10.57647/jsaeb.2026.0101.04

Taxonomical Studies and High Genetic Diversity in the Glaucium (Papaveraceae)

  1. Young Researchers and Elite club, Ardabil Branch, Islamic Azad University, Ardabil, Iran

Received: 2025-11-17

Accepted: 2025-12-26

Published in Issue 2026-03-31

How to Cite

Taxonomical Studies and High Genetic Diversity in the Glaucium (Papaveraceae). (2026). Journal of Sustainable Agriculture and Environmental Biology, 1(1). https://doi.org/10.57647/jsaeb.2026.0101.04

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Abstract

Central Asia and Atlantic Europe are both the main areas where Glaucium grows. There are four species, four subspecies, two types, and sects available for Glaucium Sect. Acropetala MoryGlaucium itself is a genus that consists of 19 species, eight subspecies, and 16 variants. Identifying species is of great importance in biogeography, biology, ecology, and protection fields. In Iran, there are ten species, four subspecies, and 14 variants of Glaucium. Considering that they are both genetically and physically flexible, it is taxonomically difficult to classify them. In spite of the fact that there are numerous Glaucium species widely distributed in Iran, the details available about their dispersion tendency, genetic diversity, and divergence method have remained meager. Consequently, we have used morphological approaches along with molecular SCoT markers (start codon targeted markers) to examine 90 accessions of seven distinct Glaucium species which have been gathered from different environments of Iran. The aims of this study are as follows: 1) identifying species of Glaucium using molecular SCoT markers, 2) determining these taxa’s genetic structure in Iran, and 3) examining their inter-species relationship. According to the outcomes, the species can be specified employing both SCoT markers and morphological techniques.

Keywords

  • Iran,
  • Glaucium,
  • Morphology,
  • Species Identification,
  • Structure,
  • SCoT (Start Codon Targeted)

