10.57647/jcns.2025.1101.05

Research on the Effects of Vinasse and Humic Acid Along with Optimal Use of Chemical Fertilizers on Soil Nitrogen and Phosphorus Availability and Sugarcane Yield

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  • Seyedeh Zahra Jafari Naeini1,2,
  • Alireza Jafarnejadi*3,
  • Kamran Mohsenifar2,
  • Timoor Babaienejad2,
  • Ali Gholami4,
  1. Department of Soil Science, Khuzestan Science and Research Branch, Islamic Azad University, Ahvaz, Iran.
  2. Department of Soil Science, Ahvaz Branch, Islamic Azad University, Ahvaz, Iran.
  3. Soil and Water Research Department, Khuzestan Agricultural and Natural Resources Research and Education Center, AREEO, Ahvaz, Iran.
  4. Water Studies Research Center, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran.

Received: 2025-01-08

Revised: 2025-02-10

Accepted: 2025-03-12

Published in Issue 2025-03-31

Copyright (c) 2026 Seyedeh Zahra Jafari Naeini, Alireza Jafarnejadi, Kamran Mohsenifar, Timoor Babaienejad, Ali Gholami (Author)

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This work is licensed under a Creative Commons Attribution 4.0 International License.

How to Cite

Jafari Naeini, S. Z., Jafarnejadi, A., Mohsenifar, K., Babaienejad, T., & Gholami, A. (2025). Research on the Effects of Vinasse and Humic Acid Along with Optimal Use of Chemical Fertilizers on Soil Nitrogen and Phosphorus Availability and Sugarcane Yield. Journal of Crop Nutrition Science, 11(1). https://doi.org/10.57647/jcns.2025.1101.05
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Abstract

BACKGROUND: Replacing chemical fertilizers with organic fertilizers can play an important role in crop management. Also, by using organic fertilizers, in addition to reducing the consumption of Nitrogenous chemical fertilizers and triple superphosphate, it is possible to reduce costs and environmental pollution, which is a move towards achieving sustainable agricultural goals.

OBJECTIVES: The aim of this study was to investigate the effect of Vinasse and Humic acid on soil Nitrogen and Phosphorus concentrations under Sugarcane cultivation conditions with chemical inputs.

METHODS: Current research was done via split split plots experiment based on complete randomized block design with three replications. Vinasse at three level (0, 50, and 100 cubic meters per hectare) belonged to main plot, three levels of Humic acid (0, 2.5, and 5 kg.ha-1) belonged to sub plot, and two levels of fertilizer treatment (Recommended advised to consume N, P, K and 50% of Recommended) belonged to sub sub plot.

RESULT: The result of analysis of variance revealed effect of effect of Vinasse, Humic acid, fertilizer and interaction effect of treatments on Nitrogen and Phosphorus concentration at both depth was significant. The highest increase in soil Nitrogen content compared to the control at a depth of (30-60) is 0.084 percent, and the highest average percentage increase in soil Nitrogen content compared to the control at a depth of 0-30 centimeters (0.081 percent) is with the use in treatments (100 m3. ha-1 Vinasse). The highest increase in soil Phosphorus compared to the control at a depth of (0-30 cm) and (30-60 cm) was 7.98 and 6.8 mg Phosphorus per kilogram of soil and with the in treatments (100 m3. ha-1 Vinasse). Consume 5 kg.ha-1 Humic acid achieve highest amount of Nitrogen, Phosphorus content and crop yield in both depths. So we can conclude that Vinasse, along with proper fertilizer management, can compensate for the lack and availability of some Nitrogen and Phosphorus in the soil and lead to a reduction in the consumption of chemical inputs and consequently reduce environmental pollution and by adding the highly nutritious elements Nitrogen, Phosphorus, and organic matter, it contributes significantly to soil fertility and plant growth.

CONCLUSION: Finally according result of current research consume 50 m3.ha-1 Vinasse and 5 kg.ha-1 with 50% recommended amount fertilizer led to produce economic yield and decrease use of conventional fertilizer based on sustainable agriculture and input management.

