10.57647/jnsc.2025.1501.01

Core-shell nanoparticles based on ZnO/yeast cell wall: characterization, antifungal properties and aflatoxin B1 binding

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  • Ehsanolah Fakhar1,
  • Mansour Bayat1,
  • Anna Abdolshahi2,
  • Nakisa Sohrabi Haghdoost1,
  • Hamid Madanchi3,
  1. Department of Veterinary Pathobiology, S.R.C., Islamic Azad University, Tehran, Iran
  2. Food Safety (salt) Research Center, Semnan University of Medical Sciences, Semnan, Iran
  3. Nervous System Stem Cells Research Center Semnan University of Medical Sciences, Semnan, Iran   AND   Department of Medical Biotechnology, School of Medicine, Semnan University of Medical Sciences, Semnan, Iran   AND   Drug Design and Bioinformatics Unit, Medical Biotechnology Department, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran
Core-shell nanoparticles based on ZnO/yeast cell wall: characterization, antifungal properties and aflatoxin B1 binding

Received: 06-01-2025

Revised: 10-02-2025

Accepted: 28-02-2025

Published in Issue 28-02-2025

Copyright (c) 2025 Ehsanolah Fakhar, Mansour Bayat, Anna Abdolshahi, Nakisa Sohrabi Haghdoost, Hamid Madanchi (Author)

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This work is licensed under a Creative Commons Attribution 4.0 International License.

How to Cite

Fakhar, E. ., Bayat, M. ., Abdolshahi, A. ., Sohrabi Haghdoost, N. ., & Madanchi, H. . (2025). Core-shell nanoparticles based on ZnO/yeast cell wall: characterization, antifungal properties and aflatoxin B1 binding. Journal of Nanostructure in Chemistry, 15(1 (February 2025). https://doi.org/10.57647/jnsc.2025.1501.01
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Abstract

A class of mycotoxins such as aflatoxins are harmful and significant concern for public health, food safety, and agricultural practices.  Recent challenges are involve reducing and the eliminating aflatoxins from contaminated food and feed using bio-based strategies. The  development of edible binders is a promising approach to mitigate aflatoxin as a health risk. This study aimed to develop and  characterize of core-shell nanoparticles using yeast cell wall extracted from Saccharomyces Cerevisiae as a shell surrounding ZnO  nanoparticle core (CW/ZnO NPs). The obtained CW/ZnO core-shell was characterized regarding physicochemical properties, antifungal  effect and aflatoxin B1 (AFB1) binding capacity. FTIR confirmed the chemical composition of the synthesized nanoparticles. The size  distribution and zeta potential of CW/ZnO NPs were in the acceptable range. Based on the microscopy techniques, a spherical shape  (diameters of 21.36–42.54 nm) and a rough surface were observed, indicating a cell wall assigned to the shell of the ZnO core. According to the determined MIC and MFC, significant antifungal activity was obtained against A. parasiticus (500 µg/mL) and C.  albicans (125 µg/mL). The CW/ZnO core-shell showed the highest AFB1 adsorption (55.4%) compared to the cell wall and ZnO samples  in PBS. This research introduces a bio-based nanoparticle with a core-shell structure as an AFB1 binder. Considering its  potential for reducing aflatoxin bioavailability could be recommended to apply in the food and feed industry. 

Keywords

  • Cell wall,
  • Core-shell,
  • Aflatoxin,
  • ZnO,
  • S. cerevisiae,
  • Binding,
  • Nanoparticle
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References

