10.1007/s40089-020-00302-0

Synthesis of mycogenic zinc oxide nanoparticles and preliminary determination of its efficacy as a larvicide against white grubs (Holotrichia sp.)

Show Details XML
  • Gyanika Shukla*1,
  • Shailendra Singh Gaurav1,
  • Amardeep Singh1,
  1. Department of Genetics and Plant Breeding, Chaudhary Charan Singh University, Meerut, UP, IN

Published in Issue 2020-06-05

How to Cite

Shukla, G., Gaurav, S. S., & Singh, A. (2020). Synthesis of mycogenic zinc oxide nanoparticles and preliminary determination of its efficacy as a larvicide against white grubs (Holotrichia sp.). International Nano Letters, 10(2 (June 2020). https://doi.org/10.1007/s40089-020-00302-0
Crossref
Scopus
Google Scholar
Europe PMC

Abstract

Abstract In the present study, the larvicidal efficacy of mycogenic zinc oxide nanoparticles (ZONPs) were tested against white grubs, a potent pest of sugarcane in western Uttar Pradesh (India). The ZONPs were synthesized using  Aspergillus niger  biomass and characterized using UV–Vis spectroscopy, field emission scanning electron microscopy (FESEM), energy dispersive X-ray (EDX), dynamic light scattering (DLS) and Fourier transform infrared (FTIR). the parts per million (ppm) concentration of synthesized ZONPs was established by the inductively coupled plasma mass spectrometry (ICPMS) technique and several ppm dilutions were prepared to determine 50% lethal dose (LD50). The UV–Vis spectroscopy showed peaks at 240, 290, 340, and 380 nm, corresponding to ZONPs. The FESEM results also confirmed the synthesis of nano-sized particles. EDX analysis result showed the optical absorption peaks specific to ZONPs. The DLS result confirmed the synthesis of ZONPs with sizes ranging from 76.2 to 183.8 nm. The FTIR spectrum analysis confirmed the presence of various functional group interactions in the nanoparticle sample. The ZONPs were tested against the first instar larvae of white grubs. The LD 50 was calculated to be 12.63 ppm which still needs to be validated for significance. In the near future, we are planning to establish the minimal lethal dosage of ZONPs to prepare effective larvicidal formulations against white grub infection with minimal toxicity to the environment.

Keywords

  • Zinc oxide nanoparticles,
  • Myconanoparticles,
  • Nanopesticides,
  • Aspergillus niger,
  • Holotrichia sp.,
  • White grub,
  • Lethal dosage,
  • Nanoparticle toxicity,
  • EDX,
  • FESEM,
  • DLS,
  • FTIR,
  • ICPMS

