10.1186/2228-5326-3-36

In vitro anticancer evaluation of 5-fluorouracil lipid nanoparticles using B16F10 melanoma cell lines

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  • Vikram S Shenoy*1,
  • Rajiv P Gude2,
  • Rayasa S Ramachandra Murthy1,
  1. Department Pharmacy, M S University of Baroda, Gujarat, 390 002, IN
  2. Department of Chemotherapy, Tata Memorial Center, Cancer Research Institute, Navi Mumbai, 410208, IN
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Published in Issue 2013-05-17

How to Cite

Shenoy, V. S., Gude, R. P., & Murthy, R. S. R. (2013). In vitro anticancer evaluation of 5-fluorouracil lipid nanoparticles using B16F10 melanoma cell lines. International Nano Letters, 3(1 (December 2013). https://doi.org/10.1186/2228-5326-3-36
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Abstract

Abstract The present study is aimed to investigate the formulation and in vitro anticancer activities of solid lipid nanoparticles (SLNs) of 5-fluorouracil (5-FU) prepared using glyceryl monostearate (GMS) and cetyl palmitate (CP) by hot homogenization method. The lipids were selected based on the partition coefficient of 5-FU in lipids. The lipid nanoparticles were optimized for process and formulation parameters. The optimized nanoparticles were characterized for their zeta potential, morphology, release kinetics, and anticancer activity. Higher entrapments were achieved using a combination of emulsifiers. The zeta potential of the optimized CP and GMS SLN formulation were −8.26 and −9.35 mV, respectively. Both the optimized formulations were spherical. The in vitro release studies of SLNs of both the lipid carriers followed Peppas-Korsenmeyer equation when carried out at pH 3.5 and 7.4. The chemosensitivity assay carried out in B16F10 cell lines revealed that CP SLNs had better cytotoxicity than 5-FU solution and GMS SLNs at 48 h of incubation. Subtoxic concentration of 5-FU-loaded CP SLNs (0.12 μg/mL) possessed comparable antimigrational activity, colony inhibition activity, and cytopathic as that of 5-FU solution effects. The results indicated that encapsulating 5-FU in CP would be a promising delivery system for delivering 5-FU.

Keywords

  • Cell morphology,
  • Cetyl palmitate,
  • Chemosensitivity assay,
  • Colony formation,
  • Glyceryl monostearate,
  • Hot homogenization
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