https://doi.org/10.1016/j.carbpol.2021.118274
  45. Alikhanzadeh-Arani et al. (2021) CoNiZn and CoNiFe nanoparticles: synthesis, physical characterization, and in vitro cytotoxicity evaluations https://doi.org/10.3390/app11125339
  46. Sun et al. (2022) Bioadhesive catechol-modified chitosan wound healing hydrogel dressings through glow discharge plasma technique https://doi.org/10.1016/j.cej.2021.130843
  47. Sun et al. (2020) Preparation and properties of self-healable and conductive PVA-agar hydrogel with ultra-high mechanical strength https://doi.org/10.1016/j.eurpolymj.2019.109465
  48. Taheri-Ledari et al. (2020) Facile route to synthesize Fe 3 O 4@ acacia–SO 3 H nanocomposite as a heterogeneous magnetic system for catalytic applications (pp. 40055-40067) https://doi.org/10.1039/D0RA07986C
  49. Hasani et al. (2020) The efficacy of sono-electro- Fenton process for removal of Cefixime antibiotic from aqueous solutions by response surface methodology (RSM) and evaluation of toxicity of effluent by microorganisms (pp. 6122-6139) https://doi.org/10.1016/j.arabjc.2020.05.012
  50. Zavareh et al. (2020) Chitosan/carbon quantum dot/aptamer complex as a potential anticancer drug delivery system towards the release of 5-fluorouracil (pp. 1422-1430) https://doi.org/10.1016/j.ijbiomac.2020.09.166
  51. Tao et al. (2019) Design and performance of sericin/poly (vinyl alcohol) hydrogel as a drug delivery carrier for potential wound dressing application (pp. 341-351) https://doi.org/10.1016/j.msec.2019.03.111
  52. Tufani et al. (2021) Iron oxide nanoparticles based magnetic luminescent quantum dots (MQDs) synthesis and biomedical/biological applications: a review https://doi.org/10.1016/j.msec.2020.111545
  53. Yang et al. (2022) Mussel-inspired adhesive antioxidant antibacterial hemostatic composite hydrogel wound dressing via photo-polymerization for infected skin wound healing (pp. 341-354) https://doi.org/10.1016/j.bioactmat.2021.06.014
  54. Ye et al. (2018) Robust anisotropic cellulose hydrogels fabricated via strong self-aggregation forces for cardiomyocytes unidirectional growth (pp. 5175-5183) https://doi.org/10.1021/acs.chemmater.8b01799
  55. Zhang et al. (2020) Responsive drug-delivery microcarriers based on the silk fibroin inverse opal scaffolds for controllable drug release https://doi.org/10.1016/j.apmt.2019.100540
  56. Balavijayalakshmi et al. (2012) Influence of copper on the magnetic properties of cobalt ferrite nano particles (pp. 52-54) https://doi.org/10.1016/j.matlet.2012.04.076
  57. Rajabi et al. (2022) Enhanced activation of persulfate by CuCoFe2O4@ MC/AC as a novel nanomagnetic heterogeneous catalyst with ultrasonic for metronidazole degradation https://doi.org/10.1016/j.chemosphere.2021.131872
  58. Keshk and Hamdy (2019) Preparation and physicochemical characterization of zinc oxide/sodium cellulose composite for food packaging (pp. 94-105) https://doi.org/10.3906/kim-1803-83
  59. Kaur et al. (2019) Synthesis, characterization and studies on host-guest interactions of inclusion complexes of metformin hydrochloride with β–cyclodextrin (pp. 162-168) https://doi.org/10.1016/j.molliq.2019.02.127
  60. Minoura (1995) Attachment and growth of fibroblast cells on silk fibroin (pp. 511-516) https://doi.org/10.1006/bbrc.1995.1368
  61. Zhang and Pan (2019) Microstructure transitions and dry-wet spinnability of silk fibroin protein from waste silk quilt https://doi.org/10.3390/polym11101622
  62. Moraes et al. (2010) Preparation and characterization of insoluble silk fibroin/chitosan blend films (pp. 719-727) https://doi.org/10.3390/polym2040719
  63. Zhang et al. (2013) Structure and properties of β-cyclodextrin/cellulose hydrogels prepared in NaOH/urea aqueous solution (pp. 386-393) https://doi.org/10.1016/j.carbpol.2012.12.077
  64. Zhang et al. (2019) Inorganic salts induce thermally reversible and anti-freezing cellulose hydrogels (pp. 7366-7370) https://doi.org/10.1002/anie.201902578
  65. Zuluaga-Vélez et al. (2019) Silk fibroin hydrogels from the Colombian silkworm Bombyx mori L: Evaluation of physicochemical properties https://doi.org/10.1371/journal.pone.0213303