10.57647/jnsc.2026.1604.20

A Eu-MOF Nanoarchitectured Electrochemiluminescent Sandwich Immunosensor for ProGRP Quantification in Serum and Pleural Effusion

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  • Linlin Luo1,
  • Chao Xu2,
  • Sicong Jiang1,
  • Yuxuan Xing3,
  • Zhiqian Zhao4,
  • Shuo Li*5,
  1. The Second Department of Respiratory Disease, Jiangxi Provincial People's Hospital (The First Affiliated Hospital of Nanchang Medical College), Nanchang, 330006, Jiangxi Province, China
  2. Department of Orthopedics, Shangrao Municipal Hospital (Affiliated Shangrao Municipal Hospital of Nanchang Medical College), Shangrao, 334000, China
  3. Department of Thoracic Surgery, The First Affiliated Hospital of Soochow University, Suzhou, 215006, China
  4. Department of Thoracic Surgery, The First Affiliated Hospital of Zhengzhou University, Zhengzhou University, Zhengzhou, 450000, China
  5. Department of Respiratory Medicine, The First Affiliated Hospital of Shandong First Medical University & Shandong Provincial Qianfoshan Hospital, Shandong Institute of Respiratory Diseases, Featured Laboratory of Respiratory Immunology and Regenerative Medicine in Universities of Shandong, Jinan Clinical Research Center for Respiratory Disease, Jinan 250014, China

Received: 22-02-2026

Revised: 04-04-2026

Accepted: 01-05-2026

Published in Issue 31-08-2026

Copyright (c) 2026 Linlin Luo, Chao Xu, Sicong Jiang, Yuxuan Xing, Zhiqian Zhao, Shuo Li (Author)

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

How to Cite

Luo, L., Xu, C., Jiang, S., Xing, Y., Zhao, Z., & Li, S. (2026). A Eu-MOF Nanoarchitectured Electrochemiluminescent Sandwich Immunosensor for ProGRP Quantification in Serum and Pleural Effusion. Journal of Nanostructure in Chemistry, 16(4 (August 2026). https://doi.org/10.57647/jnsc.2026.1604.20
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Abstract

Effective clinical management of small cell lung cancer (SCLC) requires sensitive and matrix-tolerant quantification of pro-gastrin-releasing peptide (ProGRP). Herein, we report a nanoarchitectured electrochemiluminescence (ECL) sandwich immunosensor that combines a three-dimensional reduced graphene oxide/gold (3D-rGO@Au) capture interface with a luminescent europium metal-organic framework (Eu-MOF) signal probe labeled with Ab2 for ProGRP measurement in serum and pleural effusion. The Eu-MOF formed uniform octahedral particles and provided efficient antenna-effect-assisted ECL emission with K₂S₂O₈ as the coreactant, while the 3D-rGO@Au interface increased antibody loading and accelerated interfacial electron transfer. The assay achieved a detection limit of 0.035 pg mL⁻¹ over a broad linear range, showed satisfactory recovery in two clinical matrices, and agreed well with commercial ECLIA measurements. Beyond combining two previously reported material families, the main contribution of this work is the clinically oriented integration of a high-loading conductive interface, a Eu-MOF luminophore probe, mechanistic ECL/CV/EIS validation, and real-sample verification for ProGRP detection.

Keywords

  • Clinical correlation,
  • 3D-rGO@Au interface,
  • Persulfate coreactant,
  • Small cell lung cancer,
  • Spike-and-recovery
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References

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