10.57647/ijic.2026.1701.05

Synthesis and Application of a Third-Generation Magnetic Graphene Oxide Nanoadsorbent for High-Efficiency Aflatoxin Removal

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  • Zahra Shafiee1,
  • Fariba Tadayon*1,
  • Rouhollah Karimi-Osboo2,
  • Homayon Ahmad Panahi3,
  • Elham Moniri4,
  1. Department of Chemistry, NT.C., Islamic Azad University, Tehran, Iran
  2. Mycotoxins Research Laboratory, Agricultural Research Education and Extension Organization (AREEO), Iranian Research Institute of Plant Protection, Shiraz, Iran
  3. Department of Chemistry, CT.C., Islamic Azad University, Tehran, Iran
  4. Department of Chemistry, VaP.C., Islamic Azad University, Varamin, Iran

Received: 2026-01-19

Revised: 2026-02-03

Accepted: 2026-03-18

Published in Issue 2026-03-31

Copyright (c) 2026 Zahra Shafiee, Fariba Tadayon, Rouhollah Karimi-Osboo, Homayon Ahmad Panahi, Elham Moniri (Author)

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

How to Cite

Shafiee, Z., Tadayon, F., Karimi-Osboo, R., Panahi, H. A., & Moniri, E. (2026). Synthesis and Application of a Third-Generation Magnetic Graphene Oxide Nanoadsorbent for High-Efficiency Aflatoxin Removal. International Journal of Industrial Chemistry, 17(1). https://doi.org/10.57647/ijic.2026.1701.05
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Abstract

A novel graphene oxide (GO)-based nanocomposite was developed for efficient adsorption and extraction of aflatoxins from contaminated solutions. Synthesis began with the activation of cyanuric chloride, followed by extension with the ligand para-aminobenzoic acid over three generations.To enhance adsorption efficiency and facilitate separation, the final adsorbent was coated with magnetic nanoparticles (Fe₃O₄), yielding a magnetic nanocomposite. The structure and physicochemical properties of the nanocomposite were characterized using FTIR, TGA, XRD, FE-SEM, EDX, and VSM. The adsorbent's performance in aflatoxin extraction was evaluated by HPLC, which showed high adsorption capacity and recovery efficiency. The adsorption isotherm data fit the Langmuir model well (R² = 0.9977), indicating uniform monolayer adsorption. A pseudo-second-order model (R² = 0.9958) further confirmed that the experimental data were consistent with the adsorption kinetics.The synthesized nanoadsorbent demonstrated exceptional efficiency in removing aflatoxins from contaminated samples, underscoring its potential as an effective material for sample pretreatment and separation in food and environmental contexts.

Keywords

  • Aflatoxin adsorption,
  • Dendrimer,
  • Graphene oxide,
  • Magnetic nanocomposite,
  • Nanoadsorbent
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References

