10.1007/s40089-022-00387-9

Biosynthesis and characterization of silver nanoparticles using Bacillus subtilis, Escherichia coli, and leaf extracts of Jatropha and Ocimum species

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  • F. O. Mbagwu1,
  • S. H. Auta1,
  • M. T. Bankole2,
  • A. S. Kovo3,
  • O. P. Abioye*1,
  1. Department of Microbiology, Federal University of Technology, Minna, NG
  2. Department of Chemistry, Federal University of Technology, Minna, NG
  3. Department of Chemical Engineering, Federal University of Technology, Minna, NG

Published in Issue 2022-11-08

How to Cite

Mbagwu, F. O., Auta, S. H., Bankole, M. T., Kovo, A. S., & Abioye, O. P. (2022). Biosynthesis and characterization of silver nanoparticles using Bacillus subtilis, Escherichia coli, and leaf extracts of Jatropha and Ocimum species. International Nano Letters, 13(1 (March 2023). https://doi.org/10.1007/s40089-022-00387-9
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Abstract

Abstract Silver nanoparticles (AgNPs) are used in many fields for various purposes, and the biosynthesis of AgNPs through biological routes has recently gained attention. In this study, Bacillus subtilis and Escherichia coli isolated from soil within the premises of an abattoir in Minna, Niger State, Nigeria and plant extracts of Tulsi leaves ( Ocimum tenuiflorum ) and Jatropha leaves ( Jatropha curcas ) were evaluated for their ability to synthesize AgNPs. Through visual confirmation and ultraviolet and visible (UV–Vis) spectrum analysis, it was discovered that Bacillus subtilis and Escherichia coli had absorption peak at 425 and 318 nm, respectively, while Jatropha curcas and Ocimum tenuiflorum had absorption peaks at 416 and 400 nm, respectively. X-ray diffraction (XRD) analysis revealed that silver nanoparticles synthesized from B. subtilis and E. coli had a strong peak around 31.66°, which was absent in nanoparticles synthesized with O. tenuiflorum and J. curcas . High-resolution transmission electron microscopy (HRTEM) revealed that the obtained AgNPs had spherical shape and sizes for silver nanoparticles synthesized using B. subtilis and E. coli with an average size of 11.10 ± 0.21 and 38.89 ± 0.42, but the spherical shape of silver nanoparticles in E. coli was evenly distributed compared to the spherical shape in B. subtilis. Nanobars, nanopyramids, nanorods and hexagonal silver nanoparticles were observed in the HRTEM analysis of J. curcas with average size of 12.28 ± 0.37, and nanoflowers were observed in the AgNPs synthesized by O. tenuiflorum with an average size of 12.99 ± 0.15. These results of the study showed that Bacillus subtilis and Escherichia coli as well as plant extracts of Jatropha and Tulsi could be used to produce silver nanoparticles for application in various fields.

Keywords

  • Silver nanoparticles,
  • Nanoflowers,
  • Abattoir,
  • Biosynthesis,
  • Biological routine,
  • Nanorods