  1. Muñoz-Zavala C, Molina-Macedo A, Toledo FH, Telles-Mejía E, Cabrera-Soto L, Palacios-Rojas N. Combating aflatoxin contamination by combining biocontrol application and adapted maize germplasm in northeastern and southeastern Mexico. Biological Control. 204, 105727 (2025)
  2. Javed M, Cao W, Tang L, Keener KM. A Review of Decontamination of Aspergillus spp. and Aflatoxin Control for Grains and Nuts with Atmospheric Cold Plasma. Toxins (Basel). 17, 129 (2025)
  3. Lv J, Zhang H, Wang X, Luo J, Wang Y, Kong D, et al. Occurrence, transfer rule, and dietary risk assessment of aflatoxins in medicinal and edible seeds. Food Biosci. 63, 105830 (2025)
  4. Shabeer S, Asad S, Jamal A, Ali A. Aflatoxin contamination, its impact and management strategies: an updated review. Toxins. 14, 307 (2022)
  5. Jallow A, Xie H, Tang X, Qi Z, Li P. Worldwide aflatoxin contamination of agricultural products and foods: From occurrence to control. Compr Rev Food Sci Food Saf. 20, 2332-81 (2021)
  6. Yaghmaee F, Aldaghi M, Naeimi M, Abdolshahi A, Fani SR, Mohammadi-Moghadam M. Reaction of Maize Varieties to Aspergillus flavus and Aflatoxin Production. J Chem Health Risks. 11, (2021)
  7. Ebrahimi A, Emadi A, Arabameri M, Jayedi A, Abdolshahi A, Yancheshmeh BS, et al. The prevalence of aflatoxins in different nut samples: A global systematic review and probabilistic risk assessment. AIMS Agric & Food 7, 130-48 (2022)
  8. Mousazadeh R, Hesaraki S, Bayat M, Jahandideh A, Hashemi J. Observation of anti-cancer effects of aflatoxin B1 in breast tumor category 4T1 of mice by injection around the tumor. J Comp Pathol. 18, 3625-34 (2021)
  9. Awuchi CG, Amagwula IO, Priya P, Kumar R, Yezdani U, Khan MG. Aflatoxins in foods and feeds: A review on health implications, detection, and control. Bull Environ Pharmacol Life Sci. 9, 149-55 (2020)
  10. Cao W, Yu P, Yang K, Cao D. Aflatoxin B1: Metabolism, toxicology, and its involvement in oxidative stress and cancer development. Toxicol Mech Methods. 32, 395-419 (2022)
  11. Commission CA, Commission CA. Code of practice for the prevention and reduction of aflatoxin contamination in peanuts. CAC/RCP. 55, (2004)
  12. Sipos P, Peles F, Brassó DL, Béri B, Pusztahelyi T, Pócsi I, et al. Physical and chemical methods for reduction in aflatoxin content of feed and food. Toxins. 13, 204 (2021)
  13. Loi M, Logrieco AF, Pusztahelyi T, Leiter É, Hornok L, Pócsi I. Advanced mycotoxin control and decontamination techniques in view of an increased aflatoxin risk in Europe due to climate change. Front Microbiol. 13, 1085891 (2023)
  14. SarveAhrabi Y, Khoei SN, Bayat M. Investigating of the Inhibitory Effects of Some Alkaloid Compounds against Aflatoxin biosynthesis polyketide synthase by Molecular Docking Method. Arch Adv Biosci. 15, 1-10 (2024)
  15. Yenew C, Mekonen S, Ambelu A, Yeshiwas AG. Effective Innovative Technologies and One Health Strategies in Mitigating Aflatoxin Contamination in Peanut Oil: A Systematic Review and Meta‐Analysis. Food Sci Nutr. 13, e70062 (2025)
  16. Jamaledin R, Sartorius R, Di Natale C, Onesto V, Manco R, Mollo V, et al. PLGA microparticle formulations for tunable delivery of a nano-engineered filamentous bacteriophage-based vaccine: in vitro and in silico-supported approach. J Nanostructure Chem. 14, 307-22 (2024)
  17. Zarei R, Sabokbar A, Rahimian Zarif B, Bayat M, Haghnazari N. Efficient biosorption of Zn (II), Cd (II), and Pb (II) by Aspergillus brasiliensis in industrial wastewater coupled with electrochemical monitoring via sensor enhanced with modified silver nanoparticles. Environ Sci Pollut Res Int. 1-16 (2024)
  18. Ahmed SF, Mofijur M, Rafa N, Chowdhury AT, Chowdhury S, Nahrin M, et al. Green approaches in synthesising nanomaterials for environmental nanobioremediation: Technological advancements, applications, benefits and challenges. Environ Res. 204(Pt A), 111967 (2022)
  19. Sadegh H, Ali GA, Gupta VK, Makhlouf ASH, Shahryari-Ghoshekandi R, Nadagouda MN, et al. The role of nanomaterials as effective adsorbents and their applications in wastewater treatment. J Nanostruct Chem. 7, 1-14 (2017)
  20. Hieu NH, Duyen DTM, Thang TQ, Duy PHA, Lam HDN, Phat LN, et al. Fabrication of reduced graphene oxide-doped carbon aerogels from water hyacinth for removal of methylene blue in water and energy storage. J Nanostruct Chem. 14, 383-401 (2024)
  21. Perera KY, Hopkins M, Jaiswal AK, Jaiswal S. Nanoclays-containing bio-based packaging materials: Properties, applications, safety, and regulatory issues. J Nanostruct Chem. 14, 71-93 (2024)
  22. Maleki A, Bochani S, Kermanian M, Makvandi P, Hosseini M-J, Hamidi M, et al. Chitosan conjugated-ordered mesoporous silica: A biocompatible dissolution enhancer for promoting the antidiabetic effect of a poorly water-soluble drug of repaglinide. J Nanostruct Chem. 14, 261-80 (2024)
