10.1007/s40089-014-0116-5

Implications of the stability behavior of zinc oxide nanoparticles for toxicological studies

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  • Tobias Meißner*1,
  • Kathrin Oelschlägel1,
  • Annegret Potthoff1,
  1. Fraunhofer Institute for Ceramic Technologies and Systems, Dresden, 01277, DE
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Published in Issue 2014-08-30

How to Cite

Meißner, T., Oelschlägel, K., & Potthoff, A. (2014). Implications of the stability behavior of zinc oxide nanoparticles for toxicological studies. International Nano Letters, 4(3 (September 2014). https://doi.org/10.1007/s40089-014-0116-5
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Abstract

Abstract The increasing use of zinc oxide (ZnO) nanoparticles in sunscreens and other cosmetic products demands a risk assessment that has to be done in toxicological studies. Such investigations require profound knowledge of the behavior of ZnO in cell culture media. The current study was performed to get well-dispersed suspensions of a hydrophilic (ZnO-hydro) and a lipophilic coated (ZnO-lipo) ZnO nanomaterial for use in in vitro tests. Therefore, systematic tests were carried out with common dispersants (phosphate, lecithin, proteins) to elucidate chemical and physical changes of ZnO nanoparticles in water and physiological solutions (PBS, DMEM). Non-physiological stock suspensions were prepared using ultrasonication. Time-dependent changes of pH, conductivity, zeta potential, particle size and dissolution were recorded. Secondly, the stock suspensions were added to physiological media with or without albumin (BSA) or serum (FBS), to examine characteristics such as agglomeration and dissolution. Stable stock suspensions were obtained using phosphate as natural and physiological electrostatic stabilizing agent. Lecithin proved to be an effective wetting agent for ZnO-lipo. Although the particle size remained constant, the suspension changed over time. The pH increased as a result of ZnO dissolution and formation of zinc phosphate complexes. The behavior of ZnO in physiological media was found to depend strongly on the additives used. Applying only phosphate as additive, ZnO-hydro agglomerated within minutes. In the presence of lecithin or BSA/serum, agglomeration was inhibited. ZnO dissolution was higher under physiological conditions than in the stock suspension. Serum especially promoted this process. Using body-related dispersants (phosphate, lecithin) non-agglomerating stock suspensions of hydrophilic and lipophilic ZnO were prepared as a prerequisite to perform meaningful toxicological investigation. Both nanomaterials showed a non-negligible dissolution behavior that strongly depended on the surrounding conditions. Agglomeration of ZnO particles in physiological media is a complex function of particle coating, used dispersants and serum proteins if supplemented. The present study gives a clear guideline how to prepare and handle suspensions with ZnO for in vitro testing and allows the correlation between the chemical-physical particles behavior with findings from toxicological tests.

Keywords

  • Zinc oxide,
  • Nanoparticles,
  • Toxicity,
  • Dispersion,
  • Dissolution,
  • Particle agglomeration
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References

