10.1007/s40097-014-0125-y

In situ silver nanoparticle formation embedded into a photopolymerized hydrogel with biocide properties

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  • Carmen Mabel González Henríquez*1,
  • Guadalupe del Carmen Pizarro Guerra1,
  • Mauricio Alejandro Sarabia Vallejos2,
  • Susana Dennis Rojas de la Fuente2,
  • María Teresa Ulloa Flores3,
  • Lina María Rivas Jimenez3,
  1. Chemistry Department, Metropolitan Technological University, Santiago, 7800002, CL
  2. Physics Institute, Pontifical Catholic University of Chile, Santiago, 7820436, CL
  3. Microbioloy and Mycology Program, ICBM, Medicine Faculty, University of Chile, Santiago, 8380453, CL
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Published in Issue 18-09-2014

How to Cite

Henríquez, C. M. G., del Carmen Pizarro Guerra, G., Vallejos, M. A. S., de la Fuente, S. D. R., Flores, M. T. U., & Jimenez, L. M. R. (2014). In situ silver nanoparticle formation embedded into a photopolymerized hydrogel with biocide properties. Journal of Nanostructure in Chemistry, 4(4 (December 2015). https://doi.org/10.1007/s40097-014-0125-y
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Abstract

Abstract In situ nanoparticle formation embedded into hydrogel matrix, acting as container and stabilizer for nanoparticle reaction was the focus in this research; this method was realized using AgNO 3 (0.75 and 1.0 M) as silver source for nanoparticle formation; also, monomers (HEMA), cross-linker agents (DEGDMA) and a photoinitiator (Irgacure 651) were used for the hydrogel synthesis. For the reduction of Ag +  → Ag 0 , the reaction mixture was irradiated with an UV lamp at 365 nm for 30 min; parallel to this process, the hydrogel photopolymerization occurs. All these systems were studied by Infrared and Raman Spectroscopy, optical studies: UV/Vis absorption, thermal studies: differential scanning calorimetry and thermogravimetric analysis, X-ray diffraction, fluorescence X-ray spectroscopy and transmission electronic microscopy. Characterization techniques are capable to detect the presence of non-agglomerated silver nanoparticles homogeneously distributed in all the systems. X-Ray photoemission spectroscopy establishes the presence of Ag 0 and Ag + as mixture in the synthesized composite. Quantitative assays show that the sample Ag_Hg3-89.5 % (1.0 M) presents an important biocide property, by reducing 99.9 % of bacterium Escherichia coli ATCC 25922 as compared with the alone hydrogel used as control. GRAPHICAL ABSTRACT The embedding of silver nanoparticles homogenously distributed and stabilized into the hydrogel matrix was realized in situ using a photoinitiator during the synthesis. In figures it is possible to observe different conformations of hydrogel/silver nanoparticle composites, so the non-organic nanoparticles seem to be confined in the organic polymer matrix, forming a bunch. TEM analysis shows spherical nanoparticles with diameters around 22–37 nm. Biocide tests show that this compound could be utilized as E. coli bactericide with remarkable results.

Keywords

  • Photopolymerizable hydrogel,
  • Crosslinking agent,
  • Silver nanoparticles,
  • Biocide properties,
  • E. Coli
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References

