10.57647/j.ijc.2025.1501.11

Facile synthesis of silver/iron-TiO2 nanoparticles for sterilization of Nodal explants of Dracaena sanderiana Sander ex Mast. cvs ‘Gold’ and ‘Victory’ and growth regulator effects on in vitro growth

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  • Kasun L. Seneviratne1,
  • Sriyani E. Peiris2,
  • Colin N. Peiris*2,
  • R.P.A. Shashikala3,
  • Sehan Jayasinghe3,
  • Nilwala Kottegoda4,
  1. Integrated Watershed and Water Resources Management Project (IWWRMP), Ministry of Irrigation, Colombo, Sri Lanka
  2. Faculty of Humanities and Sciences, Sri Lanka Institute of Information Technology (SLIIT), New Kandy Road, Malabe, Sri Lanka
  3. Faculty of Humanities and Sciences, Sri Lanka Institute of Information Technology (SLIIT), New Kandy Road, Malabe, Sri Lanka
  4. Department of Chemistry, Faculty of Applied Sciences, University of Sri Jayewardenepura, Colombo, Sri Lanka
Facile synthesis of silver/iron-TiO2 nanoparticles for sterilization of Nodal explants of Dracaena sanderiana Sander ex Mast. cvs ‘Gold’ and ‘Victory’ and growth regulator effects on in vitro growth

Received: 2024-08-11

Revised: 2024-11-27

Accepted: 2025-01-27

Published 2025-02-08

Copyright (c) 2025 Kasun L. Seneviratne, Sriyani E. Peiris, Colin N. Peiris, R.P.A. Shashikala, Sehan Jayasinghe, Nilwala Kottegoda (Author)

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This work is licensed under a Creative Commons Attribution 4.0 International License.

How to Cite

Seneviratne, K. L., Peiris, S. E., Peiris, C. N., Shashikala, R., Jayasinghe, S., & Kottegoda, N. (2025). Facile synthesis of silver/iron-TiO2 nanoparticles for sterilization of Nodal explants of Dracaena sanderiana Sander ex Mast. cvs ‘Gold’ and ‘Victory’ and growth regulator effects on in vitro growth. Iranian Journal of Catalysis, 15(1 (March 2025). https://doi.org/10.57647/j.ijc.2025.1501.11
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Abstract

This study investigates the application of silver/iron-titanate (Ag/Fe-TiO2) nanoparticles (NPs) as a sterilizing agent for in vitro cultures of Dracaena sanderiana cultivars ‘Gold’ and ‘Victory’. The motivation behind this research is to find an effective sterilization method that does not harm plant growth. The Ag/Fe-TiO2 NPs, activated by visible light, exhibit catalytic properties that eliminate harmful microbes such as Escherichia coli, Staphylococcus aureus, and Fusarium spp. The main findings indicate that the combination of Benzyl Amino Purine (BAP) at 1.5 mg L-1 and Indole Acetic Acid (IAA) at 0.01 mg L-1 significantly enhances shoot number and length in both cultivars. The use of 200 mg L-1 Ag/Fe-TiO2 NPs achieved 90% contamination-free cultures in the first cycle, with a slight decrease to 80% in the second cycle and 70% in the third cycle. Importantly, the treatment not only sterilizes but also promotes plant growth, suggesting that Ag/Fe-TiO2 NPs could be a sustainable solution for in vitro plant mass production.

Research Highlights

  • Visible-light-driven Ag/Fe-TiO2 NPs show significant antibacterial and antifungal activity.
  • Antimicrobial action of Ag/Fe-TiO2 NPs is utilized for in vitro micro-propagation.
  • The proposed Ag/Fe-TiO2 NPs treatment eliminates the bacteria and fungus sp. adhere to the plant node surface.
  • The growth regulator combination (BAP + IAA) enhanced the shoot multiplication and shoot growth with both species.
  • Enhance the micropropergation method.

Keywords

  • Dracaena sanderiana,
  • In vitro multiplication,
  • Benzyl Amino Purine,
  • Indole Acetic Acid,
  • Photo-sterilization,
  • Ag/Fe-TiO2 nanoparticles
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