Keywords

  • Industrial crop, Nutrition, Organic matter, Soil depth, Sugarcane by products
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References

  1. Alavi, N., Daneshpajou, M., Shirmardi, M., Goudarzi, G., Neisi, A. and Babaei, A. A. 2017. Investigating the efficiency of co-composting and vermicomposting of Vinasse with the mixture of Cow manure wastes, bagasse, and natural zeolite. Waste Management, 69, 117-126.
  2. Barahimi, N., Afyuni, M., Karami, M. and Rezaee Nejad, Y. 2009. Cumulative and residual effects of organic amendments on Nitrogen, Phosphorus and Potassium concentrations in soil and Wheat. Journal of Water and Soil Science, 12(46), 803-812.
  3. Behravan, H., Khorasani, R., Fotovat, A., Moezei, A. A. and Taghavi, M. 2020. Effects of Humic acid and Phosphorus fertilizer on root, root hair, Phosphorus uptake and Phosphorus influx in Sugarcane (Saccharum officinarum). Water and Soil, 33(5), 709-721. (Abstract in English) https://doi.org/10.22067/jsw.v33i5.78726
  4. Bouyoucos, G. J. 1962. Hydrometer method improved for making particle size analyses of soils. Agronomy Journal, 54(5), 464-465.
  5. https://doi.org/10.2134/agronj1962.00021962005400050028x
  6. Campiteli, L., Santos, R., Lazarovits, G. and Rigobelo, E. 2018. The impact of applications of Sugarcane filter cake and Vinasse on soil fertility factors in fields having four different crop rotations practices in Brazil. Científica, 46(1), 42-48.
  7. https://doi.org/10.15361/1984-5529.2018v46n1p42-48
  8. Da Silva, V., Oliveira, M. and Ferreira, V. 2018. Stalk yield and nutrients accumulation of sugarcane varieties in three crop cycles. Revista de Ciencias Agrarias, 41.
  9. https://doi.org/10.19084/RCA17051
  10. Desalegn, B., Kebede, E., Legesse, H. and Fite T. 2023. Sugarcane productivity and sugar yield improvement: Selecting variety, Nitrogen fertilizer rate, and bio regulator as a first-line treatment. Heliyon, 9(4), e15520. Doi: 10.1016/j.heliyon.2023.e15520.
  11. Dou, Z., Tang, S., Li, G., Liu, Z., Ding, C., Chen, L., Wang, S. and Ding, Y. 2017. Application of Nitrogen fertilizer at heading stage improves Rice quality under elevated temperature during grain filling stage. Crop Science, 57(4), 2183-2192.
  12. https://doi.org/10.2135/cropsci2016.05.0350.
  13. Garcia, C. F. H., De Souza, R. B., De Souza, C. P., Christofoletti, C. A. and Fontanetti, C. S. 2017. Toxicity of two effluents from agricultural activity: comparing the Geno toxicity of Sugarcane and orange Vinasse. Ecotoxicology and Environmental Safety, 142, 216-221.
  14. Gee, G. W. and Bauder, J. W. 1986. Methods of Soil Analysis: Part I-Physical and Mineralogical Methods. Soil Science Society of America, American Society of Agronomy. https://doi.org/10.2136/sssabookser5.1.2ed.frontmatter
  15. Golchin, A., Rakhsh, F., Salvand, B. and Vatani, A. 2016. Dynamics of Sugar Beet Vinasse in soil and its effect on physicochemical properties of calcareous and acidic soil. Journal of Soil Management and Sustainable Production, 6(2), 151-166. (Abstract in English) Doi: 10.22069/ejsms.2016.3148
  16. Holfold, I. C. R. 1997. Soil Phosphorus: its measurement and its uptake by plants. Australian Journal of Soil Research, 35, 277-239.
  17. Hussain, S., Anwar-ul-Haq, M., Hussain, S., Akram, Z., Afzal, M. and Shabbir, I. 2017. Best suited timing schedule of inorganic NPK fertilizers and its effect on qualitative and quantitative attributes of spring sown Sugarcane (Saccharum officinarum L.), Journal of the Saudi Society of Agricultural Sciences, 16(1), 66-71.
  18. Jafarinia, H., Shabani, A., Safirzadeh, S. and Amiri, M. 2023. Yield and water productivity of Sugarcane under different irrigation intervals, Nitrogen fertilizer levels, and planting methods. Journal of Water and Soil Science. 27(2), 53-69. (Abstract in English) Doi: 10.47176/jwss.27.2.49441