References

  1. Al-Dhabi and Valan Arasu (2018) Environmentally-friendly green approach for the production of zinc oxide nanoparticles and their anti-fungal, ovicidal, and larvicidal properties 8(7)
  2. Andrade-Ochoa et al. (2018) Oviposition deterrent and larvicidal and pupaecidal activity of seven essential oils and their major components against Culex quinquefasciatus say (Diptera: Culicidae): synergism–antagonism effects 9(1)
  3. Anitha et al. (2005) Management of white grubs (Coleoptera: Scarabaeidae) on groundnut in southern India 51(4) (pp. 313-320)
  4. Arciniegas-Grijalba et al. (2017) ZnO nanoparticles (ZnO-NPs) and their antifungal activity against coffee fungus Erythricium salmonicolor 7(5) (pp. 225-241)
  5. Babu et al. (2014) Application of biosynthesized silver nanoparticles in agricultural and marine pest control 10(3) (pp. 374-381)
  6. Banumathi et al. (2016) Invitro acaricidal activity of ethnoveterinary plants and green synthesis of zinc oxide nanoparticles against Rhipicephalus (Boophilus) microplus (pp. 93-100)
  7. Bhawane et al. (2011) Life cycle of Holotrichia karschi Brenske (Coleoptera: Scarabaeidae: Melolonthinae) 6(3) (pp. 471-474)
  8. Chandler et al. (2011) The development, regulation and use of biopesticides for integrated pest management 366(1573) (pp. 1987-1998)
  9. Chikkanna et al. (2019) Green synthesis of Zinc oxide nanoparticles (ZnO NPs) and their biological activity 1(1)
  10. Christian et al. (2008) Nanoparticles: structure, properties, preparation and behaviour in environmental media 17(5) (pp. 326-343)
  11. Dadmal and Khadakkar (2014) Revision of Holotrichia hope (Scarabaeidae: Melolonthinae) in different agro–climatic zones of Maharashtra (India) 2(3) (pp. 50-58)
  12. Devi and Gayathri (2010) FTIR and FT-Raman spectral analysis of paclitaxel drugs 2(2) (pp. 106-110)
  13. Dhoj (2009) Microbial control of white grubs in Nepal: the way forward (pp. 134-142)
  14. Dorcheh and Vahabi (2016) (pp. 1-20) Springer
  15. Erler and Ates (2015) Potential of two entomopathogenic fungi, Beauveria bassiana and Metarhizium anisopliae (Coleoptera: Scarabaeidae), as biological control agents against the June beetle 15(1)
  16. Evanoff and Chumanov (2005) Synthesis and optical properties of silver nanoparticles and arrays 6(7) (pp. 1221-1231)
  17. Ezealisiji et al. (2019) Green synthesis of zinc oxide nanoparticles using Solanum torvum (L.) leaf extract and evaluation of the toxicological profile of the ZnO nanoparticles–hydrogel composite in Wistar albino rats 9(2) (pp. 99-107)
  18. Fakhari et al. (2019) Green synthesis of zinc oxide nanoparticles: a comparison 12(1) (pp. 19-24)
  19. Gandhi et al. (2017) Acaricidal, pediculicidal and larvicidal activity of synthesized ZnO nanoparticles using Momordica charantia leaf extract against blood feeding parasites (pp. 47-56)
  20. Getie et al. (2017) Synthesis and characterizations of zinc oxide nanoparticles for antibacterial applications
  21. Ghareib et al. (2018) Eco-friendly approach for biosynthesis of zinc oxide nanoparticles using some soil fungi 7(3) (pp. 108-118)
  22. Hao and Schatz (2004) Electromagnetic fields around silver nanoparticles and dimers 120(1) (pp. 357-366)
  23. Inglis et al. (2001) (pp. 23-69) Springer
  24. Jacob et al. (2014) Aspergillus niger mediated synthesis of ZnO nanoparticles and their antimicrobial and in vitro anticancerous activity 3(2) (pp. 3044-3054)
  25. Jans et al. (2009) Dynamic light scattering as a powerful tool for gold nanoparticle bioconjugation and biomolecular binding studies 81(22) (pp. 9425-9432)
  26. Kalpana et al. (2018) Biosynthesis of zinc oxide nanoparticles using culture filtrates of aspergillus niger: antimicrobial textiles and dye degradation studies (pp. 48-55)
  27. Kheswa, N.: Development of beauveria brongniartii as a bio-Insecticide to control white grub (coleoptera: scarabaeidae) species attacking sugarcane in South Africa (Doctoral dissertation) (2016)
  28. Khlebtsov and Dykman (2011) Biodistribution and toxicity of engineered gold nanoparticles: a review of in vitro and in vivo studies 40(3) (pp. 1647-1671)
  29. Khooshe-Bast et al. (2016) Insecticidal effects of zinc oxide nanoparticles and Beauveria bassiana TS11 on Trialeurodes vaporariorum (Westwood, 1856) (Hemiptera: Aleyrodidae) 107(2) (pp. 299-309)
  30. Kirthi et al. (2011) Acaricidal, pediculocidal and larvicidal activity of synthesized ZnO nanoparticles using wet chemical route against blood feeding parasites 109(2) (pp. 461-472)
  31. Kumar et al. (2013) Synthesis, characterization and optical properties of zinc oxide nanoparticles 3(1)
  32. la Rosa-García et al. (2018) Antifungal activity of ZnO and MgO nanomaterials and their mixtures against Colletotrichum gloeosporioides strains from tropical fruit https://doi.org/10.1155/2018/3498527
  33. Maksymiv (2015) Pesticides: benefits and hazards 2(1) (pp. 70-76)
  34. Malaikozhundan and Vinodhini (2018) Nanopesticidal effects of Pongamia pinnata leaf extract coated zinc oxide nanoparticle against the Pulse beetle, Callosobruchus maculatus (pp. 106-115)
  35. Mane and Mohite (2014) Bioefficacy of different species of entomopathogenic fungi against white grub, Leucopholis lepidophora (Blanchard) infesting sugarcane in Maharashtra 9(2) (pp. 234-237)
