10.1007/s40097-021-00439-0

Phyto-fabrication of biocompatible silver nanoparticles using Potentilla chinensis Ser leaves: characterization and evaluation of its antibacterial activity

  • Kumar Vishven Naveen1,
  • Hye-Young Kim2,
  • Kandasamy Saravanakumar1,
  • Arokia Vijaya Anand Mariadoss1,
  • Myeong-Hyeon Wang*1,
  1. Department of Bio-Health Convergence, Kangwon National University, Chuncheon, 24341, KR
  2. Department of Dental Hygiene, College of Biomedical Sciences, Kangwon National University, Chuncheon, 200-701, KR

Published in Issue 13-09-2021

How to Cite

Naveen, K. V., Kim, H.-Y., Saravanakumar, K., Mariadoss, A. V. A., & Wang, M.-H. (2021). Phyto-fabrication of biocompatible silver nanoparticles using Potentilla chinensis Ser leaves: characterization and evaluation of its antibacterial activity. Journal of Nanostructure in Chemistry, 12(4 (August 2022). https://doi.org/10.1007/s40097-021-00439-0
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Abstract

Abstract The present study designed to fabricate the silver nanoparticles from the aqueous leaf extracts of Potentilla chinensis Ser (Pc-AgNPs) and their physicochemical characterization were affirmed by spectroscopic and analytical techniques. The absorption maxima at 430 nm verified the synthesis of Pc-AgNPs. Further, the XRD spectroscopy authenticated the crystal, face-centered cubic (fcc) structure of Pc-AgNPs with the 2Ө value of 38.13°, 44.34°, 64.31° and 77.36°. FT-IR spectrum revealed the contribution of functional biomolecules in reduction of Ag + to Ag 0 . Further the zeta potential, particle size and polydispersity index (PDI) of Pc-AgNPs were found as -36.6 ± 0.52 mV, 136 ± 24.82 nm and 0.202, respectively. Besides, the anisotropic structure of Pc-AgNPs with an average size of 24.3 ± 4.6 nm was confirmed by TEM analysis. In vitro antibacterial action of Pc-AgNPs was explored by agar well diffusion, then microtiter method were performed to detect the MIC and MBC of Pc-AgNPs. Further, the cellular changes in bacterial cells were visualized by confocal laser scanning and scanning electron microscopy. Pc-AgNPs considerably inhibited the growth of Salmonella enterica , Staphylococcus aureus , Bacillus cereus and Escherichia coli . The minimum inhibitory concentration (MIC) of Pc-AgNPs was found for S. aureus (5.0 ± 1.15 µg/mL), B. cereus (6.25 ± 0.58 µg/mL), S. enterica (3.75 ± 0.73 µg/mL) and E. coli (3.12 ± 1.25 µg/mL). Besides the biocompatible nature of Pc-AgNPs was explored in HEK-293 cell line, which revealed that up to 500 µg/mL did not cause any significant toxicity. Taken together, our results conclude that Pc-AgNPs have the potential antibacterial activity with promising biocompatibility in HEK-293 cells. Graphic abstract

Keywords

  • Phytosynthesis,
  • Silver nanoparticles,
  • Bacterial disease,
  • Membrane damage,
  • HEK-293 cells,
  • Biocompatibility

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