  19. Knudsen, D., Peterson, G. A. and Pratt, P. 1982. Lithium, Sodium and Potassium. In: Page, A. L., Ed., Methods of Soil Analysis, American Society of Agronomy, Madison, pp, 225-246. https://doi.org/https://doi.org/10.2134/agronmonogr9.2.2ed.c13
  20. Kusumaningtyas, R. D., Oktafiani, O., Hartanto, D. and Handayani, P. A. 2018. Effects of solid Vinasse-based organic fertilizer on some growth indices of Tomato plant. Journal Bahan Alam Terbarukan, 6(2), 190-197.
  21. Li, Y., Fang, F., Wei, J., Wu, X., Cui, R., Li, G., Zheng, F. and Tan, D. 2019. Humic acid fertilizer improved soil properties and soil microbial diversity of continuous cropping Peanut: A three-year experiment. Scientific Reports, 9(1), 12014.
  22. https://doi.org/10.1038/s41598-019-48620-4
  23. Mahidi S. S., Hassan G. I., Hussain, A. and Faisul-ur-Rasool, A. 2011. Phosphorus availability issue-its fixation and role of phosphate solubilizing bacteria in phosphate solubilization-case study. Agricultural Science Research Journal, 2, 174-179.
  24. Mahohi, A., Khatin zadeh, H. A., Nouri, M. and Nikfar, D. 2023. Consequences of urea Phosphate fertilizer application on the nutrient status and quantitative and qualitative yield of Sugarcane. Applied Soil Research, 11(2), 13-27. (Abstract in English)
  25. Doi: 10.30466/asr.2023.121362
  26. Makvandi, M., Bakhshandeh, A., Moshatati, A., Moradi Telavat, M. and Khodaei joghan, A. 2024. The effect of combined use of Nitrogen fertilizer and Sugarcane residue compost on Wheat grain quality and yield under terminal heat stress conditions in Ahwaz. Journal of Crops Improvement, 26(1), 51-74. (Abstract in English)
  27. doi: 10.22059/jci.2023.358472.2810
  28. Mehdi, F., Cao, Z., Zhang, S., Gan, Y., Cai, W., Peng, L., Wu, Y., Wang, W. and Yang, B. 2024. Factors affecting the production of sugarcane yield and sucrose accumulation: suggested potential biological solutions. Frontier in Plant Science, 15, 1499021. doi: 10.3389/fpls.2024.1374228
  29. Meteorological Organization. 2019. Metrological data of Khuzestan province (Ahvaz region).
  30. Monjezi, H., R Moradi-Telavat, M., Siadat, S. A., Koochakzadeh, A. and Hamdi, H. 2015. Effect of sugarcane filter muds, chemical and biological fertilizers on absorption of some Macro- and Micro-Elements and heavy, metals by Canola (Brassica napus L.). Journal of Crop Production and Processing, 5(17), 193-202. (Abstract in English)
  31. https://doi.org/10.18869/acadpub.jcpp.5.17.193
  32. Oliveira, W. S., Brito, M. E. B., Alves, R. A. B., Dos Santos Souza, A. and Da Silva, E. G. 2014. Cultivo da cana-de-acucar sob fertirrigacao com vinhaca e adubacao mineral. Revista Verde de Agroecologia e Desenvolvimento Sustentavel, 9, 1-5.
  33. Paksoy, M., Türkmen, Ö. and Dursun, A. 2010. Effects of Potassium and Humic acid on emergence, growth and nutrient contents of Okra (Abelmoschus esculentus L.) seedling under saline soil conditions. African Journal of Biotechnology, 9, 5343-5346.
  34. Pazoki, M., Shaigan, J. and Afshari, A. 2006. Investigation of effluent treatment methods of alcohol production units. Journal of Environmental Studies, 32(39), 12-25.
  35. Prado, R. D. M., Caione, G. and Campos, C. N. S. 2013. Filter Cake and Vinasse as Fertilizers Contributing to Conservation Agriculture. Applied and Environmental Soil Science, 581984. https://doi.org/10.1155/2013/581984
  36. Qetrani, S., Bouray, M. and Oukarroum, A. 2024. Phosphorus mobilization and acquisition in the alkaline-calcareous rhizosphere: A synthesis. Rhizosphere, 30, 100907.
  37. https://doi.org/10.1016/j.rhisph.2024.100907
