10.1007/s40095-014-0157-6

Optimization of ethanol fermentation from discarded carrots using immobilized Saccharomyces cerevisiae

HTML PDF
  • Adriana L. Clementz1,
  • Nora R. Aimaretti2,
  • Debora Manuale1,
  • Agustín Codevilla3,
  • Juan C. Yori*1,
  1. Facultad de Ingeniería Química, Instituto de Investigaciones en Catálisis y Petroquímica, Universidad Nacional del Litoral, CONICET, Santa Fe, Provincia de Santa Fe, 3000, AR
  2. Facultad de Ingeniería Química, Instituto de Investigaciones en Catálisis y Petroquímica, Universidad Nacional del Litoral, CONICET, Santa Fe, Provincia de Santa Fe, 3000, AR Laboratorio de Investigaciones Aplicadas, Facultad de Química, Universidad del Centro Educativo Latinoamericano, Rosario, 2000, AR
  3. Laboratorio de Investigaciones Aplicadas, Facultad de Química, Universidad del Centro Educativo Latinoamericano, Rosario, 2000, AR
Cover Image

Published in Issue 2014-12-18

How to Cite

Clementz, A. L., Aimaretti, N. R., Manuale, D., Codevilla, A., & Yori, J. C. (2014). Optimization of ethanol fermentation from discarded carrots using immobilized Saccharomyces cerevisiae. International Journal of Energy and Environmental Engineering, 6(2 (June 2015). https://doi.org/10.1007/s40095-014-0157-6
Crossref
Scopus
Google Scholar
Europe PMC

HTML views: 34

PDF views: 109

Abstract

Abstract Discarded carrots are a valuable source of biomass amenable for valorization. Their use as raw material for ethanol production by fermentation, using yeasts immobilized in Calcium alginate, was proposed. The biocatalyst immobilization method, the existence of internal and external mass transfer limitations, the effect of the initial pH and the reuse of immobilized yeasts were particularly evaluated. Results indicate that beads made with a 2 % solution of Sodium alginate and a 30 % solution of Saccharomyces cerevisiae were strong enough to allow an efficient nutrient transfer into the matrix and to prevent cell leaking. A stirring rate of 200 rpm was needed to avoid external mass transfer limitations. These beads were used in three successive fermentations. An initial pH of 5.5 reached the best fermentation parameters. Non-enriched, non-sterile carrot must was fermented through immobilized yeasts; and values of ethanol concentration (29.9 g L −1 ), Y p/s (0.409 g g −1 ), and productivity (7.45 g L −1  h −1 ) were obtained. These values were similar to those registered when free cells were used.

Keywords

  • Bioethanol,
  • Carrot discard,
  • Immobilization,
  • Calcium alginate bead,
  • Reuse
HTML PDF

References

  1. Ghorbani et al. (2011) Cane molasses fermentation for continuous ethanol production in an immobilized cells reactor by Saccharomyces cerevisiae (pp. 503-509) https://doi.org/10.1016/j.renene.2010.07.016
  2. Cardona and Sánchez (2007) Fuel ethanol production: process design trends and integration opportunities (pp. 2415-2457) https://doi.org/10.1016/j.biortech.2007.01.002
  3. Talebnia et al. (2010) Production of bioethanol from wheat straw: an overview on pretreatment, hydrolysis and fermentation (pp. 4744-4753) https://doi.org/10.1016/j.biortech.2009.11.080
  4. Brethauer and Wyman (2010) Review: continuous hydrolysis and fermentation for cellulosic ethanol production (pp. 4862-4874) https://doi.org/10.1016/j.biortech.2009.11.009
  5. González-García et al. (2009) Environmental aspects of ethanol-based fuels from Brassica carinata: a case study of second generation ethanol (pp. 2613-2620) https://doi.org/10.1016/j.rser.2009.06.004
  6. Aimaretti et al. (2012) Production of bioethanol from carrot discards (pp. 727-732) https://doi.org/10.1016/j.biortech.2012.08.035
  7. Aimaretti and Ybalo (2012) Valorization of carrot and yeast discards for the obtention of ethanol (pp. 18-23) https://doi.org/10.1016/j.biombioe.2012.03.022
  8. Aimaretti et al. (2013) Sustainable fermentation processing of two revalorized agro-industrial discards: carrot and brewer’s yeast (pp. 1-7)
  9. Vasconcelos et al. (2004) Continuous ethanol production using yeast immobilized on sugar-cane stalks (pp. 357-365) https://doi.org/10.1590/S0104-66322004000300002
  10. Draget et al. (1997) Alginate based new materials (pp. 47-55) https://doi.org/10.1016/S0141-8130(97)00040-8
  11. Idris and Suzana (2006) Effect of sodium alginate concentration, bead diameter, initial pH and temperature on lactic acid production from pineapple waste using immobilized Lactobacillus delbrueckii (pp. 1117-1123) https://doi.org/10.1016/j.procbio.2005.12.002
  12. Yan et al. (2001) Lactic acid fermentation from enzyme-thinned starch with immobilized Lactobacillus amylovorus 15(2) (pp. 59-63)
  13. Carvalho et al. (2003) Kylitol production by Ca alginate entrapped cells: comparison of different fermentation systems (pp. 553-559) https://doi.org/10.1016/S0141-0229(03)00007-3
  14. Prasad and Mishra (1995) On the kinetics and effectiveness of Immobilized whole cell batch cultures (pp. 269-275) https://doi.org/10.1016/0960-8524(95)00096-4
  15. Najafpour et al. (2004) Ethanol fermentation in an immobilized cell reactor using Saccharomyces cerevisiae 92(3) (pp. 251-260) https://doi.org/10.1016/j.biortech.2003.09.009
  16. Yu et al. (2004) Optimization of media conditions for the production of ethanol from sweet sorghum juice by immobilized Saccharomyces cerevisiae (pp. 521-526) https://doi.org/10.1016/j.biombioe.2008.08.020
  17. Colin and Bjorn (2002) Cambridge University Press
  18. Park and Chang (2000) Microencapsulation of microbial cells (pp. 303-319) https://doi.org/10.1016/S0734-9750(00)00040-9
  19. Lee et al. (2011) Enhanced production of bioethanol and ultrastructural characteristics of reused Saccharomyces cerevisiae immobilized calcium alginate beads (pp. 8191-8198) https://doi.org/10.1016/j.biortech.2011.06.063
  20. Gilson and Thomas (1995) Ethanol production by alginate immobilized yeast in a fluidized bed bioreactor (pp. 38-45) https://doi.org/10.1002/jctb.280620106
  21. Ercan et al. (2013) Optimization of ethanol production from carob pod extract using immobilized Saccharomyces cerevisiae cells in a stirred tank bioreactor (pp. 365-371) https://doi.org/10.1016/j.biortech.2012.09.006
  22. Roukas (1994) Continuous ethanol production from carob pod extract by immobilized Saccharomyces cerevisiae in a packed-bed reactor (pp. 387-393) https://doi.org/10.1002/jctb.280590412
  23. Kourkoutas et al. (2004) Immobilization technologies and support materials suitable inalcohol beverages production: a review (pp. 377-397) https://doi.org/10.1016/j.fm.2003.10.005
  24. Buzas et al. (1989) Influence of pH on the growth and ethanol production of free and immobilized Saccharomyces cerevisiae cells (pp. 882-884) https://doi.org/10.1002/bit.260340620