  1. R. Karami-Osboo, M. Mirabolfathy, R. Kamran, M. Shetab-Boushehri, S. Sarkari, Aflatoxin B1 in maize harvested over 3 years in Iran, Food Control, 23 (2012) 271-274.
  2. R. Karami-Osboo, M. Mirabolfathi, A novel dispersive nanomagnetic particle solid-phase extraction method to determine aflatoxins in nut and cereal samples, Food Analytical Methods, 10 (2017) 4086-4093.
  3. S. Marin, A. Ramos, G. Cano-Sancho, V. Sanchis, Mycotoxins: Occurrence, toxicology, and exposure assessment, Food and chemical toxicology, 60 (2013) 218-237.
  4. M. Mirabolfathy, R.K. Osboo, V. Rahjoo, Important mycotoxins, Iran status, Annu. Rev. Res, 5 (2019) 1-10.
  5. Y.Y. Gong, S. Watson, M.N. Routledge, Aflatoxin exposure and associated human health effects, a review of epidemiological studies, Food safety, 4 (2016) 14-27.
  6. G. Miklós, C. Angeli, Á. Ambrus, A. Nagy, V. Kardos, A. Zentai, K. Kerekes, Z. Farkas, Á. Jóźwiak, T. Bartók, Detection of aflatoxins in different matrices and food-chain positions, Frontiers in Microbiology, 11 (2020) 1916.
  7. F. Wu, Global impacts of aflatoxin in maize: trade and human health, World Mycotoxin Journal, 8 (2015) 137-142.
  8. Tavassoty Kheiry B.,Tadayon F., Ahmad Panahi H., Moniri E., Design of Temperature-Sensitive Hydrogel Nanoparticles and Investigation of Their Application in the Development of a New Generation of Anti-Cancer Drugs with Optimization Conditions by Central Composite Design. Int. J. Ind. Chem.,2025; 16(2). 1-14. http://doi.org/10.57647/j.ijic.2025.1602.12
  9. J.H. Williams, T.D. Phillips, P.E. Jolly, J.K. Stiles, C.M. Jolly, D. Aggarwal, Human aflatoxicosis in developing countries: a review of toxicology, exposure, potential health consequences, and interventions, The American journal of clinical nutrition, 80 (2004) 1106-1122.
  10. H. Contraception, H. Therapy, IARC monographs on the evaluation of carcinogenic risks to humans, Lyon: International Agency for Research on Cancer, (1999).
  11. Torabi Fard N, Tadayon F, Ahmad Panahi H, Moniri E. Synthesis,characterization and application of a novel three-dimensional magnetic graphene oxide decorated with polyester dendrimers for detection of donepezil hydrochloride in pharmaceutical formulation and biological fluid. Journal of Molecular Liquids. 2022; 365:118149. http://doi.org/10.1016/j.molliq.2021.118149
  12. M. Selin, S. Nummelin, J. Deleu, J. Ropponen, T. Viitala, M. Lahtinen, J. Koivisto, J. Hirvonen, L. Peltonen, M.A. Kostiainen, High-generation amphiphilic Janus-dendrimers as stabilizing agents for drug suspensions, Biomacromolecules, 19 (2018) 3983-3993.
  13. M. Liu, K. Kono, J.M. Fréchet, Water-soluble dendritic unimolecular micelles:: Their potential as drug delivery agents, Journal of Controlled Release, 65 (2000) 121-131.
  14. M. Inagaki, F. Kang, M. Toyoda, H. Konno, Graphene: Synthesis and preparation, Advanced materials science and engineering of carbon, (2014) 41-65.
  15. J. Liu, L. Cui, D. Losic, Graphene and graphene oxide as new nanocarriers for drug delivery applications, Acta biomaterialia, 9 (2013) 9243-9257.
  16. M.Y. Alazaiza, A. Albahnasawi, G.A. Ali, M.J. Bashir, N.K. Copty, S.S.A. Amr, M.F. Abushammala, T. Al Maskari, Recent advances of nanoremediation technologies for soil and groundwater remediation: A review, Water, 13 (2021) 2186.
  17. L. Giraldo, A. Erto, J.C. Moreno-Piraján, Magnetite nanoparticles for removal of heavy metals from aqueous solutions: synthesis and characterization, Adsorption, 19 (2013) 465-474.
  18. F. Yang, J. Li, T. Chen, W. Ren, C. Gao, J. Lin, C. Xu, X. Ma, J. Xing, H. Bao, Applications of magnetic nanoparticles for boundarics in biomedicine, Fundamental Research, (2025).
  19. R. Karami-Osboo, L. Faramarz, Rapid Aflatoxin Detection in Black Tea Using Fe3O4 Magnetic Nanoparticle, Food Analytical Methods, 17 (2024) 1189-1194.