  38. Raei, Y., Shaghi Sardodood, S. N. and Baghery Pirooz, A. 2013. The effects of chemical and biological fertilizers application on forage Sorghum (Sorghum bicolor L.) yields of different harvests. Journal of Agro-Ecology, 5(3), 231-242.
  39. Raei, Y., Sayyadi Ahmadabad, M., Ghassemi-Golezani, K. and Ghassemi, S. 2020. The effect of biological and chemical Nitrogen fertilizers on Pinto Bean (Phaseolus vulgaris L.) and Black Mustard (Brasassica nigra L.) intercropping. Journal of Agricultural Science and Sustainable Production, 30(3), 21-40. (Abstract in English)
  40. Richards, L. A. 1954. Diagnosis and Improvement of Saline Alkali Soils, Agriculture, 160, Handbook 60. US Department of Agriculture, Washington DC. USA.
  41. Rodrigues Reis, C. E. and Hu, B. 2017. Vinasse from Sugarcane ethanol production: Better treatment or better utilization?. Frontiers in Energy Research, 5(7).
  42. https://doi.org/10.3389/fenrg.2017.00007
  43. Roudgarnejad, S., Samdeliri, M., Mirkalaei, A. M. and Moghaddam, M. N. 2021. The role of Humic acid application on quantitative and qualitative traits of Faba Bean (Vicia faba L.). Gesunde Pflanzen, 73(4), 603-611.
  44. https://doi.org/10.1007/s10343-021-00581-3
  45. Rousta, M. J. and Enayati, K. 2019. The effects of Humic acid application on yield and yield components of Wheat and some chemical properties of a Saline-Sodic soil. Journal of Soil Management and Sustainable Production, 8(4), 95-109.
  46. (Abstract in English) https://doi.org/10.22069/ejsms.2019.14096.1778
  47. Soroush, N., Sayyad, G., Moezi, A. and Khorramian, M. 2011. Movement of different forms of Nitrogen in soil under the influence of different amounts of urea fertilizer in corn irrigation system in Northern Khuzestan. Agricultural Scientific Journal, 34(2), 1-11. (Abstract in English)
  48. Sumner, M. E. and Miller, W. P. 1996. Cation Exchange Capacity and Exchange Coefficients. In: Sparks, D. L., Ed., Methods of Soil Analysis, Part III: Chemical Methods, SSSA Book Series 5, Soil Science Society of America, Madison, Wisconsin, pp, 1201-1230. https://doi.org/https://doi.org/10.2136/sssabookser5.3.c40
  49. Talebizadeh, A. and Marashi, S. K. 2019. Investigating effect different level of fertilizer, biofertilizer and Sugarcane compost on Agro-physiological characteristics of Maize (S.C 703). Journal of Crop Nutrition Science, 5(2): 47-56.
  50. Taleh Farahi, F., Gholamalizadeh, A. and Hemati, A. 2019. The effect of extracted Humic acid from different sources on the characteristics of Sunflower grown in a calcareous soil. Journal of Environmental Stresses in Crop Sciences, 12(2), 617-630. (Abstract in English) Doi: 10.22077/escs.2019.1418.1307
  51. Tejada, M. and Gonzalez, J. L. 2005. Beet Vinasse applied to wheat under dryland conditions affects soil properties and yield. European Journal of Agronomy, 23(4), 336-347.
  52. Walkley, A. and Black, I. A. 1934. An Examination of the Degtjareff Method for Determining Soil Organic Matter, and a Proposed Modification of the Chromic Acid Titration Method. Soil Science, 37(1), 29-38.
  53. https://doi.org/10.1097/00010694-193401000-00003
  54. Yin, J., Deng, C.B., Wang, X. F., Chen, G.L., Mihucz, V. G., Xu, G. P. and Deng, Q. 2018. Effects of Long-Term application of Vinasse on physicochemical properties, heavy metals content and microbial diversity in Sugarcane field soil, Sugar Tech, 21, 62-70.
  55. https://doi.org/10.1007/s12355-018-0630-2
  56. Zarcinas, B. A., Cartwright, B. and B. Spouncer. 1987. Nitric acid digestion and multi-element analysis of plant material by inductively coupled plasma spectrometry. Communication in Soil Science and Plant Analysis, 18, 131-146.
  57. Doi: 10.22067/jag.v5i3.28993