10.1186/2228-5326-2-18

Piper betle-mediated green synthesis of biocompatible gold nanoparticles

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  • Jayasekhar Babu Punuri1,
  • Pragya Sharma2,
  • Saranya Sibyala3,
  • Ranjan Tamuli2,
  • Utpal Bora*1,
  1. Biomaterials and Tissue Engineering Laboratory, Department of Biotechnology, Indian Institute of Technology Guwahati, Guwahati, Assam, 781039, IN Biotech Hub, Centre for the Environment, Indian Institute of Technology Guwahati, Guwahati, Assam, 781039, IN
  2. Biotech Hub, Centre for the Environment, Indian Institute of Technology Guwahati, Guwahati, Assam, 781039, IN
  3. Biotech Hub, Centre for the Environment, Indian Institute of Technology Guwahati, Guwahati, Assam, 781039, IN Department of Biotechnology, D.R.W. University, SPS Nellore, Andhra Pradesh, 524101, IN
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Published in Issue 2012-08-30

How to Cite

Punuri, J. B., Sharma, P., Sibyala, S., Tamuli, R., & Bora, U. (2012). Piper betle-mediated green synthesis of biocompatible gold nanoparticles. International Nano Letters, 2(1 (December 2012). https://doi.org/10.1186/2228-5326-2-18
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Abstract

Abstract Here, we report the novel use of the ethonolic leaf extract of Piper betle for gold nanoparticle (AuNP) synthesis. The successful formation of AuNPs was confirmed by UV-visible spectroscopy, and different parameters such as leaf extract concentration (2%), gold salt concentration (0.5 mM), and time (18 s) were optimized. The synthesized AuNPs were characterized with different biophysical techniques such as transmission electron microscopy (TEM), Fourier transform infrared (FT-IR) spectroscopy, X-ray diffraction (XRD), and energy-dispersive X-ray spectroscopy (EDX). TEM experiments showed that nanoparticles were of various shapes and sizes ranging from 10 to 35 nm. FT-IR spectroscopy revealed that AuNPs were functionalized with biomolecules that have primary amine group –NH 2 , carbonyl group, –OH groups, and other stabilizing functional groups. EDX showed the presence of the elements on the surface of the AuNPs. FT-IR and EDX together confirmed the presence of biomolecules bounded on the AuNPs. Cytotoxicity of the AuNPs was tested on HeLa and MCF-7 cancer cell lines, and they were found to be nontoxic, indicating their biocompatibility. Thus, synthesized AuNPs have potential for use in various biomedical applications.

Keywords

  • Nanocrystalline materials,
  • Biomaterials,
  • Crystal growth,
  • Electron microscopy,
  • Fourier transform infrared spectroscopy,
  • Biosynthesis,
  • Nucleation
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