  20. X. Liu, Z. Ma, J. Xing, H. Liu, Preparation and characterization of amino–silane modified superparamagnetic silica nanospheres, Journal of Magnetism and magnetic Materials, 270 (2004) 1-6.
  21. Y. Liu, S. Huang, X. Zhao, Y. Zhang, Fabrication of three-dimensional porous β-cyclodextrin/chitosan functionalized graphene oxide hydrogel for methylene blue removal from aqueous solution, Colloids and Surfaces A: Physicochemical and Engineering Aspects, 539 (2018) 1-10.
  22. D.R. Dreyer, S. Park, C.W. Bielawski, R.S. Ruoff, The chemistry of graphene oxide, Chemical society reviews, 39 (2010) 228-240.
  23. S. Stankovich, D.A. Dikin, G.H. Dommett, K.M. Kohlhaas, E.J. Zimney, E.A. Stach, R.D. Piner, S.T. Nguyen, R.S. Ruoff, Graphene-based composite materials, nature, 442 (2006) 282-286.
  24. A. Sardinha, FTIR Spectra of Graphene Oxide, Instituto Nacional de Pesquisas Espaciais: São José dos Campos, São Paulo, Brazil, (2019).
  25. C.C. Berry, A.S. Curtis, Functionalisation of magnetic nanoparticles for applications in biomedicine, Journal of physics D: Applied physics, 36 (2003) R198.
  26. D. Gao, D.-D. Wang, Q.-F. Fu, L.-J. Wang, K.-L. Zhang, F.-Q. Yang, Z.-N. Xia, Preparation and evaluation of magnetic molecularly imprinted polymers for the specific enrichment of phloridzin, Talanta, 178 (2018) 299-307.
  27. O. Semong, Development of an aflatoxin B1 specific molecularly imprinted solid phase extraction sorbent for the selective pre-concentration of toxic aflatoxin B1 from child weaning food, Tsabana, in, Botswana International University of Science and Technology (Botswana), 2016.
  28. Z.S.G. Hashemipour, F. Tadayon, O.R. KARAMI, Preparation of Magnetic Nano Composite Modified with Orange Peel for Adsorption of Vitamin B5 from Aqueous Solution, (2022).
  29. J.I. Goldstein, D.E. Newbury, J.R. Michael, N.W. Ritchie, J.H.J. Scott, D.C. Joy, Scanning electron microscopy and X-ray microanalysis, springer, 2017.
  30. T. Hatakeyama, F.i. Quinn, Thermal analysis: fundamentals and applications to polymer science, [sl], 1999.
  31. N. Nakagiri, M. Manghnani, L. Ming, S. Kimura, Crystal structure of magnetite under pressure, Physics and Chemistry of Minerals, 13 (1986) 238-244.
  32. T.W. Weber, R.K. Chakravorti, Pore and solid diffusion models for fixed-bed adsorbers, AIChE Journal, 20 (1974) 228-238.
  33. H. Freundlich, Über die adsorption in lösungen, Zeitschrift für physikalische Chemie, 57 (1907) 385-470.
  34. A. Boisen, S. Dahl, J.K. Nørskov, C.H. Christensen, Why the optimal ammonia synthesis catalyst is not the optimal ammonia decomposition catalyst, Journal of Catalysis, 230 (2005) 309-312.
  35. J. Rouquerol, F. Rouquerol, P. Llewellyn, G. Maurin, K. Sing, Adsorption by powders and porous solids: principles, methodology and applications, Academic press, 2013.
  36. F.-C. Wu, B.-L. Liu, K.-T. Wu, R.-L. Tseng, A new linear form analysis of Redlich–Peterson isotherm equation for the adsorptions of dyes, Chemical Engineering Journal, 162 (2010) 21-27.
  37. K.Y. Foo, B.H. Hameed, Insights into the modeling of adsorption isotherm systems, Chemical engineering journal, 156 (2010) 2-10.
  38. A.B. Albadarin, M. Charara, B.J.A. Tarboush, M. Ahmad, T.A. Kurniawan, M. Naushad, G.M. Walker, C. Mangwandi, Mechanism analysis of tartrazine biosorption onto masau stones; a low cost by-product from semi-arid regions, Journal of Molecular Liquids, 242 (2017) 478-483.
  39. S. Suganya, P.S. Kumar, Kinetic and thermodynamic analysis for the redemption of effluents containing Solochrome Black T onto powdered activated carbon: a validation of new solid-liquid phase equilibrium model, Journal of Molecular Liquids, 259 (2018) 88-101.
  40. Z. Berizi, S.Y. Hashemi, M. Hadi, A. Azari, A.H. Mahvi, The study of non-linear kinetics and adsorption isotherm models for Acid Red 18 from aqueous solutions by magnetite nanoparticles and magnetite nanoparticles modified by sodium alginate, Water Science and Technology, 74 (2016) 1235-1242.