10.1007/s40097-022-00513-1

Protective effect of melanin nanoparticles created from squid ink against irradiation on human keratinocytes

  • Le Na Nguyen Thi*1,
  • Son Le Duc1,
  • Van Khanh Bui Thi1,
  • Thanh Tam Dinh Thi2,
  • Hai Do Xuan3,
  • My Nhung Hoang Thi4,
  • Thang Nguyen Dinh1,
  1. Department of Biochemistry and Molecular Biology, Faculty of Biology, VNU University of Science, Vietnam National University, Hanoi, VN
  2. Center of Applied Sciences, Regenerative Medicine and Advance Technologies (CARA)–Vinmec Healthcare System, Hanoi, VN
  3. Department of Practical and Experimental Surgery, Vietnam Military Medical University, Hanoi, VN
  4. Department of Biochemistry and Molecular Biology, Faculty of Biology, VNU University of Science, Vietnam National University, Hanoi, VN National Key Laboratory of Enzyme and Protein Technology, VNU University of Science, Vietnam National University, Hanoi, VN
Protective effect of melanin nanoparticles created from squid ink against irradiation on human keratinocytes

Published in Issue 20-09-2022

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This work is licensed under a Creative Commons Attribution 4.0 International License.

How to Cite

Nguyen Thi, L. N., Le Duc, S., Bui Thi, V. K., Dinh Thi, T. T., Do Xuan, H., Hoang Thi, M. N., & Nguyen Dinh, T. (2022). Protective effect of melanin nanoparticles created from squid ink against irradiation on human keratinocytes. Journal of Nanostructure in Chemistry, 14(3 (June 2024). https://doi.org/10.1007/s40097-022-00513-1
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Abstract

Abstract Because the skin is the first organ to be impacted by radiation, keratinocytes (HaCaT cells) are frequently used as an  in vitro model for testing potential radioprotective agents. Melanin extracted from squid ink was considered an antioxidant compound and a possible radioprotector. In this study, nanomelanin was prepared at a size of 100–200 nm from isolated squid melanin. The zeta potential of melanin nanoparticles and the functional groups on their surface were investigated. The antioxidant activity of nanomelanin was examined by the DPPH method. Keratinocyte cells were incubated with or without melanin particles for 24 h before being irradiated in various doses. The radioprotective properties of nanomelanin after 3 days post-radiation were examined by cell survival rates. Treatment with nanomelanin enhances the percentage of cell viability by 10%, depending on the dose of radiation. At 2 days post-radiation, cell groups treated with nanomelanin were reduced to the transcription levels of gene coding BAX, TNF-α, and Caspase 3 for the cellular apoptosis process at various doses (3–5 Gy) of radiotherapy in comparison with control and eliminated the apoptotic cell rate as analyzed by flow cytometry methods. Furthermore, at 3 Gy, melanin nanoparticles promoted the transcription level gene coding SOD1 enzyme by 3.4 folds compared to control. Graphical abstract

Keywords

  • Nanomelanin,
  • Keratinocyte cells,
  • X-ray exposure,
  • Squid ink

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