  23. Karami-Osboo R, Hasantabar V, Maham M. Effective magnetic nanoadsorbent based on natural carboxymethyl cellulose/polyaniline nanotube/graphene oxide for aflatoxin B1 and B2 adsorption in rice utilizing a novel synthesis method. Int J Biol Macromol. 282(Pt 3), 137162 (2024)
  24. Jafarzadeh S, Nooshkam M, Zargar M, Garavand F, Ghosh S, Hadidi M, et al. Green synthesis of nanomaterials for smart biopolymer packaging: challenges and outlooks. J Nanostruct Chem. 14, 113-36 (2024)
  25. Ahmadian R, Larypoor M, Bayat M. Fabrication and Evaluation of PVA-NYS-THY Nanofiber Scaffolds as Antifungal Agents Against Fluconazole-Resistant Candida glabrata. Archives of Razi Institute. (2025)
  26. Khosravani N, Ahmadi V, Kakanejadifard A, Adeli M. Thermoresponsive and antibacterial two-dimensional polyglycerol-interlocked-polynipam for targeted drug delivery. J Nanostructure Chem. 14, 245-55 (2024)
  27. Ghosh Chaudhuri R, Paria S. Core/shell nanoparticles: classes, properties, synthesis mechanisms, characterization, and applications. Chem Rev. 112, 2373-433 (2012)
  28. Kumar R, Mondal K, Panda PK, Kaushik A, Abolhassani R, Ahuja R, et al. Core–shell nanostructures: perspectives towards drug delivery applications. J Mater Chem B. 8, 8992-9027 (2020)
  29. Banerjee M, Sharma S, Chattopadhyay A, Ghosh SS. Enhanced antibacterial activity of bimetallic gold-silver core–shell nanoparticles at low silver concentration. Nanoscale. 3, 5120-5 (2011)
  30. Hamza Z, El-Hashash M, Aly S, Hathout A, Soto E, Sabry B, et al. Preparation and characterization of yeast cell wall beta-glucan encapsulated humic acid nanoparticles as an enhanced aflatoxin B1 binder. Carbohydr Polym. 203, 185-92 (2019)
  31. Mirzaee Rad F, Tafvizi F, Noorbazargan H, Iranbakhsh A. Ag-doped ZnO nanoparticles synthesized through green method using Artemisia turcomanica extract induce cytotoxicity and apoptotic activities against AGS cancer cells: an in vitro study. J Nanostruct Chem. 14, 403-18 (2024)
  32. Gudkov SV, Burmistrov DE, Serov DA, Rebezov MB, Semenova AA, Lisitsyn AB. A mini review of antibacterial properties of ZnO nanoparticles. Front Phys. 9, 641481 (2021)
  33. Islam F, Shohag S, Uddin MJ, Islam MR, Nafady MH, Akter A, et al. Exploring the journey of zinc oxide nanoparticles (ZnO-NPs) toward biomedical applications. Materials (Basel). 15, 2160 (2022)
  34. Yammine M, Bray F, Flament S, Picavet A, Lacroix J-M, Poilpré E, et al. Reliable approach for pure yeast cell wall protein isolation from Saccharomyces cerevisiae yeast cells. ACS Omega. 7, 29702-13 (2022)
  35. Mohammadzade H, Hashemi‐Moghaddam H, Beikzadeh L, Ahmadieh-Yazdi A, Madanchi H, Fallah P. Molecular imprinting of miR-559 on a peptide-immobilized poly L-DOPA/silica core–shell and in vitro investigating its effects on HER2-positive breast cancer cells. Drug Deliv Transl Res. 13, 2487-502 (2023)
  36. AbdElhady M. Preparation and characterization of chitosan/zinc oxide nanoparticles for imparting antimicrobial and UV protection to cotton fabric. Int J Carbohydr Chem. 2012, 840591 (2012)
  37. Nikookar Golestani R, Ghods E, Rostamian M, Madanchi H, Talebi AF. Investigating the antimicrobial activity, cytotoxicity, and action mechanism of acylated and amidated derivatives of AurH1 antifungal peptide. BMC Microbiol. 23, 332 (2023)
  38. Manzar R, Mahmood S, Farrukh MA, Laraib Q, Nawaz R, Qamar MT, et al. Graphene oxide-mediated green synthesis of Ag, Co3O4 and ZnO nanocomposites for multifunctional antimicrobial applications. J Solgel Sci Technol. 113, 573-91 (2025)
  39. Ma W, Zhu G, Zhang Y, Guo J. Green synthesis of ZnO NPs with long-lasting and ultra-high antimicrobial activity. Surf Interfaces. 50, 104506 (2024)
  40. Vahidimehr A, Khiabani MS, Mokarram RR, Kafil HS, Ghiasifar S, Vahidimehr A. Saccharomyces cerevisiae and Lactobacillus rhamnosus cell walls immobilized on nano-silica entrapped in alginate as aflatoxin M1 binders. Int J Biol Macromol. 164, 1080-6 (2020)
  41. Dhanalekshmi K, Meena K. Comparison of antibacterial activities of Ag@ TiO2 and Ag@ SiO2 core–shell nanoparticles. Spectrochim Acta A Mol Biomol Spectrosc. 128, 887-90 (2014)
  42. Sharaf EM, Hassan A, Al-Salmi FA, Albalwe FM, Albalawi HMR, Darwish DB, et al. Synergistic antibacterial activity of compact silver/magnetite core-shell nanoparticles core shell against Gram-negative foodborne pathogens. Front Microbiol. 13, 929491 (2022)
  43. Elattar KM, Al-Otibi FO, El-Hersh MS, Attia AA, Eldadamony NM, Elsayed A, et al. Multifaceted chemical and bioactive features of Ag@ TiO2 and Ag@ SeO2 core/shell nanoparticles biosynthesized using Beta vulgaris L. extract. Heliyon. 10, e28359 (2024)