10.1007/s40097-022-00507-z

Chitosan/alginate bionanocomposites adorned with mesoporous silica nanoparticles for bone tissue engineering

  • Satar Yousefiasl1,
  • Hamed Manoochehri2,
  • Pooyan Makvandi3,
  • Saeid Afshar2,
  • Erfan Salahinejad4,
  • Pegah Khosraviyan5,
  • Massoud Saidijam2,
  • Sara Soleimani Asl6,
  • Esmaeel Sharifi*7,
  1. School of Dentistry, Hamadan University of Medical Sciences, Hamadan, 6517838736, IR
  2. Research Center for Molecular Medicine, Hamadan University of Medical Sciences, Hamadan, IR
  3. Centre for Materials Interface, Istituto Italiano Di Tecnologia, Pontedera, Pisa, 56025, IT
  4. Faculty of Materials Science and Engineering, K. N. Toosi University of Technology, Tehran, IR
  5. Medical Plants Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, IR
  6. Department of Anatomy, School of Medicine, Hamadan University of Medical Sciences, Hamadan, IR
  7. Research Center for Molecular Medicine, Hamadan University of Medical Sciences, Hamadan, IR Department of Tissue Engineering and Biomaterials, School of Advanced Medical Sciences and Technologies, Hamadan University of Medical Sciences, Hamadan, IR

Published in Issue 25-06-2022

How to Cite

Yousefiasl, S., Manoochehri, H., Makvandi, P., Afshar, S., Salahinejad, E., Khosraviyan, P., Saidijam, M., Soleimani Asl, S., & Sharifi, E. (2022). Chitosan/alginate bionanocomposites adorned with mesoporous silica nanoparticles for bone tissue engineering. Journal of Nanostructure in Chemistry, 13(3 (June 2023). https://doi.org/10.1007/s40097-022-00507-z
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Abstract

Abstract The regeneration of oral and craniofacial bone defects ranging from minor periodontal and peri-implant defects to large and critical lesions imposes a substantial global health burden. Conventional therapies are associated with several limitations, highlighting the development of a unique treatment strategy, such as tissue engineering. A well-designed scaffold for bone tissue engineering should possess biocompatibility, biodegradability, mechanical strength, and osteoconductivity. For this purpose, mesoporous silica nanoparticles (MSNs) were synthesized and incorporated at different ratios (10, 20, and 30%) into alginate/chitosan (Alg/Chit)-based porous composite scaffolds fabricated through the freeze-drying method. The MSN incorporation significantly improved the mechanical strength of the scaffolds while showing a negligible decreasing effect on the porosity. All of the samples showed desirable swelling behaviors, which is beneficial for cell attachment and proliferation. The MSN-containing scaffolds indicated a decreased hydrolytic degradation in an MSN percentage-dependent manner. The fabricated scaffolds did not depict cytotoxic characteristics. The Alg/Chit/MSN30 scaffolds not only showed noncytotoxic properties, but also increased the cell viability significantly compared to the control group. The biomineralization properties of the MSN-containing nanocomposite scaffolds were significantly higher than the Alg/Chit composite, suggesting the potential of these nanoparticles for bone tissue engineering applications. Taken together, it is concluded that the Alg/Chit/MSN30 scaffolds are considerable substances for bone tissue regeneration, and MSN has a great tissue engineering potential in addition to its extensive biomedical applications. Graphical abstract

Keywords

  • Bone substitute,
  • Bone tissue regeneration,
  • Maxillofacial rehabilitation,
  • MSN,
  • Nanocomposites

