10.1007/s40097-022-00496-z

Electrochemical immunosensor for the detection of anti-thyroid peroxidase antibody by gold nanoparticles and ionic liquid-modified carbon paste electrode

  • Somayeh Tajik1,
  • Hadi Beitollahi*1,
  • Masoud Torkzadeh-Mahani2,
  1. Environment Department, Institute of Science and High Technology and Environmental Sciences, Graduate University of Advanced Technology, Kerman, IR
  2. Biotechnology Department, Institute of Science and High Technology and Environmental Sciences, Graduate University of Advanced Technology, Kerman, IR

Published in Issue 01-06-2022

How to Cite

Tajik, S., Beitollahi, H., & Torkzadeh-Mahani, M. (2022). Electrochemical immunosensor for the detection of anti-thyroid peroxidase antibody by gold nanoparticles and ionic liquid-modified carbon paste electrode. Journal of Nanostructure in Chemistry, 12(4 (August 2022). https://doi.org/10.1007/s40097-022-00496-z
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Abstract

Abstract In this work, a sandwich-type electrochemical immunosensor was designed based on the modification of the carbon paste electrode consisting of ionic liquid and graphite for the effective detection of anti-thyroid peroxidase antibody (anti-TPO). The ionic liquid carbon paste electrode surface was electrodeposited by biocompatible and electrically conductive gold nanoparticles (NPs) as a sensing platform. The human recombinant TPO (Ab1), anti-TPO antibody (Ag) and horseradish peroxidase (HRP)-anchored anti-TPO secondary antibody (HRP-Ab2) were successively immobilized on the Au NPs/CILE surface to construct the immunosensor. The immunosensor response signal was recorded by differential pulse voltammetry based on HRP catalysis in the reaction of H 2 O 2 with O-aminophenol. An elevation in anti-TPO antibody concentration increased the current responses, probably due to the immobilization of a high amount of HRP-Ab2 on the electrodes for higher anti-TPO antibody concentration. The immunosensor, under the optimized circumstances, presented a low limit of detection (LOD) (6.0 ng mL −1 ) towards the anti-TPO antibody determination with a broad linear range (0.02–60.0 μg mL −1 ). Moreover, the prepared immunosensor showed good reproducibility and good stability.

Keywords

  • Sandwich-type electrochemical immunosensor,
  • Carbon paste electrode,
  • Ionic liquid,
  • Au nanoparticles,
  • Anti-thyroid peroxidase antibody,
  • Gold nanoparticles

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