  1. Osmond and McCall (2010) Zinc oxide nanoparticles in modern sunscreens: an analysis of potential exposure and hazard 4(1) (pp. 15-41) https://doi.org/10.3109/17435390903502028
  2. Oberdörster et al. (2005) Nanotoxicology: an emerging discipline evolving from studies of ultrafine particles 113(7) (pp. 823-839) https://doi.org/10.1289/ehp.7339
  3. Dhawan, A, Sharma, V, Parmar, D. Nanomaterials: a challenge for toxicologists. Nanotoxicology
  4. 3
  5. (1), 1–9 (2009)
  6. Hsiao and Huang (2011) Effects of various physicochemical characteristics on the toxicities of ZnO and TiO2 nanoparticles toward human lung epithelial cells (pp. 1219-1228) https://doi.org/10.1016/j.scitotenv.2010.12.033
  7. Lin et al. (2008) Toxicity of nano- and micro-sized ZnO particles in human lung epithelial cells 2008(11) (pp. 23-39)
  8. Xia et al. (2008) Comparison of the mechanism of toxicity of zinc oxide and cerium oxide nanoparticles based on dissolution and oxidative stress properties (pp. 2121-2134) https://doi.org/10.1021/nn800511k
  9. Pujalté et al. (2011) Cytotoxicity and oxidative stress induced by different metallic nanoparticles on human kidney cells https://doi.org/10.1186/1743-8977-8-10
  10. Brunner et al. (2006) In vitro cytotoxicity of oxide nanoparticles: comparison to asbestos, silica, and the effect of particle solubility 40(14) (pp. 4374-4381) https://doi.org/10.1021/es052069i
  11. Corradi et al. (2012) Influence of serum on in situ proliferation and genotoxicity in A549 human lung cells exposed to nanomaterials (pp. 21-27) https://doi.org/10.1016/j.mrgentox.2011.10.007
  12. Cho et al. (2011) Progressive severe lung injury by zinc oxide nanoparticles; the role of Zn2+ dissolution inside lysosomes https://doi.org/10.1186/1743-8977-8-27
  13. Osaka et al. (2009) Effect of surface charge of magnetite nanoparticles on their internalization into breast cancer and umbilical vein endothelial cells (pp. 325-330) https://doi.org/10.1016/j.colsurfb.2009.03.004
  14. Murdock et al. (2008) Characterization of nanomaterial dispersion in solution prior to in vitro exposure using dynamic light scattering technique 101(2) (pp. 239-253) https://doi.org/10.1093/toxsci/kfm240
  15. Berg et al. (2009) The relationship between pH and zeta potential of ~30 nm metal oxide nanoparticle suspensions relevant to in vitro toxicological evaluations 3(4) (pp. 276-283) https://doi.org/10.3109/17435390903276941
  16. Cho et al. (2012) Zeta potential and solubility to toxic ions as mechanisms of lung inflammation caused by metal/metal-oxide nanoparticles (pp. 469-477) https://doi.org/10.1093/toxsci/kfs006
  17. Nel et al. (2009) Understanding biophysicochemical interactions at the nano-bio interface (pp. 543-557) https://doi.org/10.1038/nmat2442
  18. Horie et al. (2009) Protein adsorption of ultrafine metal oxide and its influence on cytotoxicity toward cultured cells (pp. 543-553) https://doi.org/10.1021/tx800289z
  19. Kathiravan et al. (2009) Study on the binding of colloidal zinc oxide nanoparticles with bovine serum albumin (pp. 129-137) https://doi.org/10.1016/j.molstruc.2009.06.032
  20. Monopoli et al. (2012) Biomolecular coronas provide the biological identity of nanosized materials (pp. 779-786) https://doi.org/10.1038/nnano.2012.207
  21. Casals et al. (2011) Hardening of the nanoparticle-protein corona in metal (Au, Ag) and oxide (Fe3O4, CoO, and CeO2) nanoparticles 7(24) (pp. 3479-3486) https://doi.org/10.1002/smll.201101511
  22. Powers et al. (2006) Research strategies for safety evaluation of nanomaterials. Part VI. Characterization of nanoscale particles for toxicological evaluation 90(2) (pp. 296-303) https://doi.org/10.1093/toxsci/kfj099
  23. Teeguarden et al. (2007) Particokinetics in vitro: dosimetry Considerations for in vitro nanoparticle toxicity assessments 95(2) (pp. 300-312) https://doi.org/10.1093/toxsci/kfl165
  24. Warheit (2008) How meaningful are the results of nanotoxicity studies in the absence of adequate material characterization? 101(2) (pp. 183-185) https://doi.org/10.1093/toxsci/kfm279
  25. Bihari et al. (2008) Optimized dispersion of nanoparticles for biological in vitro and in vivo studies https://doi.org/10.1186/1743-8977-5-14
