10.1186/2251-6832-4-14

Lipid production of Chlorella vulgaris and Pseudokirchneriella subcapitata

  1. LEPAE, Departamento de Engenharia Química, Faculdade de Engenharia, Universidade do Porto, Porto, 4200-465, PT
Cover Image

Published in Issue 2013-04-03

How to Cite

Gonçalves, A. L., Pires, J. C., & Simões, M. (2013). Lipid production of Chlorella vulgaris and Pseudokirchneriella subcapitata. International Journal of Energy and Environmental Engineering, 4(1 (December 2013). https://doi.org/10.1186/2251-6832-4-14

HTML views: 31

PDF views: 103

Abstract

Abstract Background The depletion of fossil fuel reserves has stimulated the search for sustainable sources of energy that are carbon-neutral or renewable. In this context, microalgae are a promising energetic resource. They are photosynthetic microorganisms that use CO 2 as carbon source, with high specific growth rates. Furthermore, some species present high lipid content that can be easily converted into biodiesel. Accordingly, this study aims to analyze the effect of light supply (one of the most important culture parameters) on lipid production of selected microalgae, Chlorella vulgaris and Pseudokirchneriella subcapitata. Methods Both microalgal species were cultured under different light irradiance values (36, 72, 96, and 126 μE m −2 s −1 ) and for each light irradiance value, three light/dark ratios (10:14, 14:10, and 24:0) were tested. Lipid contents of both microalgae were then determined using a recently developed colorimetric method. Results/Conclusions P. subcapitata presented higher lipid productivity than C. vulgaris . High lipid concentration was achieved in microalgal cultures with higher light irradiance values. However, for 96 and 126 μE m −2 s −1 , lipid productions of both microalgae were not significantly higher than with 72 μE m −2 s −1 , which means that microalgal light saturation point may be achieved.

Keywords

  • Chlorella vulgaris,
  • Pseudokirchneriella subcapitata,
  • Lipid production,
  • Light effect,
  • Microalgae