  1. Scognamillo et al. (2010) Thermoresponsive super water absorbent hydrogels prepared by frontal polymerization 48(1) (pp. 2486-2490) https://doi.org/10.1002/pola.24020
  2. Hoffman (2012) Hydrogels for biomedical applications (pp. 18-23) https://doi.org/10.1016/j.addr.2012.09.010
  3. Yu et al. (2010) Biodegradability and biocompatibility of thermoreversible hydrogels formed from mixing a sol and a precipitate of block copolymer in water 11(8) (pp. 2169-2178) https://doi.org/10.1021/bm100549q
  4. Qiu and Park (2012) Environment-sensitive hydrogels for drug delivery (pp. 49-60) https://doi.org/10.1016/j.addr.2012.09.024
  5. Wichterle and Lim (1960) Hydrophilic gels for biological use (pp. 117-118) https://doi.org/10.1038/185117a0
  6. Hoffman et al. (1972) Covalent binding of biomolecules to radiation-grafted hydrogels on inert polymer surfaces (pp. 10-17) https://doi.org/10.1097/00002480-197201000-00003
  7. Ratner and Hoffman (1976) Synthetic hydrogels for biomedical applications, in hydrogels for medical and related applications (pp. 1-36) https://doi.org/10.1021/bk-1976-0031.ch001
  8. Peppas (1987) (pp. 95-126) CRC Press
  9. Park, K., Shalaby, W.S.W., Park, H.: Biodegradable Hydrogels for Drug Delivery. Technomic, Lancaster, PA, ch. 9, pp. 233–238 (1993)
  10. Harland, R.S., Prud’homme, R.K.: Polyelectrolyte Gels: Properties, Preparation and Applications, American Chemical Society, Washington, DC, vol. 480, ch. 16, pp. 269–284 (1992)
  11. Ulbrich et al. (1995) Synthesis of novel hydrolytically degradable hydrogels for controlled drug (pp. 155-165) https://doi.org/10.1016/0168-3659(95)00004-R
  12. Hoffman (1995) Intelligent polymers in medicine and biotechnology (pp. 645-664) https://doi.org/10.1002/masy.19950980156
  13. Harris and Zalipsky (1997) ACS Sym. Ser https://doi.org/10.1021/bk-1997-0680
  14. Su et al. (2010) Anti-inflammatory peptide-functionalized hydrogels for insulin-secreting cell encapsulation 31(2) (pp. 308-314) https://doi.org/10.1016/j.biomaterials.2009.09.045
  15. Kumachev et al. (2011) High-throughput generation of hydrogel microbeads with varying elasticity for cell encapsulation 32(6) (pp. 1477-1483) https://doi.org/10.1016/j.biomaterials.2010.10.033
  16. Phelps et al. (2012) Maleimide cross-linked bioactive PEG hydrogel exhibits improved reaction kinetics and cross-linking for cell encapsulation and in situ delivery 24(1) (pp. 64-70) https://doi.org/10.1002/adma.201103574
  17. Liu et al. (2012) Generation of disk-like hydrogel beads for cell encapsulation and manipulation using a droplet-based microfluidic device 13(5) (pp. 761-767) https://doi.org/10.1007/s10404-012-0998-3
  18. Zhu (2010) Bioactive modification of poly(ethylene glycol) hydrogels for tissue engineering 31(17) (pp. 4639-4656) https://doi.org/10.1016/j.biomaterials.2010.02.044
  19. Van Vlierberghe et al. (2011) Biopolymer-based hydrogels as scaffolds for tissue engineering applications: a review 12(5) (pp. 1387-1408) https://doi.org/10.1021/bm200083n
  20. Tan et al. (2009) Injectable in situ forming biodegradable chitosan-hyaluronic acid based hydrogels for cartilage tissue engineering 30(13) (pp. 2499-2506) https://doi.org/10.1016/j.biomaterials.2008.12.080
  21. Zhu and Marchant (2011) Design properties of hydrogel tissue-engineering scaffolds 8(5) (pp. 607-626) https://doi.org/10.1586/erd.11.27
  22. Kostina et al. (2013) Novel antifouling self-healing poly(carboxybetaine methacrylamide-co-HEMA) nanocomposite hydrogels with superior mechanical properties (pp. 5644-5650) https://doi.org/10.1039/c3tb20704h