  26. Taurozzi et al. (2013) A standardised approach for the dispersion of titanium dioxide nanoparticles in biological media 7(4) (pp. 389-401) https://doi.org/10.3109/17435390.2012.665506
  27. Meißner et al. (2010) Physical-chemical characterization of tungsten carbide nanoparticles as a basis for toxicological investigations 4(2) (pp. 196-206) https://doi.org/10.3109/17435391003605455
  28. Meißner et al. (2010) Physico-chemical characterization in the light of toxicological effects (pp. 35-39) https://doi.org/10.1080/08958370902942608
  29. Potthoff et al. (2009) Evaluation of health risks of nanoparticles—a contribution to a sustainable development of nanotechnology (pp. 183-189) https://doi.org/10.4028/www.scientific.net/SSP.151.183
  30. Bastian et al. (2009) Toxicity of tungsten carbide and cobalt-doped tungsten carbide nanoparticles in mammalian cells in vitro 117(4) (pp. 530-536) https://doi.org/10.1289/ehp.0800121
  31. Malvern Instruments.: Sample and dispersion refractive index guide. Man0396, Issue 1.0 (2007)
  32. International Organisation for Standardization. ISO 14887:2000. Sample preparation—dispersing procedures for powders in liquids (2000)
  33. Müller, R.H.: Zetapotential und Partikelladung in der Laborpraxis. APV paperback series, vol. 37. Wissenschaftliche Verlagsgesellschaft, Stuttgart (1996)
  34. Jiang et al. (2009) Characterization of size, surface charge, and agglomeration state of nanoparticle dispersions for toxicological studies (pp. 77-89) https://doi.org/10.1007/s11051-008-9446-4
  35. Sager, T.M., Porter, D.W., Robinson, V.A., Lindsley, W.G., Schwegler-Berry, D.E., Castranova, V.: Improved method to disperse nanoparticles for in vitro and in vivo investigation of toxicity. Nanotoxicology
  36. 1
  37. (2), 188–129 (2007)
  38. Porter et al. (2008) A biocompatible medium for nanoparticle dispersion 2(3) (pp. 144-154) https://doi.org/10.1080/17435390802318349
  39. Dimkpa et al. (2011) Responses of a soil bacterium, Pseudomonas chlororaphis O6 to commercial metal oxide nanoparticles compared with responses to metal ions 159(7) (pp. 1749-1756) https://doi.org/10.1016/j.envpol.2011.04.020
  40. Fruhwirth et al. (1982) ZnO dissolution kinetics by means of a 65Zn tracer method (pp. 43-50) https://doi.org/10.1016/0376-4583(82)90083-8
  41. Michelmore et al. (2003) The interaction of linear polyphosphates with zincite surfaces (pp. 1-16) https://doi.org/10.1016/S0301-7516(01)00085-0
  42. Healy and Ducheyne (1992) Hydration and preferential molecular adsorption on titanium in vitro 13(8) (pp. 553-561) https://doi.org/10.1016/0142-9612(92)90108-Z
  43. Allouni et al. (2009) Agglomeration and sedimentation of TiO2 nanoparticles in cell culture medium (pp. 83-87) https://doi.org/10.1016/j.colsurfb.2008.09.014
  44. Baalousha (2009) Aggregation and disaggregation of iron oxide nanoparticles: influence of particle concentration, pH and natural organic matter 407(6) (pp. 2093-2101) https://doi.org/10.1016/j.scitotenv.2008.11.022
  45. Kosmulski and Schick (2001) Chemical Properties of Material Surfaces Marcel Decker Inc.
  46. Chibowski et al. (2007) Influence of ionic surfactants and lecithin on stability of titanium dioxide in aqueous electrolyte solution (pp. 395-403)
  47. Buford et al. (2007) A comparison of dispersing media for various engineered carbon nanoparticles https://doi.org/10.1186/1743-8977-4-6
  48. Tantra et al. (2010) Characterisation of the de-agglomeration effects of bovine serum albumin on nanoparticles in aqueous suspension (pp. 275-281) https://doi.org/10.1016/j.colsurfb.2009.08.049
  49. Limbach et al. (2005) Oxide nanoparticle uptake in human lung fibroblasts: effects of particle size, agglomeration, and diffusion at low concentrations 39(23) (pp. 9370-9376) https://doi.org/10.1021/es051043o
  50. Landsiedel et al. (2010) Testing metal-oxide nanomaterials for human safety 2010(22) (pp. 2601-2627) https://doi.org/10.1002/adma.200902658
  51. Mahon et al. (2012) Stabilising fluorescent silica nanoparticles against dissolution effects for biological studies 48(64) (pp. 7970-7972) https://doi.org/10.1039/c2cc34023b