10.1007/s40097-015-0148-z

Fabrication of DNA nanotubes using origami-based nanostructures with sticky ends

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  • Mohammad Mousavi-Khattat1,
  • Adele Rafati1,
  • Pooria Gill*2,
  1. Department of Medical Biotechnology, Faculty of Advanced Medical Technologies, Golestan University of Medical Sciences, Gorgan, IR
  2. Department of Medical Biotechnology, Faculty of Advanced Medical Technologies, Golestan University of Medical Sciences, Gorgan, IR Nanomedicine Group, Research Center for Immunogenetics, Mazandaran University of Medical Sciences, Sari, IR Department of Physio Pharmacology and NanoBioMedicine, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, IR
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Published in Issue 05-02-2015

How to Cite

Mousavi-Khattat, M., Rafati, A., & Gill, P. (2015). Fabrication of DNA nanotubes using origami-based nanostructures with sticky ends. Journal of Nanostructure in Chemistry, 5(2 (June 2015). https://doi.org/10.1007/s40097-015-0148-z
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Abstract

Abstract Described here is a simplified method for fabrication of DNA nanotubes using a minimum numbers of staple oligomers for DNA origami. For this purpose, the cylindrical nanotemplates with two sticky ends have been designed using caDNAno software. Then, the nanostructures were shaped in an optimized experimental condition via an origami-based self-assembly reaction. Finally, the produced nanostructures were joined together through their sticky ends using a ligase enzyme. Transmission electron microscope confirmed fabrication of these elongated nanotubes. In addition, high-resolution microscopy of DNA nanotubes by scanning tunnelling microscope indicated efficient attachments of the primarily DNA nanostructures via their sticky ends. The results demonstrated that a ligase treatment of cylindrical DNA nanostructures with the sticky ends made DNA nanotubes with standard shapes using minimum numbers of staples.

Keywords

  • DNA nanotube,
  • T4 ligase,
  • Sticky ends,
  • TEM,
  • STM
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