References

  1. Schmitt et al. (2014) Surgical perspectives in craniofacial trauma (pp. 531-552) https://doi.org/10.1016/j.nic.2014.03.007
  2. Aghali (2021) Craniofacial bone tissue engineering: Current approaches and potential therapy https://doi.org/10.3390/cells10112993
  3. Dave and Gomes (2021) Bioresorbable poly(lactic acid) and organic quantum dot-based nanocomposites: Luminescent scaffolds for enhanced osteogenesis and real-time monitoring (pp. 1-12)
  4. Sepahi et al. (2021) Introducing electrospun polylactic acid incorporating etched halloysite nanotubes as a new nanofibrous web for controlled release of Amoxicillin (pp. 245-258) https://doi.org/10.1007/s40097-020-00362-w
  5. Deka Dey et al. (2022) miRNA-encapsulated abiotic materials and biovectors for cutaneous and oral wound healing: Biogenesis, mechanisms, and delivery nanocarriers https://doi.org/10.1002/btm2.10343
  6. Roseti et al. (2017) Scaffolds for bone tissue engineering: State of the art and new perspectives (pp. 1246-1262) https://doi.org/10.1016/j.msec.2017.05.017
  7. Liu et al. (2013) Review: Development of clinically relevant scaffolds for vascularised bone tissue engineering (pp. 688-705) https://doi.org/10.1016/j.biotechadv.2012.10.003
  8. Agarwal et al. (2021) Recent advances in bioprinting technologies for engineering different cartilage-based tissues https://doi.org/10.1016/j.msec.2021.112005
  9. Islamipour et al. (2022) Biodegradable antibacterial and antioxidant nanocomposite films based on dextrin for bioactive food packaging (pp. 1-16)
  10. Hu et al. (2021) Cytotoxicity of aptamer-conjugated chitosan encapsulated mycogenic gold nanoparticles in human lung cancer cells (pp. 1-13)
  11. Sharifi et al. (2021) Comparison of therapeutic effects of encapsulated Mesenchymal stem cells in Aloe vera gel and Chitosan-based gel in healing of grade-II burn injuries (pp. 30-37) https://doi.org/10.1016/j.reth.2021.02.007
  12. Bagheri et al. (2022) Chitosan nanofiber biocomposites for potential wound healing applications: Antioxidant activity with synergic antibacterial effect https://doi.org/10.1002/btm2.10254
  13. Moeini et al. (2020) Wound healing and antimicrobial effect of active secondary metabolites in chitosan-based wound dressings: A review https://doi.org/10.1016/j.carbpol.2020.115839
  14. Nikbakht et al. (2019) Evaluation of the effects of hyaluronic acid on poly (3-hydroxybutyrate)/chitosan/carbon nanotubes electrospun scaffold: structure and mechanical properties (pp. 2031-2040)
  15. Zafari et al. (2020) Physical and biological properties of blend-electrospun polycaprolactone/chitosan-based wound dressings loaded with N-decyl-N, N -dimethyl-1-decanaminium chloride: An in vitro and in vivo study (pp. 3084-3098) https://doi.org/10.1002/jbm.b.34636
  16. Zahedi et al. (2017) Hydrogels in craniofacial tissue engineering (pp. 47-64) Elsevier https://doi.org/10.1016/B978-0-08-100961-1.00004-9
  17. Abadehie et al. (2021) Lawsone-encapsulated chitosan/polyethylene oxide nanofibrous mat as a potential antibacterial biobased wound dressing (pp. 219-226)
  18. Moukbil et al. (2020) 3D printed bioactive composite scaffolds for bone tissue engineering (pp. 278-314) https://doi.org/10.1016/j.bprint.2019.e00064
  19. Sharifi et al. (2022) Mesoporous bioactive glasses in cancer diagnosis and therapy: Stimuli-responsive, toxicity, immunogenicity, and clinical translation https://doi.org/10.1002/advs.202102678
  20. Kim et al. (2015) Preparation and characterization of nano-sized hydroxyapatite/alginate/chitosan composite scaffolds for bone tissue engineering (pp. 20-25) https://doi.org/10.1016/j.msec.2015.04.033
  21. Eivazzadeh-Keihan et al. (2020) Recent advances in the application of mesoporous silica-based nanomaterials for bone tissue engineering https://doi.org/10.1016/j.msec.2019.110267
  22. Ghosh and Webster (2021) Mesoporous silica based nanostructures for bone tissue regeneration https://doi.org/10.3389/fmats.2021.692309
  23. Lei et al. (2020) Sol–gel-based advanced porous silica materials for biomedical applications https://doi.org/10.1002/adfm.201909539
  24. Acosta Santamaría et al. (2013) Effect of sample pre-contact on the experimental evaluation of cartilage mechanical properties (pp. 911-917) https://doi.org/10.1007/s11340-012-9698-x
