10.1007/s40097-014-0112-3

Biosynthesis of silver nanoparticles using extract of olive leaf: synthesis and in vitro cytotoxic effect on MCF-7 cells

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  • Marzieh Rashidipour1,
  • Rouhollah Heydari*2,
  1. Young Researchers and Elite Club, Khorram Abad Branch, Islamic Azad University, Khorramabad, IR
  2. Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, IR
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Published in Issue 11-06-2014

How to Cite

Rashidipour, M., & Heydari, R. (2014). Biosynthesis of silver nanoparticles using extract of olive leaf: synthesis and in vitro cytotoxic effect on MCF-7 cells. Journal of Nanostructure in Chemistry, 4(3 (September 2014). https://doi.org/10.1007/s40097-014-0112-3
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Abstract

Abstract A green synthetic approach using olive leaf extract was optimized for preparing silver nanoparticles (AgNPs). Parameters affecting the formation of nanoparticles such as temperature, exposure time to extract, pH, concentration of silver nitrate, and extract (ratio of plant sample to extraction solvent) were investigated and optimized. Optimum conditions for the synthesis of silver nanoparticles are as follows: Ag + concentration 1 mM, extract concentration, 8 % w/v; pH = 7, time, 4 h and temperature, 45 °C. Synthesized silver nanoparticles were characterized using UV–Visible absorption spectroscopy, Fourier-transform infrared spectroscopy, X-ray diffraction, dynamic light scattering (DLS), and scanning electron microscopy (SEM). SEM and DLS analysis showed that the synthesized AgNPs were predominantly spherical in shape with an average size of 90 nm. The cytotoxicity activities of the synthesized AgNPs and olive leaves extract containing AgNPs against human breast cancer cell (MCF-7) were investigated and the inhibitory concentration (IC 50 ) was found to be 50 and 0.024 μg/mL at 24 h incubation, respectively. This eco-friendly and cost-effective synthesis method can be potentially used for large-scale production of silver nanoparticles.

Keywords

  • Silver nanoparticles,
  • Olive leaf extract,
  • Anticancer,
  • MCF-7
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References

  1. Hu et al. (2009) Metallic nanostructures as localized plasmon resonance enhanced scattering probes for multiplex dark field targeted imaging of cancer cells (pp. 2676-2684) https://doi.org/10.1021/jp8076672
  2. Yin et al. (2003) Electrochemical synthesis of silver nanoparticles under protection of poly(N-vinylpyrrolidone) (pp. 8898-8904) https://doi.org/10.1021/jp0349031
  3. Dimitrijevic et al. (2001) Radiolytically induced formation and optical absorption spectra of colloidal silver nanoparticles in supercritical ethane (pp. 954-959) https://doi.org/10.1021/jp0028296
  4. Wang et al. (2013) Ionic-liquid-assisted facile synthesis of silver nanoparticle-reduced graphene oxide hybrids by gamma irradiation (pp. 245-252) https://doi.org/10.1016/j.carbon.2012.12.033
  5. Callegari et al. (2003) Photochemically grown silver nanoparticles with wavelength-controlled size and shape (pp. 1565-1568) https://doi.org/10.1021/nl034757a
  6. Pandey et al. (2012) Green synthesis of biopolymer–silver nanoparticle nanocomposite: an optical sensor for ammonia detection (pp. 583-589) https://doi.org/10.1016/j.ijbiomac.2012.06.033
  7. Gopinathan et al. (2013) Bacterial flagella as biotemplate for the synthesis of silver nanoparticle impregnated bionanomaterial (pp. 717-722) https://doi.org/10.1016/j.apsusc.2013.03.159
  8. Marimuthu et al. (2011) Evaluation of green synthesized silver nanoparticles against parasites (pp. 1541-1549) https://doi.org/10.1007/s00436-010-2212-4
  9. Nayak et al. (2011) Green synthesis of silver nanoparticle by Penicillium purpurogenum NPMF: the process and optimization (pp. 3129-3137) https://doi.org/10.1007/s11051-010-0208-8
  10. Sivaraman et al. (2009) A green protocol for room temperature synthesis of silver nanoparticles in seconds (pp. 1055-1059)
  11. Martínez-Castañón et al. (2008) Synthesis and antibacterial activity of silver nanoparticles with different sizes (pp. 1343-1348) https://doi.org/10.1007/s11051-008-9428-6
  12. Lok et al. (2007) Silver nanoparticles: partial oxidation and antibacterial activities (pp. 527-534) https://doi.org/10.1007/s00775-007-0208-z
  13. Sondi and Salopek-Sondi (2004) Silver nanoparticles as antimicrobial agent: a case study on E. coli as a model for Gram-negative bacteria (pp. 177-182) https://doi.org/10.1016/j.jcis.2004.02.012
  14. Sukirtha et al. (2012) Cytotoxic effect of Green synthesized silver nanoparticles using Melia azedarach against in vitro HeLa cell lines and lymphoma mice model (pp. 273-279) https://doi.org/10.1016/j.procbio.2011.11.003
  15. Vivek et al. (2012) Green biosynthesis of silver nanoparticles from Annona squamosa leaf extract and its in vitro cytotoxic effect on MCF-7 cells (pp. 2405-2410) https://doi.org/10.1016/j.procbio.2012.09.025
  16. Miura and Shinohara (2009) Cytotoxic effect and apoptosis induction by silver nanoparticles in HeLa cells (pp. 733-737) https://doi.org/10.1016/j.bbrc.2009.10.039
  17. Hsin et al. (2008) The apoptotic effect of nanosilver is mediated by a ROS- and JNK-dependent mechanism involving the mitochondrial pathway in NIH3T3 cells (pp. 130-139) https://doi.org/10.1016/j.toxlet.2008.04.015
  18. Asha Rani et al. (2009) Cytotoxicity and genotoxicity of silver nanoparticles in human cells (pp. 279-290) https://doi.org/10.1021/nn800596w
  19. Bernal et al. (2010) Advanced analysis of nutraceuticals (pp. 758-774) https://doi.org/10.1016/j.jpba.2010.11.033
  20. Owen et al. (2000) The antioxidant/anticancer potential of phenolic compounds isolated from olive oil (pp. 1235-1247) https://doi.org/10.1016/S0959-8049(00)00103-9
  21. Tripoli et al. (2005) The phenolic compounds of olive oil: structure, biological activity and beneficial effects on human health (pp. 98-112) https://doi.org/10.1079/NRR200495
  22. Reboredo-Rodríguez et al. (2014) Ultrasound-assisted emulsification–microextraction for the determination of phenolic compounds in olive oils (pp. 128-136) https://doi.org/10.1016/j.foodchem.2013.10.157
  23. Hayes et al. (2011) Phenolic composition and in vitro antioxidant capacity of four commercial phytochemical products: olive leaf extract (Olea europaea L.), lutein, sesamol and ellagic acid (pp. 948-955) https://doi.org/10.1016/j.foodchem.2010.11.092
  24. Denizot and Lang (1986) Rapid colorimetric assay for cell growth and survival. Modifications to the tetrazolium dye procedure giving improved sensitivity and reliability (pp. 271-277) https://doi.org/10.1016/0022-1759(86)90368-6