10.1007/s40097-016-0205-2

Antibiofilm activity of biogenic copper and zinc oxide nanoparticles-antimicrobials collegiate against multiple drug resistant bacteria: a nanoscale approach

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  • C. Ashajyothi1,
  • K. Handral Harish2,
  • Nileshkumar Dubey2,
  • R. Kelmani Chandrakanth*1,
  1. Department of Biotechnology, Gulbarga University, Gulbarga, Karnataka, 585106, IN
  2. Oral Sciences Disciplines, Faculty of Dentistry, National University of Singapore, Singapore, 117510, SG
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Published in Issue 30-09-2016

How to Cite

Ashajyothi, C., Harish, K. H., Dubey, N., & Chandrakanth, R. K. (2016). Antibiofilm activity of biogenic copper and zinc oxide nanoparticles-antimicrobials collegiate against multiple drug resistant bacteria: a nanoscale approach. Journal of Nanostructure in Chemistry, 6(4 (December 2016). https://doi.org/10.1007/s40097-016-0205-2
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Abstract

Abstract The synthesis of biogenic nanoparticles from non-chemical resources has increased the drive toward understanding infection biology. Accordingly, we aimed to address the symbiotic antibiofilm effect of biogenic copper and zinc oxide nanoparticles with antimicrobials against multidrug resistant (MDR) pathogens. The minimum inhibitory concentration (MIC) of copper nanoparticles (CuNPs) and zinc oxide nanoparticles (ZnONPs) at the range from 2 to 128 µg/ml was calculated against Gram-positive and Gram-negative pathogenic bacteria using a broth dilution method. Both nanoparticles have prime antibacterial activity compared with standard antibiotics (excluding against P.aeruginosa MTCC 741). A qualitative assessment of biofilm formation and collegial effect was performed using a modified test tube and the microtiter plate-based method by measuring the optical density and time kill of nanoparticles. The results demonstrated efficient antibiofilm activity of CuNPs in its lowest concentration than ZnONPs and antibiotics itself. In addition, significant enhancing antibiofilm effect was also shown by CuNPs in the presence of third generation antibiotics against Gram-negative and Gram-positive bacteria. A scanning electron microscopy (SEM) analysis was used to investigate the effect of the nanoparticles on morphological changes of Staphylococcus aureus . Current data highlights, biogenic CuNPs and ZnONPs could be used as an adjuvant for antibiotics in the treatment of bacterial infections.

Keywords

  • Biogenic nanoparticles,
  • Test tube method,
  • Microtiter plate method,
  • Scanning electron microscopy,
  • Antibiofilm activity
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