10.1007/s40097-021-00446-1

Calcium-based nanomaterials and their interrelation with chitosan: optimization for pCRISPR delivery

  • Navid Rabiee1,
  • Mojtaba Bagherzadeh1,
  • Amir Mohammad Ghadiri1,
  • Mahsa Kiani1,
  • Sepideh Ahmadi2,
  • Vahid Jajarmi3,
  • Yousef Fatahi4,
  • Abdullah Aldhaher1,
  • Mohammadreza Tahriri5,
  • Thomas J. Webster6,
  • Ebrahim Mostafavi*7,
  1. Department of Chemistry, Sharif University of Technology, Tehran, IR
  2. Student Research Committee, Department of Medical Biotechnology, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, IR Department of Medical Biotechnology, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, IR
  3. Department of Medical Biotechnology, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, IR Cellular and Molecular Biology Research Center, Shahid Beheshti University of Medical Sciences, Tehran, IR
  4. Department of Pharmaceutical Nanotechnology, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, 14155-6451, IR Faculty of Pharmacy, Nanotechnology Research Center, Tehran University of Medical Sciences, Tehran, 14155-6451, IR Universal Scientific Education and Research Network (USERN), Tehran, 15875-4413, IR
  5. Department of Engineering, Norfolk State University, Norfolk, VA, 23504, US
  6. Department of Chemical Engineering, Northeastern University, Boston, MA, 02115, US
  7. Department of Chemical Engineering, Northeastern University, Boston, MA, 02115, US Stanford Cardiovascular Institute, Stanford University School of Medicine, Stanford, CA, 94305, US Department of Medicine, Stanford University School of Medicine, Stanford, CA, 94305, US

Published in Issue 22-09-2021

How to Cite

Rabiee, N., Bagherzadeh, M., Ghadiri, A. M., Kiani, M., Ahmadi, S., Jajarmi, V., Fatahi, Y., Aldhaher, A., Tahriri, M., Webster, T. J., & Mostafavi, E. (2021). Calcium-based nanomaterials and their interrelation with chitosan: optimization for pCRISPR delivery. Journal of Nanostructure in Chemistry, 12(5 (October 2022). https://doi.org/10.1007/s40097-021-00446-1
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Abstract

Abstract There have been numerous advancements in the early diagnosis, detection, and treatment of genetic diseases. In this regard, CRISPR technology is promising to treat some types of genetic issues. In this study, the relationship between calcium (due to its considerable physicochemical properties) and chitosan (as a natural linear polysaccharide) was investigated and optimized for pCRISPR delivery. To achieve this, different forms of calcium, such as calcium nanoparticles (CaNPs), calcium phosphate (CaP), a binary blend of calcium and chitosan including CaNPs/Chitosan and CaP/Chitosan, as well as their tertiary blend including CaNPs–CaP/Chitosan, were prepared via both routine and green procedures using Salvia hispanica to reduce toxicity and increase nanoparticle stability (with a yield of 85%). Such materials were also applied to the human embryonic kidney (HEK-293) cell line for pCRISPR delivery. The results were optimized using different characterization techniques demonstrating acceptable binding with DNA (for both CaNPs/Chitosan and CaNPs–CaP/Chitosan) significantly enhancing green fluorescent protein (EGFP) (about 25% for CaP/Chitosan and more than 14% for CaNPs–CaP/Chitosan).

Keywords

  • Gene delivery,
  • Calcium-based non-viral vector,
  • pCRISPR,
  • Chitosan-based nanomaterials

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