Bioconversion of kitchen waste by Pichia kudriavzeii SGP1 (Sudhida Gautam Parihar1)

Authors

• Sudhida G Parihar * ¹
• Ram Dass Gautam ²

1. Department of Zoology, Hansraj College, University of Delhi-110007, India
2. Division of Entomology, ICAR-Indian Agricultural Research Institute, New Delhi-110012, India

Abstract

Purpose: The study was undertaken with a purpose of at source bioconversion of wet kitchen waste.  To accelerate the “Zero Waste Concept” and meet the UN’s Sustainable Development Goals 2015.
Method: Wet waste was mixed with dry paper, cardboard material and a microbial culture was inoculated in the bag. Proper mixing of the material enhanced the process of composting.
Results: Microgold-Y had a pH range of 6.7 to 7.0 with 45 to 58% moisture content, and suitable for plant growth. Germination test using radish (R. sativus) revealed 100% sprouting between 24 to 48 hrs on Microgold-Y (10%) followed with 93.3% on Microgold-Y (20%) as against 96.6 % in sand (control), grown in small containers (5×6 cm) at 20.2 to 20.7 ⁰c and 58 to 65% RH.  Besides, profuse growth and flowering was observed in B. monnieri on Microgold Y while poor growth without flowering in control. Safety evaluation on earthworm, E. fetida revealed the survival of 60% as compared to 95% in control (cow dung). The worms produced undersized cocoons (<2mm) on microgold as compared to control (2.7 to 3.7 mm) and interestingly regained their reproduction on pre-mature microgold.
Conclusion: The yeast was identified as Pichia kudriavzevii determined by the specialists at ICAR-Indian Agricultural Research Institute, New Delhi-12 based on NCBI Blast Result and FASTA Sequence.  The pH and moisture content of the product (Microgold-Y) ranged from 6.7 to 7.0 and moisture ranged from 45 to 58 per cent, which are ideal attributes of good compost recommended for field applications.

Highlights:

• Bioconversion potential of yeast, Pichia kudriavzeii SGP1 NCBI Accession number OM574927.1.
• Weekly kitchen refuge/waste (327 to 640g) of an urban family (4 members) was converted into quality compost (Microgold-Y) in two months.
• The pH (6.9 to 7.0), moisture (45 to 48 %), germination test on Radish (100%) and growth of Bacopa monnieri testified Microgold-Y as good soil amendment for crop productivity.
• Environmental safety test of (Microgold-Y) on earth worm (E. fetida) was safe.

Graphical Abstract

Keywords

• Eisenia fetida
• Sustainable development goals
• Zero waste model
• Microbial inoculum
• Radish germination
• Bacopa monnieri