References

  1. Alarcón, M., Vargas, P., Sáez, L., Molero, J., Aldasoro, J.J. (2012). Genetic diversity of mountain plants: Two migration episodes of Mediterranean Erodium (Geraniaceae) Molecular Phylogenetics and Evolution 63: 866–876. https://doi.org/10.3372/wi.45.45301
  2. Al-Quran, S. (2008). Taxonomical and pharmacological survey of therapeutic plants in Jordan. Journal of Natural Products, l (1):10-26. https://doi.org/ 10.1556/034.59.2017.3-4.3
  3. Collard, B. C., & Mackill, D. J. (2009). Start codon targeted (SCoT) polymorphism: a simple, novel DNA marker technique for generating gene-targeted markers in plants. Plant molecular biology reporter, 27(1), 86-93.
  4. Cullen, J., (1966). Glaucium. In: Rechinger, K. H. (ed.), Flora Iranica 34, 2 –7. Akad. Druck- und Verlagsanstalt.
  5. Esfandani Bozchaloyi, S., Sheidai, M., Keshavarzi, M. & Noormohammadi, Z. (2017a) Genetic diversity and morphological variability in Geranium purpureum Vill. (Geraniaceae) Of Iran. Genetika 49: 543–557. https://doi.org/10.2298/GENSR1702543B
  6. Esfandani Bozchaloyi, S., Sheidai, M., Keshavarzi, M. & Noormohammadi, Z. (2017b) Species delimitation in Geranium Sect. Batrachioidea: Morphological and molecular. Acta Botanica Hungarica 59 (3–4): 319–334. https://doi.org/10.1556/034.59.2017.3-4.3
  7. Esfandani Bozchaloyi, S., Sheidai, M., Keshavarzi, M. & Noormohammadi, Z. (2017c) Genetic and morphological diversity in Geranium dissectum (Sec. Dissecta, Geraniaceae) populations. Biologia 72 (10): 1121–1130. https://doi.org/10.1515/biolog-2017-0124
  8. Esfandani Bozchaloyi, S., Sheidai, M., Keshavarzi, M. & Noormohammadi, Z. (2017d) Analysis of genetic diversity in Geranium robertianum by ISSR markers. Phytologia Balcanica 23 (2):157–166.
  9. Esfandani-Bozchaloyi, S. & Sheidai, M. (2019) Comparison Of Dna Extraction Methods From Geranium (Geraniaceae). Acta Botanica Hungarica 61 (3–4): 251–266. https://doi.org/10.1556/034.61.2019.3-4.3
  10. Evanno, G., Regnaut, S. & Goudet, J. (2005) Detecting the number of clusters of individuals using the software STRUCTURE: a simulation study. Molecular Ecology 14: 2611–2620. https://doi.org/10.1111/j.1365-294X.2005.02553.x
  11. Freeland, J.R., Kirk, H. & Peterson, S.D. (2011) Molecular Ecology (2nded). Wiley-Blackwell, UK, 449 pp. https://doi.org/10.1002/9780470979365
  12. Ernest, W. R. (1962) A comparative morphology of Papaveraceae, PhD Dissertation. Standford University, Standford, California
  13. Frichot, E., Schoville, S.D., Bouchard, G., Francois, O. (2013). Testing for associations between loci and environmental gradients using latent factor mixed models. Molecular Biology and Evolution 30: 1687–1699.
  14. Gran, A., Sharifnia, F. (2008). Micro–macrophological studies of the genus Glaucium (Papaveraceae) in Iran. The Iranian Journal of Botany 14: 22–38.
  15. Hoot, S. B., Kadereit, J. W., Blattner, F. R., Jork, K. B., Schwarzbach, A. E., Crane, P. R. (1997). Data congruence of the Papaveraceae s. 1. based on four data sets: atpB and rbcLsequences, trnK restriction sites, and morphological characters. Systematic Botany 22: 575–590.
  16. Hedrick, P.W. (2005). A standardized genetic differentiation measure. Evolution 59:1633−1638.Huson, D.H. & D. Bryant (2006). Application of Phylogenetic Networks in Evolutionary Studies. Mol. Biol. Evol. 23: 254−267.
  17. Jost, L. (2008). GST and its relatives do not measure differentiation. Mol. Ecol. 17: 4015−4026.
  18. Kadereit, J. W. (1993). Glaucium. In: Kubitzki, K. Rohwer, J. C., Bittrichotteidedelberg (eds.), The families and Genera of Vascular Plants, 1–663. Springer Verlag, Berlin.
  19. Kadereit, J. W., Blattner, F. R., Jork, K. B., Schwarzbach, A. E. (1994). Phylogenetic analysis of the Papaverceae s. 1. (including Fumariaceae, Hypecoaceae and Pteridophyllum) based on morphological characters. Botanische Jahrbücher für Systematik und Pflanzengeographie 116: 361–390.
  20. Kiliç, F. M., Yildiz, K., Batir, M. B., Kilic, M., & Büyük, I. (2019). Morphological, palynological and phylogenetic relationships of Glaucium Mill. in Turkey. Bangladesh Journal of Plant Taxonomy, 26(2), 259-268.
  21. Mobayen, S. (1985). Glaucium. In: Flora of Iran, vascular plants 3: 154 –170. Tehran University, Iran.
  22. Mory, B. (1979). Beitragezur Kenntnis der Sippenstruktur der Gattung Glaucium Miller (Papaveraceae). Feddes Repertorium 39: 499–595.
  23. Meirmans, P.G & Van Tienderen, P.H. (2004). GENOTYPE and GENODIVE: two programs for the analysis of genetic diversity of asexual organisms. Mol. Ecol. Notes. 4:792−794.
  24. Peakall, R. & Smouse, P.E. (2006) GENALEX 6: genetic analysis in Excel. Population genetic software for teaching and research. Molecular Ecology Notes 6: 288–295. https://doi.org/10.1111/j.1471-8286.2005.01155.x
  25. Podani, J. (2000) Introduction to the Exploration of Multivariate Data English translation. Backhuyes Publisher, Leide, 407 pp.
  26. Pritchard, J.K., Stephens, M. & Donnelly, P. (2000) Inference of population structure using multilocus genotype Data. Genetics 155: 945–959.
  27. Tavakkoli, Z. (2016). Notes on some species of the genus Glaucium (Papaveraceae) in Iran. Nova Biological Reperta 3: 167–176.
  28. Vafadar, M., Attar, F., Maroofi, H., (2010). Trichome micromorphology in drupe of Amygdalus L. (Rosaceae) from Iran. Acta Botanica Croatica 69: 93–105