10.1186/2193-8865-3-7

A study on the assessment of DNA strand-breaking activity by silver and silica nanoparticles

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  • Ali R Vahdati1,
  • Babak Sadeghi*2,
  1. Young Researchers Club, Tonekabon Branch, Islamic Azad University, Tonekabon, IR
  2. Department of Chemistry, Tonekabon Branch, Islamic Azad University Tonekabon, Tonekabon, IR
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Published in Issue 27-03-2013

How to Cite

Vahdati, A. R., & Sadeghi, B. (2013). A study on the assessment of DNA strand-breaking activity by silver and silica nanoparticles. Journal of Nanostructure in Chemistry, 3(1 (December 2013). https://doi.org/10.1186/2193-8865-3-7
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Abstract

Abstract We measured the DNA strand-breaking activity of nanoparticle by agarose gel electrophoresis. Plasmid DNA was mixed with nanosilver and nano-silica powders in an aqueous solution. Then the mixture was applied to 1% of agarose gel electrophoresis at 75 V for 30 min. We used 300 nm of UV irradiation in order to visualize the DNA bands. The DNA strand-breaking activity of nanomaterial was measured through measuring the reduction of super-coiled DNA to circular form of DNA.

Keywords

  • Silver nanoparticles,
  • DNA,
  • Nanotechnology
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