  23. Gawel et al. (2012) Impregnation of weakly charged anionic microhydrogels with cationic polyelectrolytes and their swelling properties monitored by a high resolution interferometric technique. Transformation from a polyelectrolyte to polyampholyte hydrogel 48(11) (pp. 1949-1959) https://doi.org/10.1016/j.eurpolymj.2012.07.006
  24. Vilozny et al. (2011) Multiwell plates loaded with fluorescent hydrogel sensors for measuring pH and glucose concentration (pp. 7589-7595) https://doi.org/10.1039/c0jm04257a
  25. Kali et al. (2013) Thermally responsive amphiphilic conetworks and gels based on poly(N-isopropylacrylamide) and polyisobutylene 46(13) (pp. 5337-5344) https://doi.org/10.1021/ma400535r
  26. Huang et al. (2014) Conducting hydrogels of tetraaniline-g-poly(vinyl alcohol) in situ reinforced by supramolecular nanofibers 6(3) (pp. 1595-1600) https://doi.org/10.1021/am4043799
  27. Pafiti et al. (2014) “Inverse polyampholyte” hydrogels from double-cationic hydrogels: synthesis by RAFT polymerization and characterization 47(5) (pp. 1819-1827) https://doi.org/10.1021/ma500084c
  28. Way et al. (2014) Enhancing the mechanical properties of guanosine-based supramolecular hydrogels with guanosine-containing polymers 47(5) (pp. 1810-1818) https://doi.org/10.1021/ma402618z
  29. Qingzhou et al. (2012) A Combined physical-chemical polymerization process for fabrication of nanoparticle-hydrogel sensing materials 45(20) (pp. 8382-8386) https://doi.org/10.1021/ma301119f
  30. Tuncaboylu et al. (2012) Dynamics and large strain behavior of self-healing hydrogels with and without Surfactants 45(4) (pp. 1991-2000) https://doi.org/10.1021/ma202672y
  31. Yue et al. (2009) Fabrication and characterization of microstructured and pH sensitive interpenetrating networks hydrogel films and application in drug delivery field 45(2) (pp. 309-315) https://doi.org/10.1016/j.eurpolymj.2008.10.038
  32. Guiseppi-Eliea (2010) Electroconductive hydrogels: synthesis, characterization and biomedical applications 31(10) (pp. 2701-2716) https://doi.org/10.1016/j.biomaterials.2009.12.052
  33. Jagur-Grodzinski (2010) Polymeric gels and hydrogels for biomedical and pharmaceutical applications 21(1) (pp. 27-47)
  34. Baït et al. (2011) Hydrogel nanocomposites as pressure-sensitive adhesives for skin-contact applications (pp. 2025-2032) https://doi.org/10.1039/c0sm01123a
  35. Li and Lee (2005) Photopolymerization of HEMA/DEGDMA hydrogels in solution 46(25) (pp. 11540-11547) https://doi.org/10.1016/j.polymer.2005.10.051
  36. Otsuka et al. (2012) PEGylated nanoparticles for biological and pharmaceutical applications (pp. 246-255) https://doi.org/10.1016/j.addr.2012.09.022
  37. Petros and DeSimone (2010) Strategies in the design of nanoparticles for therapeutic applications 9(8) (pp. 615-627) https://doi.org/10.1038/nrd2591
  38. Sau et al. (2010) Properties and applications of colloidal nonspherical noble metal nanoparticles 22(16) (pp. 1805-1825) https://doi.org/10.1002/adma.200902557
  39. Hao et al. (2010) Synthesis, functionalization and biomedical applications of multifunctional magnetic nanoparticles 22(25) (pp. 2729-2742) https://doi.org/10.1002/adma.201000260
  40. Jain et al. (2009) Silver nanoparticles in therapeutics: development of an antimicrobial gel formulation for topical use 6(5) (pp. 1388-1401) https://doi.org/10.1021/mp900056g
  41. Verma et al. (2010) Biosynthesis of antimicrobial silver nanoparticles by the endophytic fungus Aspergillus clavatus 5(1) (pp. 33-40) https://doi.org/10.2217/nnm.09.77
  42. Singhal et al. (2011) Biosynthesis of silver nanoparticles using Ocimum sanctum (Tulsi) leaf extract and screening its antimicrobial activity 13(7) (pp. 2981-2988) https://doi.org/10.1007/s11051-010-0193-y