  25. Echave et al. (2021) Bioinspired gelatin/bioceramic composites loaded with bone morphogenetic protein-2 (BMP-2) promote osteoporotic bone repair
  26. Assaf et al. (2019) Evaluation of the osteogenic potential of different scaffolds embedded with human stem cells originated from schneiderian membrane: An in vitro study
  27. Purnawira et al. (2019) Synthesis and characterization of mesoporous silica nanoparticles (MSNp) MCM 41 from natural waste rice husk https://doi.org/10.1088/1757-899X/541/1/012018
  28. Kumar and Siril (2018) Enhancing the solubility of fenofibrate by nanocrystal formation and encapsulation (pp. 284-292) https://doi.org/10.1208/s12249-017-0840-z
  29. Wu et al. (2006) “Wet-state” mechanical properties of three-dimensional polyester porous scaffolds (pp. 264-271) https://doi.org/10.1002/jbm.a.30544
  30. Li et al. (2015) Composite mesoporous silica nanoparticle/chitosan nanofibers for bone tissue engineering (pp. 17541-17549) https://doi.org/10.1039/C4RA15232H
  31. Liu et al. (2015) In vitro evaluation of alginate/halloysite nanotube composite scaffolds for tissue engineering (pp. 700-712) https://doi.org/10.1016/j.msec.2015.01.037
  32. Shi et al. (2022) Preparation of the bioglass/chitosan-alginate composite scaffolds with high bioactivity and mechanical properties as bone graft materials https://doi.org/10.1016/j.jmbbm.2021.105062
  33. Zhu et al. (2019) Incorporation of ZnO/bioactive glass nanoparticles into alginate/chitosan composite hydrogels for wound closure (pp. 5042-5052) https://doi.org/10.1021/acsabm.9b00727
  34. Guo et al. (2020) Tuning biodegradability and biocompatibility of mesoporous silica nanoparticles by doping strontium (pp. 11762-11769) https://doi.org/10.1016/j.ceramint.2020.01.210
  35. Bonnans et al. (2014) Remodelling the extracellular matrix in development and disease (pp. 786-801) https://doi.org/10.1038/nrm3904
  36. Lu et al. (2009) Size effect on cell uptake in well-suspended, uniform mesoporous silica nanoparticles (pp. 1408-1413) https://doi.org/10.1002/smll.200900005
  37. Wu et al. (2011) Mesoporous silica nanoparticles as nanocarriers (pp. 9972-9985) https://doi.org/10.1039/c1cc11760b
  38. Li et al. (2019) Mesoporous silica nanoparticles: Synthesis, classification, drug loading, pharmacokinetics, biocompatibility, and application in drug delivery (pp. 219-237) https://doi.org/10.1080/17425247.2019.1575806
  39. Shaheen et al. (2019) Effect of cellulose nanocrystals on scaffolds comprising chitosan, alginate and hydroxyapatite for bone tissue engineering (pp. 814-821) https://doi.org/10.1016/j.ijbiomac.2018.10.081
  40. Sowjanya et al. (2013) Biocomposite scaffolds containing chitosan/alginate/nano-silica for bone tissue engineering (pp. 294-300) https://doi.org/10.1016/j.colsurfb.2013.04.006
  41. Alshatwi et al. (2015) Biocompatibility assessment of rice husk-derived biogenic silica nanoparticles for biomedical applications (pp. 8-16) https://doi.org/10.1016/j.msec.2014.11.005
  42. Shi et al. (2015) Stimulation of osteogenesis and angiogenesis of hBMSCs by delivering Si ions and functional drug from mesoporous silica nanospheres (pp. 178-189) https://doi.org/10.1016/j.actbio.2015.04.019
  43. Li et al. (2013) Effect of ionic products of dicalcium silicate coating on osteoblast differentiation and collagen production via TGF-β1 pathway (pp. 595-604) https://doi.org/10.1177/0885328211416393
  44. Han et al. (2013) The effect of silicate ions on proliferation, osteogenic differentiation and cell signalling pathways (WNT and SHH) of bone marrow stromal cells (pp. 379-392) https://doi.org/10.1039/C2BM00108J
  45. Gu et al. (2011) The stimulation of osteogenic differentiation of human adipose-derived stem cells by ionic products from akermanite dissolution via activation of the ERK pathway (pp. 7023-7033) https://doi.org/10.1016/j.biomaterials.2011.06.003
  46. Yang et al. (2017) The negative effect of silica nanoparticles on adipogenic differentiation of human mesenchymal stem cells (pp. 341-348) https://doi.org/10.1016/j.msec.2017.07.042
  47. Ha et al. (2014) Bio-active engineered 50nm silica nanoparticles with bone anabolic activity: Therapeutic index, effective concentration, and cytotoxicity profile in vitro (pp. 354-364) https://doi.org/10.1016/j.tiv.2013.12.001
  48. Ahmadi et al. (2017) Osteogenic differentiation of mesenchymal stem cells cultured on PLLA scaffold coated with Wharton’s jelly (pp. 785-794)