10.1007/s40089-021-00357-7

Electrochemical sensing of cholesterol based on MWCNTs loaded nanoparticles

  • Davood Ghanei Agh Kariz1,
  • Elham Darabi*1,
  • Seyed Mohammad Elahi1,
  1. Plasma Physics Research Center, Science and Research Branch, Islamic Azad University, Tehran, IR

Published in Issue 2021-11-06

How to Cite

Kariz, D. G. A., Darabi, E., & Elahi, S. M. (2021). Electrochemical sensing of cholesterol based on MWCNTs loaded nanoparticles. International Nano Letters, 12(1 (March 2022). https://doi.org/10.1007/s40089-021-00357-7
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Abstract

Abstract A new electrochemical cholesterol biosensor with the basis of gold (Au) and titanium dioxide (TiO 2 ) nanoparticles modified multiwall carbon nanotubes (MWCNTs) and cholesterol oxidase (ChOx) has been successfully synthesized on FTO-coated glass. In this study, the nanocomposites were synthesized and deposited on FTO substrate by sol–gel and dip-coating methods, respectively. Then, the ChOx enzyme immobilized on electrode surface and ChOx/Au/TiO 2 /MWCNTs/FTO was used as the working electrode. The prepared nanocomposites were characterized by X-ray diffraction (XRD), Field emission scanning electron microscopy (FESEM) and Fourier transform infrared spectroscopy (FTIR). To study the electrochemical properties of the electrode, it was subjected through differential pulse voltammetry (DPV), cyclic voltammetry (CV), and electrochemical impedance spectroscopy (EIS) techniques as a function of cholesterol concentration. Simultaneous use of the advantages of Au and TiO 2 nanoparticles and electrochemical techniques has led to enhance the transfer rate of electron results in higher sensitivity The ChOx/Au/TiO 2 /MWCNTs/FTO electrode exhibited excellent analytical linear relationship between the reduction current and cholesterol concentration and low detection limit of 0.0018 μM. This electrochemical biosensor displayed high sensitivity of 50.066 μA/μM which was extracted from DPV data when the range of detection was from 0.05 to 80 µM. After fabrication, the electrode was stable at least for 30 days.

Keywords

  • Electrochemical cholesterol biosensor,
  • ChOx,
  • Gold nanoparticles,
  • Titanium dioxide nanoparticles,
  • Au/TiO2/MWCNTs nanocomposite

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