  43. Rai et al. (2009) Silver nanoparticles as a new generation of antimicrobials 27(1) (pp. 76-83) https://doi.org/10.1016/j.biotechadv.2008.09.002
  44. Sharma et al. (2009) Silver nanoparticles: green synthesis and their antimicrobial activities 145(1–2) (pp. 83-96) https://doi.org/10.1016/j.cis.2008.09.002
  45. Kim et al. (2011) Antibacterial activity of silver-nanoparticles against Staphylococcus aureus and Escherichia coli 39(1) (pp. 77-85)
  46. Varsha et al. (2011) In situ formation of silver nanoparticles within Chitosan-attached cotton fabric for antibacterial property 40(3) (pp. 229-245) https://doi.org/10.1177/1528083710371490
  47. Agnihotri et al. (2012) Antimicrobial chitosan–PVA hydrogel as a nanoreactor and immobilizing matrix for silver nanoparticles 2(3) (pp. 179-188) https://doi.org/10.1007/s13204-012-0080-1
  48. Vermonden et al. (2012) Hydrogels for protein delivery 112(5) (pp. 2853-2888) https://doi.org/10.1021/cr200157d
  49. Lin-Vien et al. (1991) Academic Press
  50. Cook et al. (2011) Simultaneous photoinduced silver nanoparticles formation and cationic polymerization of divinyl ethers 44(11) (pp. 4065-4071) https://doi.org/10.1021/ma200575n
  51. Guzman et al. (2012) Synthesis and antibacterial activity of silver nanoparticles against gram-positive and gram-negative bacteria (pp. 37-45) https://doi.org/10.1016/j.nano.2011.05.007
  52. Fendler and Dékány (1997) Springer
  53. Yao et al. (2010) Sliding-graft interpenetrating polymer networks from simultaneous “Click Chemistry” and atom transfer radical polymerization 43(23) (pp. 9761-9770) https://doi.org/10.1021/ma102039n
  54. Uygun et al. (2011) Photopolymerization kinetics and dynamic mechanical properties of silanes hydrolyzed without evolution of byproducts. Tetrakis[(methacryloyloxy)ethoxy]silane−Diethylene glycol dimethacrylate 44(7) (pp. 1792-1800) https://doi.org/10.1021/ma102903q
  55. Wornyo, E: Fabrication and Characterization of Shape Memory Polymers at Small-scales, ProQuest, p. 51. UMI Dissertation Publishing, Ann Harbor, Michigan, USA (2011)
  56. Huma et al. (2014) Crosslinking of poly(N-vinyl pyrrolidone-co-n-butyl methacrylate) copolymers for controlled drug delivery (pp. 433-445) https://doi.org/10.1007/s00289-013-1069-y
  57. Goiti et al. (2007) Effect of magnetic nanoparticles on the thermal properties of some hydrogels (pp. 2198-2205) https://doi.org/10.1016/j.polymdegradstab.2007.02.025
  58. Carrol et al. (2011) Preparation of elemental Cu and Ni nanoparticles by the polyol method: an experimental and theoretical approach 115(6) (pp. 2656-2664) https://doi.org/10.1021/jp1104196
  59. Prieto et al. (2012) XPS study of silver, nickel and bimetallic silver-nickel nanoparticles prepared by seed-mediated growth 258(22) (pp. 8807-8813) https://doi.org/10.1016/j.apsusc.2012.05.095
  60. Jeon and Kang (2008) Synthesis and characterization of indium-tin-oxide particles prepared using sol–gel and solvothermal methods and their conductivities after fixation on polyethyleneterephthalate films 62(4–5) (pp. 676-682) https://doi.org/10.1016/j.matlet.2007.06.038
  61. Ferraria et al. (2012) X-ray photoelectron spectroscopy: silver salts revisited 86(12) (pp. 1988-1991) https://doi.org/10.1016/j.vacuum.2012.05.031
  62. Sumesh et al. (2011) A practical silver nanoparticle-based adsorbent for the removal of Hg2+ from water 189(1–2) (pp. 450-457) https://doi.org/10.1016/j.jhazmat.2011.02.061
  63. Satta and Moretti (2010) Auger parameters and Wagner plots (pp. 123-127) https://doi.org/10.1016/j.elspec.2009.07.008