10.1007/s40089-017-0224-0

Electrosprayed chitosan nanoparticles: facile and efficient approach for bacterial transformation

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  • Morteza Abyadeh1,
  • Esmaeil Sadroddiny2,
  • Ammar Ebrahimi3,
  • Fariba Esmaeili1,
  • Farzaneh Saeedi Landi1,
  • Amir Amani*4,
  1. Department of Medical Nanotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, IR
  2. Department of Medical Biotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, IR
  3. Department of Medical Biotechnology, School of Paramedicine, Guilan University of Medical Sciences, Rasht, IR
  4. Department of Medical Nanotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, IR Medical Biomaterials Research Center, Tehran University of Medical Sciences, Tehran, IR
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Published in Issue 2017-12-28

How to Cite

Abyadeh, M., Sadroddiny, E., Ebrahimi, A., Esmaeili, F., Landi, F. S., & Amani, A. (2017). Electrosprayed chitosan nanoparticles: facile and efficient approach for bacterial transformation. International Nano Letters, 7(4 (December 2017). https://doi.org/10.1007/s40089-017-0224-0
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Abstract

Abstract A rapid and efficient procedure for DNA transformation is a key prerequisite for successful cloning and genomic studies. While there are efforts to develop a facile method, so far obtained efficiencies for alternative methods have been unsatisfactory (i.e. 10 5 –10 6  CFU/μg plasmid) compared with conventional method (up to 10 8  CFU/μg plasmid). In this work, for the first time, we prepared chitosan/pDNA nanoparticles by electrospraying methods to improve transformation process. Electrospray method was used for chitosan/pDNA nanoparticles production to investigate the non-competent bacterial transformation efficiency; besides, the effect of chitosan molecular weight, N/P ratio and nanoparticle size on non-competent bacterial transformation efficiency was evaluated too. The results showed that transformation efficiency increased with decreasing the molecular weight, N/P ratio and nanoparticles size. In addition, transformation efficiency of 1.7 × 10 8  CFU/μg plasmid was obtained with chitosan molecular weight, N/P ratio and nanoparticles size values of 30 kDa, 1 and 125 nm. Chitosan/pDNA electrosprayed nanoparticles were produced and the effect of molecular weight, N/P and size of nanoparticles on transformation efficiency was evaluated. In total, we present a facile and rapid method for bacterial transformation, which has comparable efficiency with the common method.

Keywords

  • Transformation,
  • Chitosan,
  • pDNA,
  • Nanoparticles,
  • Electrospray
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References

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