Bio-synthesis of Copper Oxide nanoparticles using beetle defensive gland extract: Exploring diverse applications
Authors
Abstract
The present study systematically investigates a direct method for synthesising copper oxide nanoparticles (CuONP) utilising the defensive gland secretion of the beetle Luprops tristis Fabricius (L. tristis) using a greener method under microwave irradiation. By employing the unique biological approach of utilising the insect L. tristis, we successfully characterised the CuONP abbreviated as LCuONP through various analytical techniques, including UV-Vis spectrometry, FTIR analysis, SEM, TEM, Zeta potential analysis, and crystalline structure by XRD analysis. These LCuONP display crystalline, irregular spherical shapes with a rugged surface and have an average size of 15 nm. Moreover, we demonstrate that LCuONP-coated electrodes can effectively sense hydrogen peroxide without the need for enzymes, rendering them valuable biosensors. Additionally, LCuONP show dose-dependent antimicrobial activity against Staphylococcus aureus and Klebsiella pneumoniae, indicating their potential as powerful bacterial inhibitors. Notably, LCuONP induce chromosomal abnormalities in Allium cepa root tips, with higher concentrations leading to decreased mitotic indices and increased chromosomal aberrations. Furthermore, our investigation reveals the antioxidative properties of LCuONP and establishes their dose-dependent cytotoxicity against DLA cells. Overall, this study highlights the diverse applications of biosynthesised CuONP, including antimicrobial and antioxidant functions, as well as their potential in biosensing and cancer therapy. CuONP hold significant utility across various fields of life. Thus, synthesising CuONP from insect secretions presents a novel approach, transforming a nuisance pest into a valuable resource in a more environmentally sustainable, economically feasible, and non-toxic synthesis method.
Graphical Abstract
Keywords
References
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