  36. Meruvu et al. (2011) Synthesis and characterization of zinc oxide nanoparticles and its antimicrobial activity against Bacillus subtilis and Escherichia coli 4(1) (pp. 217-222)
  37. Mohan and Renjanadevi (2016) Preparation of zinc oxide nanoparticles and its characterization using scanning electron microscopy (SEM) and X-ray diffraction (XRD) (pp. 761-766)
  38. Morejón et al. (2018) Larvicidal activity of silver nanoparticles synthesized using extracts of Ambrosia arborescens (Asteraceae) to Control Aedes aegypti L. (Diptera: Culicidae) https://doi.org/10.1155/2018/6917938
  39. Murugan et al. (2018) Sargassum wightii-synthesized ZnO nanoparticles reduce the fitness and reproduction of the malaria vector Anopheles stephensi and cotton bollworm Helicoverpa armigera (pp. 202-213)
  40. Nagarajan and Kuppusamy (2013) Extracellular synthesis of zinc oxide nanoparticle using seaweeds of gulf of Mannar India 11(1)
  41. Nataraja, M.V., Jadon, K.S., Dutta, R., Savalia, S.D.: White grubs and their management in groundnut. Web report, Indian Council of Agricultural Research
  42. Oancea et al. (2012) Laboratory infrared reflection spectrum of carbon dioxide clathrate hydrates for astrophysical remote sensing applications 221(2) (pp. 900-910)
  43. Oliveira et al. (2000) Effect of soil management on the white grub population and damage in soybean 35(5) (pp. 887-894)
  44. Pathania et al. (2016) Seasonal life cycle of Holotrichia longipennis (Blanchard) (Coleoptera: Scarabaeidae: Melolonthinae): a serious foliage and root feeding pest in India 44(5) (pp. 615-629)
  45. Patil and Raut (2012) Synthesis and characterization of ZnO nanoparticles and 50% ZnO-bentonite nanocomposite 10(2) (pp. 1124-1132)
  46. Rajan et al. (2016) Biosynthesis of zinc oxide nanoparticles using Aspergillus fumigatus JCF and its antibacterial activity 1(2) (pp. 52-57)
  47. Resham et al. (2015) Nanobiotechnology in agricultural development (pp. 683-698) Springer
  48. Revina et al. (2007) Synthesis and properties of zinc nanoparticles: the role and prospects of radiation chemistry in the development of modern nanotechnology 4(3) (pp. 613-618)
  49. Samson et al. (2005) Effect of fungicides and insecticides applied during planting of sugarcane on viability of Metarhizium anisopliae and its efficacy against white grubs 50(1) (pp. 151-163)
  50. Santhoshkumar et al. (2017) Synthesis of zinc oxide nanoparticles using plant leaf extract against urinary tract infection pathogen 3(4) (pp. 459-465)
  51. Shaheen et al. (2016) Durable antibacterial and UV protections of in situ synthesized zinc oxide nanoparticles onto cotton fabrics (pp. 426-432)
  52. Shanmugasundaram and Balagurunathan (2015) Mosquito larvicidal activity of silver nanoparticles synthesised using actinobacterium, Streptomyces sp. M25 against Anopheles subpictus, Culex quinquefasciatus and Aedes aegypti 39(4) (pp. 677-684)
  53. Shi et al. (2012) Nanoparticles as delivery vehicles for sunscreen agents (pp. 122-129)
  54. Shukla et al. (2017) Nanotechnology: an innovative approach for waste water treatment https://doi.org/10.1007/978-3-030-02381-2_5
  55. Singh et al. (2010) Biosynthesis of gold and silver nanoparticles by natural precursor clove and their functionalization with amine group 12(5) (pp. 1667-1675)
  56. Sreedevi and Tyagi (2015) Species diversity of white grubs associated with sugarcane ecosystem of western Uttar Pradesh. A case study 8(4) (pp. 404-410)
  57. Srivastava et al. (1971) On the life history of white grub, holotrichia consanguinea Blanch (Coleopt, Melolonthidae) 68(1–4) (pp. 154-157)
  58. Stadler et al. (2010) Novel use of nanostructured alumina as an insecticide 66(6) (pp. 577-579)
  59. Sutthanont et al. (2019) Larvicidal activity of synthesized silver nanoparticles from Curcuma zedoaria essential oil against Culex quinquefasciatus 10(1)
  60. Talebian and Nilforoushan (2010) Comparative study of the structural, optical and photocatalytic properties of semiconductor metal oxides toward degradation of methylene blue 518(8) (pp. 2210-2215)
  61. Theurkar et al. (2013) New laboratory culture method for white grub national pest, India 2(5) (pp. 83-85)
  62. Tiwari et al. (2018) Mechanism of anti-bacterial activity of zinc oxide nanoparticle against carbapenem-resistant Acinetobacter baumannii
  63. Tso et al. (2010) Stability of metal oxide nanoparticles in aqueous solutions 61(1) (pp. 127-133)
  64. Umar et al. (2019) Biosynthesis of zinc oxide nanoparticles using Albizia lebbeck stem bark, and evaluation of its antimicrobial, antioxidant, and cytotoxic activities on human breast cancer cell lines
  65. Xie et al. (2011) Antibacterial activity and mechanism of action of zinc oxide nanoparticles against Campylobacter jejuni 77(7) (pp. 2325-2331)
  66. Yang et al. (2009) Structural characterization of nanoparticles loaded with garlic essential oil and their insecticidal activity against Tribolium castaneum (Herbst) (Coleoptera: Tenebrionidae) (pp. 10156-10162)
  67. Yusof et al. (2019) Microbial synthesis of zinc oxide nanoparticles and their potential application as an antimicrobial agent and a feed supplement in animal industry: a review 10(1)
  68. Zanetti-Ramos et al. (2009) Dynamic light scattering and atomic force microscopy techniques for size determination of polyurethane nanoparticles 29(2) (pp. 638-640)