References

  1. Oyedepo (2012) Efficient energy utilization as a tool for sustainable development in Nigeria (pp. 11-22) https://doi.org/10.1186/2251-6832-3-11
  2. Betiku and Adepoju (2013) Methanolysis optimization of sesame (Sesamum indicum) oil to biodiesel and fuel quality characterization (pp. 9-16) https://doi.org/10.1186/2251-6832-4-9
  3. Demirbas and Demirbas (2011) Importance of algae oil as a source of biodiesel (pp. 163-170) https://doi.org/10.1016/j.enconman.2010.06.055
  4. Raj and Kandasamy (2012) Tamanu oil - an alternative fuel for variable compression ratio engine (pp. 18-25) https://doi.org/10.1186/2251-6832-3-18
  5. Gonçalves et al. (2013) Biodiesel from microalgal oil extraction Springer
  6. Kim et al. (2013) Evaluation of food waste disposal options in terms of global warming and energy recovery: Korea (pp. 1-12) https://doi.org/10.1186/2251-6832-4-1
  7. Eddine and Salah (2012) Solid waste as renewable source of energy: current and future possibility in Algeria (pp. 17-28) https://doi.org/10.1186/2251-6832-3-17
  8. Chisti (2007) Biodiesel from microalgae (pp. 294-306) https://doi.org/10.1016/j.biotechadv.2007.02.001
  9. Mercer and Armenta (2011) Developments in oil extraction from microalgae (pp. 539-547) https://doi.org/10.1002/ejlt.201000455
  10. Brennan and Owende (2010) Biofuels from microalgae—a review of technologies for production, processing, and extractions of biofuels and co-products (pp. 557-577) https://doi.org/10.1016/j.rser.2009.10.009
  11. Liu et al. (2008) Effect of iron on growth and lipid accumulation in Chlorella vulgaris (pp. 4717-4722) https://doi.org/10.1016/j.biortech.2007.09.073
  12. Weldy and Huesemann (2007) Lipid production by Dunaliella salina in batch culture: effects of nitrogen limitation and light intensity (pp. 115-122)
  13. Bligh and Dyer (1959) A rapid method of lipid extraction and purification (pp. 911-917) https://doi.org/10.1139/o59-099
  14. Goutx et al. (1990) An application of Iatroscan thin-layer chromatography with flame ionization detection—lipid classes of microorganisms as biomarkers in the marine environment (pp. 1231-1237) https://doi.org/10.1016/0146-6380(90)90158-V
  15. Volkman et al. (1989) Fatty acid and lipid composition of 10 species of microalgae used in mariculture (pp. 219-240) https://doi.org/10.1016/0022-0981(89)90029-4
  16. Molina Grima et al. (2003) Recovery of microalgal biomass and metabolites: process options and economics (pp. 491-515) https://doi.org/10.1016/S0734-9750(02)00050-2
  17. Capasso et al. (2003) A colorimetric assay for determination of cell viability in algal cultures (pp. 133-138) https://doi.org/10.1016/S1389-0344(03)00037-6
  18. Chen and Vaidyanathan (2012) A simple, reproducible and sensitive spectrophotometric method to estimate microalgal lipids (pp. 67-72) https://doi.org/10.1016/j.aca.2012.02.049
  19. Wawrik and Harriman (2010) Rapid, colorimetric quantification of lipid from algal cultures (pp. 262-266) https://doi.org/10.1016/j.mimet.2010.01.016
  20. Lee et al. (1998) Rapid method for the determination of lipid from the green alga Botryococcus braunii (pp. 553-556) https://doi.org/10.1023/A:1008811716448
  21. Chen et al. (2009) A high throughput Nile red method for quantitative measurement of neutral lipids in microalgae (pp. 41-47) https://doi.org/10.1016/j.mimet.2009.01.001
  22. Genicot et al. (2005) The use of a fluorescent dye, Nile red, to evaluate the lipid content of single mammalian oocytes (pp. 1181-1194) https://doi.org/10.1016/j.theriogenology.2004.06.006
  23. Izard and Limberger (2003) Rapid screening method for quantitation of bacterial cell lipids from whole cells (pp. 411-418) https://doi.org/10.1016/S0167-7012(03)00193-3
  24. Elsey et al. (2007) Fluorescent measurement of microalgal neutral lipids (pp. 639-642) https://doi.org/10.1016/j.mimet.2006.11.008
  25. Bertozzini et al. (2011) Application of the standard addition method for the absolute quantification of neutral lipids in microalgae using Nile red (pp. 17-23) https://doi.org/10.1016/j.mimet.2011.06.018
  26. Duncombe (1963) The colorimetric micro-determination of long-chain fatty acids (pp. 7-10) https://doi.org/10.1042/bj0880007
  27. Ayers (1956) Estimation of the higher fatty acids C7-C18 (pp. 77-83) https://doi.org/10.1016/0003-2670(56)80014-7
  28. Iwayama (1959) New colorimetric determination of higher fatty acids (pp. 552-554)
  29. Unknown (2011) OECD Publishing
  30. Kwon et al. (2005) Effect of algae on fouling and efficiency of UF membranes (pp. 203-214) https://doi.org/10.1016/j.desal.2004.11.068
  31. Jacob-Lopes et al. (2009) Effect of light cycles (night/day) on CO2 fixation and biomass production by microalgae in photobioreactors (pp. 306-310) https://doi.org/10.1016/j.cep.2008.04.007
  32. Bhola et al. (2011) Effects of parameters affecting biomass yield and thermal behaviour of Chlorella vulgaris (pp. 377-382) https://doi.org/10.1016/j.jbiosc.2010.11.006
  33. Molina Grima et al. (2001) Tubular photobioreactor design for algal cultures (pp. 113-131) https://doi.org/10.1016/S0168-1656(01)00353-4
  34. Chisti (2008) Biodiesel from microalgae beats bioethanol (pp. 126-131) https://doi.org/10.1016/j.tibtech.2007.12.002
  35. Lee et al. (2010) Comparison of several methods for effective lipid extraction from microalgae (pp. S75-S77) https://doi.org/10.1016/j.biortech.2009.03.058
  36. Ranjan et al. (2010) Mechanistic assessment of microalgal lipid extraction (pp. 2979-2985) https://doi.org/10.1021/ie9016557
  37. Solovchenko et al. (2008) Effects of light intensity and nitrogen starvation on growth, total fatty acids and arachidonic acid in the green microalga Parietochloris incisa (pp. 245-251) https://doi.org/10.1007/s10811-007-9233-0
  38. Renaud et al. (1991) Effect of light intensity on the proximate biochemical and fatty acid composition of Isochrysis sp. and Nannochloropsis oculata for use in tropical aquaculture (pp. 43-53) https://doi.org/10.1007/BF00003918
  39. Asada et al. (1994) Production and action of active oxygen species in photosynthetic tissues (pp. 77-104) CRC
  40. Carvalho et al. (2011) Light requirements in microalgal photobioreactors: an overview of biophotonic aspects (pp. 1275-1288) https://doi.org/10.1007/s00253-010-3047-8
  41. Falkouski and Raven (2007) Princeton University Press