References

  1. Amirjani et al. (2020) Predicting the size of silver nanoparticles from their optical properties 15(2) (pp. 1077-1082) https://doi.org/10.1007/s11468-020-01121-x
  2. Acharya et al. (2018) Shape dependent physical mutilation and lethal effects of silver nanoparticles on bacteria 8(1) https://doi.org/10.1038/s41598-017-18590-6
  3. Joshi et al. (2018) Biosynthesis of silver nanoparticles using Carissa carandas berries and its potential antibacterial activities 86(3) (pp. 682-689) https://doi.org/10.1007/s10971-018-4666-2
  4. Guilger-Casagrande and de Lima (2019) Synthesis of silver nanoparticles mediated by fungi: a review 7(2) (pp. 287-290) https://doi.org/10.3389/fbioe.2019.00287
  5. Baghayeri et al. (2018) Green synthesis of silver nanoparticles using water extract of Salvia leriifolia: antibacterial studies and applications as catalysts in the electrochemical detection of nitrite 32(2) (pp. 40-57)
  6. Ansari and Alzohairy (2018) One-pot facile green synthesis of silver nanoparticles using seed extract of Phoenix dactylifera and their bactericidal potential against MRSA 8(20)
  7. Shaik et al. (2018) Plant-extract-assisted green synthesis of silver nanoparticles using Origanum vulgare leaf extract and their microbicidal activities 10(3) (pp. 913-918) https://doi.org/10.3390/su10040913
  8. Bangale and Ghotekar (2019) Bio-fabrication of silver nanoparticles using Rosa chinensis Leave extract for antibacterial activities 10(1) (pp. 217-224)
  9. Hamouda et al. (2019) Synthesis and biological characterization of silver nanoparticles derived from the Cyanobacterium oscillatoria limnetica 9(1) (pp. 1-17) https://doi.org/10.1038/s41598-019-49444-y
  10. Gadkari et al. (2018) Silver nanoparticles in water purification: opportunities and challenges (pp. 229-237) Springer https://doi.org/10.1007/978-3-319-64501-8_13
  11. Bibi et al. (2019) Antibacterial efficacy of silver nanoparticles prepared using Fagonia cretica leaf extract 49(8) (pp. 260-266) https://doi.org/10.1080/24701556.2019.1661440
  12. Li et al. (2018) Antimicrobial nanomaterials for water disinfection and microbial control: potential applications and implications 4(2) (pp. 4591-4602)
  13. Mickymaray (2019) One-step synthesis of silver nanoparticles using Saudi arabian desert seasonal plant Sisymbrium irio and antibacterial activity against multidrug-resistant bacterial strains 9(3) https://doi.org/10.3390/biom9110662
  14. Gong et al. (2018) Development of biosynthesized silver nanoparticles-based formulation for treating wounds during nursing care in hospitals 183(1) (pp. 137-141) https://doi.org/10.1016/j.jphotobiol.2018.04.030
  15. Trease and Evans (1989) (pp. 345-346) ELBS/Bailliere Tindall
  16. Sofowora (1993) Spectrum books Ltd
  17. Siddiqi et al. (2018) A review on biosynthesis of silver nanoparticles and their biocidal properties 16(1) https://doi.org/10.1186/s12951-018-0334-5
  18. Jalal et al. (2019) Anticandidal activity of biosynthesized silver nanoparticles effect on growth cell morphology and key virulence attributes of Candida species 14(5) (pp. 4667-4679) https://doi.org/10.2147/IJN.S210449
  19. Feroze et al. (2019) Fungal mediated synthesis of silver nanoparticles and evaluation of antibacterial activity 6(2) (pp. 231-238)
  20. Baranwal et al. (2018) Prospects of nanostructure materials and their composites as antimicrobial agents 9(2) (pp. 4-22)
  21. Deshmukh et al. (2019) Ultrasound assisted green synthesis of silver and iron oxide nanoparticles using fenugreek seed extract and their enhanced antibacterial and antioxidant activities 7(2) (pp. 17-20)
  22. De Matteis et al. (2019) Cultivar-dependent anticancer and antibacterial properties of silver nanoparticles synthesized using leaves of different Olea europaea trees 9(2) https://doi.org/10.3390/nano9111544
  23. Das et al. (2019) Investigation of antioxidant, antibacterial, antidiabetic, and cytotoxicity potential of silver nanoparticles synthesized using the outer peel extract of Ananas comosus 14(1)
  24. Oves et al. (2019) Antibacterial silver nanomaterial synthesis from Mesoflavibacter zeaxanthinifaciens and targeting biofilm formation 10(2) https://doi.org/10.3389/fphar.2019.00801
  25. Roy et al. (2019) Green synthesis of silver nanoparticles biomolecule–nanoparticle organizations targeting antimicrobial activity 9(3) (pp. 2673-26702) https://doi.org/10.1039/C8RA08982E
  26. Alsharif et al. (2020) Multifunctional properties of spherical silver nanoparticles fabricated by different microbial taxa 6(5) (pp. 23-30) https://doi.org/10.1016/j.heliyon.2020.e03943
  27. Hamida et al. (2020) Synthesisof silver nanoparticles using a novel cyanobacteria Desertifilum sp. extract: theirantibacterial and cytotoxicity effects (pp. 49-63) https://doi.org/10.2147/IJN.S238575
  28. Hossain et al. (2019) Investigation of the antibacterial activity and in vivo cytotoxicity of biogenic silver nanoparticles as potent therapeutics 7(1) https://doi.org/10.3389/fbioe.2019.00239
  29. Modal et al. (2020) Biosynthesis of silver nanoparticles using culture supernatant of Shewanella sp. ARY1 and their antibacterial activity (pp. 8295-8310) https://doi.org/10.2147/IJN.S274535
  30. Shanmuganathan et al. (2019) Synthesis of silver nanoparticles and their biomedical applications—a comprehensive review current 25(2) (pp. 2650-2664)
  31. Torras and Roig (2020) From silver plates to spherical nanoparticles: snapshots of microwave-assisted polyol synthesis 5(3) (pp. 5731-5738) https://doi.org/10.1021/acsomega.9b03748
  32. Neto analytical chemistry, magnetochemistry. Mater. Appl.
  33. 66
  34. (1):173.
  35. Tanase et al. (2019) Antibacterial and antioxidant potential of silver nanoparticles biosynthesized using the spruce bark extract 9(2)
  36. Raman et al. (2022) Edible mushroom extract engineered AgNPs as safe antimicrobial and antioxidant agents with no significant cytotoxicity on human dermal fibroblast cells https://doi.org/10.1016/j.inoche.2022.109362
  37. Senthamarai Murugeswaran and Balasubramanian (2022) Synergistic action of zinc oxide nanoparticle using the unripe fruit extract of Aegle marmelos (L.)—antibacterial, antibiofilm, radical scavenging and ecotoxicological effects https://doi.org/10.1016/j.mtcomm.2022.103228
  38. Sekar et al. (2019) Garlic clove extract assisted silver nanoparticle—antibacterial, antibiofilm, antihelminthic, anti-inflamatory, anticancer and ecotoxicity assessment
  39. Balasubramanian et al. (2017) Two potential uses for silvernanoparticles coated with Solanum nigrum unripe fruit extract: biofilm inhibition and photodegradation of dye effluent (pp. 316-324) https://doi.org/10.1016/j.micpath.2017.08.039