10.1007/s40097-018-0273-6

Preparation and characterization of ascorbic acid-mediated chitosan–copper oxide nanocomposite for anti-microbial, sporicidal and biofilm-inhibitory activity

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  • S. Logpriya1,
  • V. Bhuvaneshwari*1,
  • D. Vaidehi1,
  • R. P. SenthilKumar1,
  • R. S. Nithya Malar1,
  • B. Pavithra Sheetal1,
  • R. Amsaveni1,
  • M. Kalaiselvi2,
  1. PG and Research Department of Biotechnology, Kongunadu Arts and Science College, Coimbatore, Tamilnadu, 029, IN
  2. Department of Biochemistry, Kongunadu Arts and Science College, Coimbatore, Tamilnadu, 029, IN
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Published in Issue 14-08-2018

How to Cite

Logpriya, S., Bhuvaneshwari, V., Vaidehi, D., SenthilKumar, R. P., Nithya Malar, R. S., Pavithra Sheetal, B., Amsaveni, R., & Kalaiselvi, M. (2018). Preparation and characterization of ascorbic acid-mediated chitosan–copper oxide nanocomposite for anti-microbial, sporicidal and biofilm-inhibitory activity. Journal of Nanostructure in Chemistry, 8(3 (September 2018). https://doi.org/10.1007/s40097-018-0273-6
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Abstract

Abstract The chitosan–copper oxide (Chi–CuO) biopolymer nanocomposites were synthesized by a simple green chemistry method using ascorbic acid as a reducing and capping agent. The intense peak around 300 nm was observed in the UV–visible spectrum indicating the formation of CuO nanoparticles. The prepared Chi–CuO nanocomposites were characterized using energy-dispersive X-ray spectroscopy (EDX), scanning electron microscopy (SEM), X-ray diffraction spectroscopy (XRD), and Fourier transform-infrared spectroscopy (FT-IR). SEM and XRD pattern showed cubic shape for Chi–CuO nanocomposites with average crystalline size of 17 nm, as calculated using Debye–Scherrer’s formula. The FT-IR spectral studies showed the Cu–O bond formation with chitosan to form nanocomposites. Synthesized nanocomposites showed significant anti-microbial activity against Bacillus subtilis , Pseudomonas aeruginosa , Escherichia coli and Penicillium notatum , assayed using the agar well diffusion method. It also showed sporicidal activity against B. subtilis and exhibited effective biofilm-inhibitory activity against B. subtilis (69%/100 μg/mL) and P. aeruginosa (63%/100 μg/mL). Graphical abstract

Keywords

  • Biopolymer nanocomposites,
  • Chitosan,
  • Copper oxide,
  • Anti-microbial activity,
  • Biofilm inhibition activity
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